The Function of the Broad-Complex During Drosophila melanogaster Oogenesis

Tzolovsky, George; Deng, Wu-Min; Schlitt, Thomas; Bownes, Mary

doi:10.1093/genetics/153.3.1371

Cited by 96 publications

(6 citation statements)

References 33 publications

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“…The polar cell cluster was identified by a known marker upd1 (31) at default resolution value (R=1). Three subpopulations of stretch cells were distinguished by several marker genes, including peb (32), sosie (33), br (34), past1, Glut4EF and Vha16-1 (35) at 0.5 resolution value (R=0.5), and the terminal follicle cell cluster was identified by the expression of past1 . The expression of SPARC and Jupiter was used to identify the 1-4 stage of main body follicle cell (1-4 MBFC) (30).…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Sun

Nystul

Zhong

2022

Preprint

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Drosophila ovarian germline stem cells (GSCs) are powerful model for stem cell research. However, due to the scarcity of GSCs in ovarian tissue, it is difficult to obtain the transcriptional profile of GSCs and identify novel GSC markers. In this study, we took advantage of single cell RNA sequencing (scRNA-seq) to profile the germline cells and somatic cells in wild type Drosophila ovary. We then performed an in vivo RNAi screen and network analysis to identify genes that are involved in the early stages of germ cell differentiation. We identified 33 genes with limited expression during early germ cell development and identified 19 genes that potentially regulate germ cell differentiation. Among these, an uncharacterized gene, which we named eggplant (eggpl), is highly expressed in GSC and downregulated in early daughter cells. Upon RNAi knockdown of eggpl, we observed an increase in germ cell proliferation, an accumulation of cysts in the early mitotic (2- and 4-cell) stages and an increase in overall ovary size compared to control when flies were maintained on a standard diet. In flies fed a rich yeast diet, the expression of eggpl was significantly lower and the effects of eggpl RNAi were suppressed, suggesting that downregulation of eggpl may link nutritional status to germ cell proliferation and differentiation. We also found that the matrix metalloproteases, Mmp1 and Mmp2 as well as the tissue inhibitor of metalloproteases (Timp) are additional regulators of eggpl. Collectively, this study provides new insight into the signaling networks that regulate early germ cell development and identifies eggpl as a key player in this process.

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Section: Resultsmentioning

confidence: 99%

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Sun

Nystul

Zhong

2022

Preprint

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“…Making the simplest assumption that a single factor is affected by Lov to produce both phenotypes, we sought to identify regulators that affect both eggshell synthesis and eggshell morphogenesis and for which action in the follicle cells is controlled by signals from the germline. Two transcriptional regulators, Broad [33,56] and Tramtrack [57][58][59], meet these criteria. Interestingly these are both BTB transcriptional regulators, offering the possibility, as discussed for Fruitless above, that Lov misregulates function by direct protein interaction.…”

Section: The Lov 66 Neomorphic Phenotypementioning

confidence: 99%

“…Further, mutation of broad and dominant interference with ecdysone signaling produce eggshell phenotypes similar to those seen with lov 66 . In particular i) the fragile chorions and short/ malformed/branched DAs from mothers mutant for the rbp class of broad mutations [56] are similar to the eggs from lov 66 mothers and ii) the short eggs with stubby DAs and cupshaped dorsal anteriors produced by dominant negative inhibition of ecdysone receptor function in the follicle cells, or loss of ecdysone production, are like the Class 3 eggs identified for lov 66 [61]. These findings suggest that the neomorphic action of Lov in the ovarian germline affects ecdysone signaling and that downstream effects on follicle cell function include disruption of the action of Broad.…”

Section: The Lov 66 Neomorphic Phenotypementioning

confidence: 99%

Correction: The Drosophila BTB Domain Protein Jim Lovell Has Roles in Multiple Larval and Adult Behaviors

