The frequency, antifungal susceptibility and enzymatic profiles of Candida species in cases of onychomycosis infection

Sav, Hafıze; Barış, Ayşe; Turan, Deniz; Altinbaş, Rabiye; Sen, Sümeyye

doi:10.1016/j.micpath.2018.01.036

Cited by 13 publications

(14 citation statements)

References 26 publications

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“…The antifungal susceptibility pattern identified in this study indicated that all isolates were highly susceptible to AMB (100%) and VRC (100%). According to the present results, which are supported by previous studies, susceptibility to AMB and VRC was detected in C. albicans isolates 24,25 . In contrast, Pakshir et al reported that 43% of C. albicans isolates from patients with onychomycosis were resistant to VRC in Iran 26 .…”

Section: Discussionsupporting

confidence: 92%

“…According to our results, 61.42% of C. albicans isolates produced phospholipases. In a study conducted by Sav et al, the highest phospholipase activity was reported in C. albicans isolates from nail samples 25 . Ramos Lde et al reported that all C. albicans isolates (100%) from cutaneous candidiasis produced both aspartyl protease and phospholipase 31 .…”

Section: Figurementioning

confidence: 94%

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Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Mohammadi

Ghasemi

Familsatarian

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: The aim of this study was to evaluate some virulence factors in Candida albicans isolates from patients with onychomycosis and determine the correlation between these factors and the antifungal resistance profile. Methods: Seventy species of C. albicans were confirmed using polymerase chain reaction amplification of the HWP1 gene. According to the Clinical & Laboratory Standards Institute guidelines, the susceptibility profile of four antifungal agents was investigated, and the production of aspartyl protease, phospholipase, haemolysin, and biofilm was determined. The correlation between these profiles was also investigated. Results: The isolates indicated different levels of resistance and production of virulence factors. Significant correlations were observed between the minimum inhibitory concentration (MIC) of fluconazole/itraconazole and biofilm production, between phospholipase production and fluconazole/ itraconazole MIC, and between fluconazole MIC and hemolytic activity in C. albicans isolates. The results also showed significant correlations between phospholipase activity and biofilm production. Conclusions: Our findings will contribute to a better understanding of the pathogenesis of C. albicans and characterize the relationship between virulence factors and antifungal resistance, which may suggest new therapeutic strategies considering the possible involvement of the virulence mechanism in the effectiveness of treatment.

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Section: Discussionsupporting

confidence: 92%

Section: Figurementioning

confidence: 94%

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Mohammadi

Ghasemi

Familsatarian

et al. 2020

Rev. Soc. Bras. Med. Trop.

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“…From the remaining 15 references that describe a safety concern, 13 were related to human health (Al‐Tekreeti et al., ; Aslani et al., ; Charsizadeh et al., ,b,c; Jahanshiri et al., ; Nejat et al., ; Ortiz et al., ; Sav et al., ; Scapaticci et al., ; Siavoshi et al., ; Taverna et al., ; Wasilewska and Wroblewska, ); one to animal health (Dangarembizi et al., ) and four to antimicrobial resistance (Sav et al., ; Scapaticci et al., ; Sekyere and Asante, ; Taverna et al., ).…”

Section: Assessmentmentioning

confidence: 99%

“…Out of these 15 papers describing a safety concern for one or several QPS yeast species, 10 referred to Candida kefyr (teleomorph = Kluyveromyces marxianus ) (Al‐Tekreeti et al., ; Aslani et al., ; Charsizadeh et al., ,b,c; Jahanshiri et al., ; Nejat et al., ; Ortiz et al., ; Sav et al., ; Scapaticci et al., )), five to Saccharomyces cerevisiae of which one was identified as Saccharomyces boulardii (Aslani et al., ; Dangarembizi et al., ; Scapaticci et al., ; Sekyere and Asante, ; Wasilewska and Wroblewska, )), five to Candida famata (teleomorph = Debaryomyces hansenii ) (Kumari et al. (); (Mohamed et al., ; Rajkowska and Kunicka‐Styczyńska, ; Taverna et al., ; Vieira et al., ) and one to Hanseniaspora uvarum (Siavoshi et al., ).…”

Section: Assessmentmentioning

confidence: 99%

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 9: suitability of taxonomic units notified to EFSA until September 2018

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2019

EFS2

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The qualified presumption of safety (QPS) procedure was developed to provide a harmonised generic pre‐evaluation to support safety risk assessments of biological agents performed by EFSA's Scientific Panels. The taxonomic identity, body of knowledge, safety concerns and antimicrobial resistance were assessed. Safety concerns identified for a taxonomic unit are, where possible and reasonable in number, reflected by ‘qualifications’ which should be assessed at the strain level by the EFSA's Scientific Panels. During the current assessment, no new information was found that would change the previously recommended QPS taxonomic units and their qualifications. Between April and September 2018, the QPS notification list was updated with 48 microorganisms from applications for market authorisation. Of these, 30 biological agents already had QPS status, 15 were excluded from the QPS exercise by the previous QPS mandate (five filamentous fungi) or from further evaluations within the current mandate (two notifications of Enterococcus faecium, one of Streptomyces spp. and seven of Escherichia coli). One taxonomic unit was (re)evaluated: Pseudomonas fluorescens had been previously evaluated in 2016, and was now re‐evaluated within this mandate. The revision of the literature supports the previously identified safety concerns (e.g. production of biocompounds with antimicrobial activity and virulence features), preventing the inclusion of P. fluorescens in the QPS list. Mycobacterium setense and Komagataeibacter sucrofermentans were evaluated for the first time. M. setense cannot be considered for the QPS assessment because there are significant safety concerns. K. sucrofermentans (Acetobacter xylinus subsp. sucrofermentans) can be proposed for the QPS list but only for production purposes. The QPS status of Corynebacterium glutamicum is confirmed with the qualification extended to other production purposes.

