The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis

Cumplido-Laso, Guadalupe; Medina-Puche, Laura; Moyano, Enriqueta; Hoffmann, Thomas; Sinz, Quirin; Ring, Ludwig; Studart-Wittkowski, Claudia; Caballero, José L.; Schwab, Wilfried; Muñoz‐Blanco, Juan; Blanco‐Portales, Rosario

doi:10.1093/jxb/ers120

Cited by 113 publications

(94 citation statements)

References 58 publications

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“…In both climacteric and nonclimacteric fruits, water deficiency generated a rise in ABA content, which accelerated ripening and was accompanied by changes in both metabolites and gene expression (Castellarin et al, 2007;Gong et al, 2010). This dual regulation has also been observed for other genes whose expression is also up-regulated throughout the strawberry fruit-ripening process (Cumplido-Laso et al, 2012;Daminato et al, 2013;Molina-Hidalgo et al, 2013) and molecularly supported both the proposal of PerkinsVeazie (1995) and the findings described by Chai et al (2011), and Medina-Puche et al (2014. This hormonally regulated expression pattern was also similar to that observed for the FaMYB10 gene (MedinaPuche et al, 2014), and it strengthens the relationship between FaMYB10 and FaEOBII.…”

Section: Faeobii Expression Is Hormonally Regulated In a Similar Way mentioning

confidence: 70%

“…5). This expression model has been described for other receptacle-specific and ripening-related genes (Moyano et al, 1998;Blanco-Portales et al, 2002;Raab et al, 2006;Cumplido-Laso et al, 2012;Molina-Hidalgo et al, 2013). On the contrary, when ABA content in the receptacle was diminished by transient silencing of FaNCED1 expression or inhibition of the corresponding enzyme activity by the inhibitor NDGA, a decrease in FaEOBII expression was observed.…”

Section: Faeobii Expression Is Hormonally Regulated In a Similar Way mentioning

confidence: 71%

“…Supplemental Table S5 depicts the primer sequences used for quantitative amplification. Each reaction was performed at least in triplicate, and the corresponding C t values were normalized using the C t value corresponding to an interspacer 26S-18S strawberry RNA gene (Benítez-Burraco et al, 2003;Raab et al, 2006;Encinas-Villarejo et al, 2009;Cumplido-Laso et al, 2012;Molina-Hidalgo et al, 2013). All these values were then used to determine the relative increase or decrease of FaEOBII expression in the samples as compared with those in the control, in accordance with Pedersen (2001).…”

Section: Validation Of Microarray Data and Expression Analysis By Qrtmentioning

confidence: 99%

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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

et al. 2015

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Eugenol is a volatile phenylpropanoid that contributes to flower and ripe fruit scent. In ripe strawberry (Fragaria 3 ananassa) fruit receptacles, eugenol is biosynthesized by eugenol synthase (FaEGS2). However, the transcriptional regulation of this process is still unknown. We have identified and functionally characterized an R2R3 MYB transcription factor (EMISSION OF BENZENOID II [FaEOBII]) that seems to be the orthologous gene of PhEOBII from Petunia hybrida, which contributes to the regulation of eugenol biosynthesis in petals. The expression of FaEOBII was ripening related and fruit receptacle specific, although high expression values were also found in petals. This expression pattern of FaEOBII correlated with eugenol content in both fruit receptacle and petals. The expression of FaEOBII was repressed by auxins and activated by abscisic acid, in parallel to the ripening process. In ripe strawberry receptacles, where the expression of FaEOBII was silenced, the expression of CINNAMYL ALCOHOL DEHYDROGENASE1 and FaEGS2, two structural genes involved in eugenol production, was down-regulated. A subsequent decrease in eugenol content in ripe receptacles was also observed, confirming the involvement of FaEOBII in eugenol metabolism. Additionally, the expression of FaEOBII was under the control of FaMYB10, another R2R3 MYB transcription factor that regulates the early and late biosynthetic genes from the flavonoid/phenylpropanoid pathway. In parallel, the amount of eugenol in FaMYB10-silenced receptacles was also diminished. Taken together, these data indicate that FaEOBII plays a regulating role in the volatile phenylpropanoid pathway gene expression that gives rise to eugenol production in ripe strawberry receptacles.The octoploid cultivated strawberry (Fragaria 3 ananassa) is one of the most economically important, nonclimacteric, soft fruits, in which volatile compounds influence fruit flavor and aroma. Both characteristics contribute to the fruit organoleptic traits and are crucial factors to determine fruit quality.At present, extensive surveys on the components that contribute to strawberry flavor have been performed. In these studies, more than 360 volatiles have been identified (Latrasse, 1991;Nijssen, 1996;Zabetakis and Holden, 1997), but only 15 to 20 of them in wild varieties of strawberry are believed to be essential for sensory quality, together with nonvolatile sugars and organic acids (Schieberle and Hofmann, 1997). In contrast, in cultivated varieties of strawberry, only about six odor-active compounds have been identified as contributors to fruit flavor (Raab et al., 2006;Ulrich et al., 2007). Strawberry aroma is the result of the combined perception of fruity (ethyl butanoate, ethyl hexanoate, and methyl 2-methylbutanoat), green (Z-3-hexenal), sweaty (butanoic acid and 2-methylbutanoic acid), peach-like (g-decalactone), and caramel-like [4-hydroxy-2,5-dimethyl-3(2H)-furanone and 2,5-dimethyl-4-methoxy-3(2H)-furanone] flavor notes (Pyysalo et al., 1979;Larsen and Poll, 1992). Several wild strawb...

