The function of small RNA in <i>Pseudomonas aeruginosa</i>

Liu, Pei; Changwu, Yue; Liu, Lihua; Gao, Can; Lyu, Yuhong; Deng, Shanshan; Tian, Hongying; Jia, Xu

doi:10.7717/peerj.13738

Cited by 1 publication

(1 citation statement)

References 122 publications

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“…Several other sRNAs have been described as pleiotropic modulators of bacterial virulence, such as P. aeruginosa RsmZ, ReaL, ErsA, and SrbA (Liu et al 2022); Staphylococcus aureus RsaA and Teg49 (Menard et al 2022); and E. coli and Salmonella enterica McaS, OmrA, and OxyS (Mitra and Mukhopadhyay 2023). Therefore, whilst most of these sRNAs positively modulate bacterial virulence and biofilm formation, RIT11b was downregulated when B. cenocepacia was infecting C. elegans and its overexpression reduced bacterial virulence and biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Identification of Burkholderia cenocepacia non-coding RNAs expressed during Caenorhabditis elegans infection

Pita,

Feliciano,

Leitão

2023

Appl Microbiol Biotechnol

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Small non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Despite the identification of hundreds of bacterial sRNAs, their roles on bacterial physiology and virulence remain largely unknown, as is the case of bacteria of the Burkholderia cepacia complex (Bcc). Bcc is a group of opportunistic pathogens with relatively large genomes that can cause lethal lung infections amongst cystic fibrosis (CF) patients. To characterise sRNAs expressed by Bcc bacteria when infecting a host, the nematode Caenorhabditis elegans was used as an infection model by the epidemic CF strain B. cenocepacia J2315. A total of 108 new and 31 previously described sRNAs with a predicted Rho independent terminator were identified, most of them located on chromosome 1. RIT11b, a sRNA downregulated under C. elegans infection conditions, was shown to directly affect B. cenocepacia virulence, biofilm formation, and swimming motility. RIT11b overexpression reduced the expression of the direct targets dusA and pyrC, involved in biofilm formation, epithelial cell adherence, and chronic infections in other organisms. The in vitro direct interaction of RIT11b with the dusA and pyrC messengers was demonstrated by electrophoretic mobility shift assays. To the best of our knowledge this is the first report on the functional characterization of a sRNA directly involved in B. cenocepacia virulence. Key points • 139 sRNAs expressed by B. cenocepacia during C. elegans infection were identified • The sRNA RIT11b affects B. cenocepacia virulence, biofilm formation, and motility • RIT11b directly binds to and regulates dusA and pyrC mRNAs

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Section: Discussionmentioning

confidence: 99%