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“…[1][2][3] Non-albicans Candida türlerinden Candida parapsilosis (C.parapsilosis), tüm dünyada yaygın olarak özellikle el tırnaklarını etkileyen onikomikozdan başlayarak, ölümle sonuçlanabilen yaygın enfeksiyonlara veya invaziv kandidiyazise yol açabilmektedir. 4 C. parapsilosis yenidoğan yoğun bakım ünitelerinde meydana gelen kandidemi sonucu geç sepsise ve böylece yüksek mortalite ve morbiditeye yol açmaktadır. 5 Özellikle kandidemi, C. parapsilosis'in biyofilm oluşturma ve total parenteral nutrisyon gibi zengin içerikli solüsyonlarda çoğalması ile yakından ilişkilidir.…”

unclassified

Klinik Örneklerden İzole Edilmiş Candi̇da parapsi̇losi̇s Suşlarının Bi̇yofi̇lm Oluşturma, Hemoli̇ti̇k ve Koagülaz Akti̇vi̇teleri̇ ile Anti̇fungal Duyarlılıklarının Karşılaştırılması

Keçeli

Kurt

Özgür

et al. 2020

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

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Candida parapsilosis suşlarının in vitro biyofilm oluşturma, hemoliz ve koagülaz aktiviteleri gibi virülans faktörlerinin araştırılması ve antifungal duyarlılıklarıyla karşılaştırılması amaçlanmıştır. öntem: Kocaeli Üniversitesi'nden 26, Mersin Üniversitesi'nden 14 suş çalışmaya alınmıştır. Hemolitik aktivite %3 glikozlu koyun kanlı agarda 37 o C'de 48 saatlik, koagülaz aktivite tüp test metodu ile 37 o C'de 4 saatlik, biyofilm oluşumu ise modifiye Christensen makro tüp metodu ile %8 glikozlu Sabouraud Dekstroz sıvı besiyerinde 37 o C'de 48-72 saatlik inkübasyon sonrasında değerlendirilerek belirlenmiştir. Flukonazol, kaspofungin, mikafungin, vorikonazol ve amfoterisin B'ye karşı MIK değerleri VİTEK-2 otomatize sistem ile belirlenmiştir. Bulgular: Suşların 23'ü periferik kandan, 7'si damar içi kateterden, 3'er adet periton ve idrardan, 2 adet tırnak örneğinden, 1'er adet plevra ve balgam örneklerinden izole edilmiştir. Biyofilm aktivitesi tüm suşların 32'sinde (%80) saptamış olup, 13'ü (%32,5) zayıf, 12'si (%30) orta, 7'si (%17,5) ise güçlü pozitif olarak skorlanmıştır. Suşların yarısı alfa hemoliz, 13'ü (%32,5) beta hemoliz gösterirken, 7'sinde (%17,5) hemoliz saptanmamıştır. Koagülaz aktivitesi tüm suşlarda negatif bulunmuştur. Tüm suşlar vorikonazol ve amfoterisin B'ye duyarlı saptanmıştır. Biyofilm aktivitesi zayıf olan iki suşta flukonazol direncine rastlanmıştır. Periferik kandan izole edilmiş 7 suş ile 2'si periton ve biri idrar suşu olmak üzere toplam 10 suşta (%25) kaspofungin direnci saptanmıştır. Bu suşların 4'ünde aynı zamanda mikafungin direnci, 7'sinde hemolitik aktivite ve biyofilm oluşumu gözlenmiştir. Antifungal direnci ile virülans faktörleri arasında istatistiksel olarak anlamlı bir ilişki bulunamamıştır. Sonuç: Biyofilm oluşturma ve hemolitik aktivite C. parapsilosis'in başlıca virülans faktörleridir ve sıklıkla bu faktörlere bağlı olarak kandidemi ve kateter enfeksiyonları oluşabilmektedir. Bu enfeksiyonların tedavi protokolleri epidemiyolojik ve antifungal duyarlılık paterni verilerine göre değişim gösterebilir. Daha fazla C. parapsilosis suşu kullanarak ileri çalışmalar yapılması önerilmektedir.

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The frequency, antifungal susceptibility and enzymatic profiles of Candida species in cases of onychomycosis infection

Cited by 13 publications

References 26 publications

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Relationship between antifungal susceptibility profile and virulence factors in Candida albicans isolated from nail specimens

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 9: suitability of taxonomic units notified to EFSA until September 2018

Klinik Örneklerden İzole Edilmiş Candi̇da parapsi̇losi̇s Suşlarının Bi̇yofi̇lm Oluşturma, Hemoli̇ti̇k ve Koagülaz Akti̇vi̇teleri̇ ile Anti̇fungal Duyarlılıklarının Karşılaştırılması

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