Bjorum¹,

Simonette²,

Alanis³

et al. 2013

PLoS ONE

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Innate behaviors have their origins in the specification of neural fates during development. Within Drosophila, BTB (Bric-abrac,Tramtrack, Broad) domain proteins such as Fruitless are known to play key roles in the neural differentiation underlying such responses. We previously identified a gene, which we have termed jim lovell (lov), encoding a BTB protein with a role in gravity responses. To understand more fully the behavioral roles of this gene we have investigated its function through several approaches. Transcript and protein expression patterns have been examined and behavioral phenotypes of new lov mutations have been characterized. Lov is a nuclear protein, suggesting a role as a transcriptional regulator, as for other BTB proteins. In late embryogenesis, Lov is expressed in many CNS and PNS neurons. An examination of the PNS expression indicates that lov functions in the late specification of several classes of sensory neurons. In particular, only two of the five abdominal lateral chordotonal neurons express Lov, predicting functional variation within this highly similar group. Surprisingly, Lov is also expressed very early in embryogenesis in ways that suggests roles in morphogenetic movements, amnioserosa function and head neurogenesis. The phenotypes of two new lov mutations that delete adjacent non-coding DNA regions are strikingly different suggesting removal of different regulatory elements. In lov 47 , Lov expression is lost in many embryonic neurons including the two lateral chordotonal neurons. lov 47 mutant larvae show feeding and locomotor defects including spontaneous backward movement. Adult lov 47 males perform aberrant courtship behavior distinguished by courtship displays that are not directed at the female. lov 47 adults also show more defective negative gravitaxis than the previously isolated lov 91Y mutant. In contrast, lov 66 produces largely normal behavior but severe female sterility associated with ectopic lov expression in the ovary. We propose a negative regulatory role for the DNA deleted in lov 66 .

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“…Moreover, the BR-C gene is essential for choriogenesis and endoreplication in D . melanogaster [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

“…Ecdysone response genes are required for vitellogenesis, with ecdysone receptor binding sites and transcriptional regulators E74 and E75 identified in the 5 0 upstream regulatory region of the vitellogenin (Vg) gene [30,31]. Moreover, the BR-C gene is essential for choriogenesis and endoreplication in D. melanogaster [32].…”

Section: Introductionmentioning

confidence: 99%

A critical role for ecdysone response genes in regulating egg production in adult female Rhodnius prolixus

2023

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Ecdysteroids control ovary growth and egg production through a complex gene hierarchy. In the female Rhodnius prolixus, a blood-gorging triatomine and the vector of Chagas disease, we have identified the ecdysone response genes in the ovary using transcriptomic data. We then quantified the expression of the ecdysone response gene transcripts (E75, E74, BR-C, HR3, HR4, and FTZ-F1) in several tissues, including the ovary, following a blood meal. These results confirm the presence of these transcripts in several tissues in R. prolixus and show that the ecdysone response genes in the ovary are mostly upregulated during the first three days post blood meal (PBM). Knockdown of E75, E74, or FTZ-F1 transcripts using RNA interference (RNAi) was used to understand the role of the ecdysone response genes in vitellogenesis and egg production. Knockdown significantly decreases the expression of the transcripts for the ecdysone receptor and Halloween genes in the fat body and the ovaries and reduces the titer of ecdysteroid in the hemolymph. Knockdown of each of these transcription factors typically alters the expression of the other transcription factors. Knockdown also significantly decreases the expression of vitellogenin transcripts, Vg1 and Vg2, in the fat body and ovaries and reduces the number of eggs produced and laid. Some of the laid eggs have an irregular shape and smaller volume, and their hatching rate is decreased. Knockdown also influences the expression of the chorion gene transcripts Rp30 and Rp45. The overall effect of knockdown is a decrease in number of eggs produced and a severe reduction in number of eggs laid and their hatching rate. Clearly, ecdysteroids and ecdysone response genes play a significant role in reproduction in R. prolixus.

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The Function of the Broad-Complex During Drosophila melanogaster Oogenesis

Cited by 96 publications

References 33 publications

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Single-cell RNA sequencing identifies regulators of differentiation and nutritional cues in Drosophila female germ cells

Correction: The Drosophila BTB Domain Protein Jim Lovell Has Roles in Multiple Larval and Adult Behaviors

A critical role for ecdysone response genes in regulating egg production in adult female Rhodnius prolixus

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