The fractionation of phosphate esters on ion-exchange resin by a new system of pH-gradient elution

Quantitation of ribonucleotides from base-hydrolyzed RNA using capillary zone electrophoresis

“…Toward these ends, several methods of molecular discrimination have been previously employed to separate nucleotides acquired from complete hydrolysis of RNA. These include paper electrophoresis (1), ion-exchange chromatography (2), high-performance liquid chromatography (HPLC) (3), and isotachophoresis (4). Recently, capillary zone electrophoresis (CZE) has been shown to be applicable to the problem of nucleotide and oligonucleotide separation (5)(6)(7).…”

The Turnover of Ribonucleic Acids Their Degradation by Characteristic Enzymic Pathways in Escherichia Coli

“…Extracts from cells in the phosphorus deficient state had abnormally low contents of nucleoside di-and triphosphates and showed a rather specific increase in monophosphates and nucleosides. Subsequent analyses of the monophosphate fractions by rechromatography on Dowex 1 (x-8, f ormate) with a linear pH-gradient (Wade, 1960;Maruyama and Mizuno, 1965) showed that they contain as characteristic nucleotides the four main ribonucleoside 3'-monophosphates and the three ribonucleoside 2', 3'-cyclic phosphates other than that with the guanine moiety (Maruyama and Mizuno, 1966). These compounds are not found in the cell pool under normal conditions.…”