The Function of Ac-Globulin in Blood Clotting

Ware, Arnold G.; Murphy, Robert C.; Seegers, Walter H.

doi:10.1126/science.106.2764.618-a

Cited by 94 publications

(20 citation statements)

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“…Quick (1943), prothrombin accelerator, Fantl and Nance (1946), factor V, Owren (1947), accelerator globulin. Ware, Murphy and Seegers (1947), bnt in this investigation is called accessory factor because of this multiplicity of names. It seems very probable that all these substances are one and the same.…”

Section: Origin Of Thronibin a And Thrombin Bmentioning

confidence: 99%

“…A serum factor is now recognized which has been named by various investigators according to their concept of how it best fits with their interpretation of the coagulation mechanism. Owren (1947) postulated factor VI, Ware and Seegers (1947) serum accelerator globulin, Jacox (1949) prothrombinconverting factor, de Vries et al (1949) serum prothrombin conversion accelerator (SPCA). Experiments were undertaken in an attempt to ascertain the relationship of thrombin A and thrombin B to the above factors.…”

Section: Origin Of Thronibin a And Thrombin Bmentioning

confidence: 99%

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Thrombin Components in Blood Coagulation

1951

Aust J Exp Biol Med

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It has been regarded as axiomatic in present day theories of blood coagulation that the action of thrombin upon fibrinogen is a simple one, and that thrombin itself is a unit substance. Lyons (.1945a, b) and Laki and Mommaerts (1945) suggested a two-stage process in the clotting of fibrinogen. Lyons produced evidence that tibrinogen went thi-ough a well-defined intermediate stage which he called fibrinogen B, before it was finally converted to fibrin. Lyons eiul. (1948), (.1949) also showed that this intermediate stage, fibrinogen B, could be demonstrated in certain pathological plasmas. In healthy normal subjects the incidence of fibrinogen B is very low. One hundred and five football players were examined both before and after play. Of these two showed a positive fibrinogen B reaction before play and the same players and one other previously negative gave a positive reaction after play.Since the two forms of fibrinogen were so sharply defined, five possibilities were considered as to their significance in the thrombin fibrinogen reaction.1. That the formation of fibrinogen B is not connected with the action of thrombin.2. That the transformation of fibrinogen to fibrin by thrombin could be inhibited at the stage of fibrinogen B by an anti-thrombin.3. That the thrombin molecule contains two active groups. 4. That thrombin could be composed of at least two components, thrombin A and thrombin B.5. That thrombin A and thrombin B are by-products of the thrombin fibrinogen reaction.Examing these possibilities in the order cited: Number 1 was untenable, as a fibrinogen B is always formed as an intermediate stage in the normal conversion of fibrinogen to fibrin. Whenever the fibrinogen B test is performed after one half of the known clotting time has elapsed, a maximal amount of fibrinogen B is demonstrated. As no fibrinogen can be detected after the removal of the gel formed by the test, it appears that all the fibrinogen had been transformed to fibrinogen B.iWork done under a grant from the National Health and Medical Eesearch Council.

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Section: Origin Of Thronibin a And Thrombin Bmentioning

confidence: 99%

Section: Origin Of Thronibin a And Thrombin Bmentioning

confidence: 99%

Thrombin Components in Blood Coagulation

1951

Aust J Exp Biol Med

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“…At first this retarded 1 Henry C. Buswell Research Fellow. conversion was thought to be related to denaturation of prothrombin attendant upon chemical procedures necessary for fractionation and purification. Later Ware et al (2,3) and Fantl and Nance (4) were able to produce acceleration of thrombin formation from this purified prothrombin fraction, when serum was added to the prothrombin, thromboplastin and calcium reaction mixture. The protein fraction responsible for accelerating prothrombin conversion was called accelerator globulin (ac-globulin).…”

mentioning

confidence: 99%

“…The protein fraction responsible for accelerating prothrombin conversion was called accelerator globulin (ac-globulin). Ware et al (3) believed that ac-globulin produced a reaction of co-autocatalysis, wherein thrombin accelerates its own formation through an intermediate action of acglobulin. A minute amount of thrombin produced early in coagulation of blood, liberates accelerator globulin which then intensifies the rate of thrombin formation from prothrombin.…”

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confidence: 99%