1996
DOI: 10.1016/0038-0717(95)00101-8
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The fumigation-extraction method to estimate soil microbial biomass: Calibration of the kEN value

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Cited by 750 publications
(284 citation statements)
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“…Total organic C and N in both fumigated and non-fumigated (control) soil extracts were analyzed using a CN analyzer (TOC-V CPH -TN Shimadzu). The values of nonfumigated samples were subtracted from those obtained from fumigated samples, and MBC and MBN were calculated using a K EC factor of 0.45 for MBC [30,31] and K EC factor of 0.54 for the MBN [32].…”
Section: Plant and Soil Sample Collection And Analysismentioning
confidence: 99%
“…Total organic C and N in both fumigated and non-fumigated (control) soil extracts were analyzed using a CN analyzer (TOC-V CPH -TN Shimadzu). The values of nonfumigated samples were subtracted from those obtained from fumigated samples, and MBC and MBN were calculated using a K EC factor of 0.45 for MBC [30,31] and K EC factor of 0.54 for the MBN [32].…”
Section: Plant and Soil Sample Collection And Analysismentioning
confidence: 99%
“…Characteristics of the investigated soils. Available P is presented by the quantity of isotopically exchangeable P within the first minute [5], available K by K extractable in saturated CO 2 water [1]; microbial C and N determined by chloroform fumigation [40] using k EC and k EN factors [13,14]. subsample for 24 h at 4 o C and 200 rpm in a water bath in order to remove microbial matter from truly undigested material [17].…”
Section: Manuresmentioning
confidence: 99%
“…When immobilization and mobilization processes of N in soil are managed, it is important to quantify the real amount of N stored in the soil microbial biomass (Joergensen and Mueller 1996). Transient immobilization of soil N in the microbial biomass may contribute to improved conservation of soil N sources (Jensen 1997).…”
Section: Decomposition Of Contrasting Leguminous Leaf Materials and Grmentioning
confidence: 99%