We classify invariant complex structures on 6-dimensional nilmanifolds up to equivalence. As an application, the behaviour of the associated Frölicher sequence is studied as well as its relation to the existence of strongly Gauduchon metrics. We also show that the strongly Gauduchon property and the balanced property are not closed under holomorphic deformation.1 Example 5.8. Let us consider the Lie algebra h 5 with the real basis {e 1 , . . . , e 6 } described in Theorem 2.1. Let us consider the complex structure J 0,0 given by J 0,0 e 1 = −e 2 , J 0,0 e 3 = −2e 2 − e 4 , J 0,0 e 5 = −e 6 , J 0,0 e 2 = e 1 , J 0,0 e 4 = −2e 1 + e 3 , J 0,0 e 6 = e 5 .