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Section: Faeobii Expression Is Hormonally Regulated In a Similar Way mentioning

confidence: 70%

Section: Faeobii Expression Is Hormonally Regulated In a Similar Way mentioning

confidence: 71%

Section: Validation Of Microarray Data and Expression Analysis By Qrtmentioning

confidence: 99%

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An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

et al. 2015

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“…Primers specific for actin (Chai et al, 2011;Sun et al, 2013) and the intergenic region 26S-18S (Cumplido-Laso et al, 2012) were used as internal standards. Data obtained were analyzed using the qPCR instrument software version 1.0 (LightCycler Nano System, 2011).…”

Section: Rna Isolation and Cdna Synthesismentioning

confidence: 99%

Effect of ethylene treatment on phytochemical and ethylene-related gene expression during ripening in strawberry fruit Fragaria x ananassa cv. Camino Real

et al. 2015

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ABSTRACT. In contrast to climacteric fruits, in which ethylene is known to be pivotal, the regulation of ripening in non-climacteric fruits is not well understood. The strawberry is a typical example of a non-climacteric fruit, which has been used as a model system of these types of fruit. In this study, the effect of exogenous ethephon on the expression of ethylene biosynthesis and signaling genes, FaERF2 and FaACO1, was analyzed in the Fragaria ananassa cultivar Camino Real by quantitative real-time polymerase chain reaction, and the physicochemical and phytochemical characteristics of fruits were evaluated in field trials and postharvest tests. Transcript accumulation was influenced by exogenous treatment with ethephon, which affected the pattern of gene expression during different stages of growth and fruit development, with the highest expression occurring during postharvest tests. In addition, ethephon significantly influenced the phytochemical profile of sugars, anthocyanins, phenolic compounds, and vitamin C contents both in the field-and postharvesttreated fruits at different stages. These results indicate that ethylene regulates the phenylpropanoid maturation pathway in strawberry fruit.

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“…fruit ripening (Aharoni et al, 2000;Cumplido-Laso et al, 2012), we analyzed the production of volatile esters by solid-phase microextraction gas chromatography (GC)-MS in RNAi-mediated CHSsilenced fruits and in fruits of the stable CHS antisense transgenic line. The relative content of ethyl 3-methylbutanoate was significantly increased by 2.2-fold in fruits after transient silencing of FaCHS, whereas levels of 2-methylbutanoic acid and methyl 2-methylpropanoate were significantly enhanced by 2.5-and 2.1-fold, respectively, in fruits of the stable transgenic line compared with the values of the wild-type fruits (Fig.…”

Section: Production Of Volatiles Is Enhanced In Transient and Stable mentioning

confidence: 99%

Acylphloroglucinol biosynthesis in strawberry fruit

et al. 2015

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ORCID IDs: 0000-0001-6609-7034 (J.S.); 0000-0002-9753-3967 (W.S.).Phenolics have health-promoting properties and are a major group of metabolites in fruit crops. Through reverse genetic analysis of the functions of four ripening-related genes in the octoploid strawberry (Fragaria 3 ananassa), we discovered four acylphloroglucinol (APG)-glucosides as native Fragaria spp. fruit metabolites whose levels were differently regulated in the transgenic fruits. The biosynthesis of the APG aglycones was investigated by examination of the enzymatic properties of three recombinant Fragaria vesca chalcone synthase (FvCHS) proteins. CHS is involved in anthocyanin biosynthesis during ripening. The F. vesca enzymes readily catalyzed the condensation of two intermediates in branched-chain amino acid metabolism, isovaleryl-Coenzyme A (CoA) and isobutyryl-CoA, with three molecules of malonyl-CoA to form phlorisovalerophenone and phlorisobutyrophenone, respectively, and formed naringenin chalcone when 4-coumaroyl-CoA was used as starter molecule. Isovaleryl-CoA was the preferred starter substrate of FvCHS2-1. Suppression of CHS activity in both transient and stable CHSsilenced fruit resulted in a substantial decrease of APG glucosides and anthocyanins and enhanced levels of volatiles derived from branched-chain amino acids. The proposed APG pathway was confirmed by feeding isotopically labeled amino acids. Thus, Fragaria spp. plants have the capacity to synthesize pharmaceutically important APGs using dual functional CHS/ (phloriso)valerophenone synthases that are expressed during fruit ripening. Duplication and adaptive evolution of CHS is the most probable scenario and might be generally applicable to other plants. The results highlight that important promiscuous gene function may be missed when annotation relies solely on in silico analysis.

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The fruit ripening-related gene FaAAT2 encodes an acyl transferase involved in strawberry aroma biogenesis

Cited by 113 publications

References 58 publications

An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

An R2R3-MYB Transcription Factor Regulates Eugenol Production in Ripe Strawberry Fruit Receptacles

Effect of ethylene treatment on phytochemical and ethylene-related gene expression during ripening in strawberry fruit Fragaria x ananassa cv. Camino Real

Acylphloroglucinol biosynthesis in strawberry fruit

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