The Fos expression in rat brain following electrical stimulation of dura mater surrounding the superior sagittal sinus changed with the pre-treatment of rizatriptan benzoate

Wang, Xiaolin; Yu, Shengyuan; Dong, Zhao; Jiang, Lei

doi:10.1016/j.brainres.2010.10.004

Cited by 14 publications

(7 citation statements)

References 31 publications

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“…In agreement with previous studies, 25 28 our results showed significant increases in c-Fos immunoreactivity within the PAG, RMg, and TNC after repeated dural stimulation, supporting the notion that activation of the PAG-RMg pathway in the descending pain modulatory system contributes to migraine pathophysiology through impaired descending inhibition and/or enhanced descending facilitation. 22 Pretreatment with EA strikingly decreased c-Fos immunoreactivity within these structures.…”

Section: Discussionsupporting

confidence: 93%

Effect of Electroacupuncture Pretreatment at Gb20 on Behaviour and the Descending Pain Modulatory System in a Rat Model of Migraine

et al. 2016

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BackgroundWhile electroacupuncture (EA) pretreatment has been found to ameliorate migraine-like symptoms, the underlying mechanisms remain poorly understood. Emerging evidence suggests that the brainstem descending pain modulatory system, comprising the periaqueductal grey (PAG), raphe magnus nucleus (RMg), and trigeminal nucleus caudalis (TNC), may be involved in migraine pathophysiology. We hypothesised that EA would ameliorate migraine-like symptoms via modulation of this descending system.MethodsWe used a conscious rat model of migraine induced by repeated electrical stimulation of the dura. Forty male Sprague-Dawley rats were randomly assigned to one of four groups: an EA group, which received EA at GB20 following dural stimulation; a sham acupuncture (SA) group, which received manual acupuncture at a non-acupuncture point following dural stimulation; a Model group, which received dural stimulation but no acupuncture; and a Control group, which received neither dural stimulation nor acupuncture (electrode implantation only). HomeCageScan was used to measure effects on behaviour, and immunofluorescence staining was used to examine neural activation (c-Fos immunoreactivity) in the PAG, RMg, and TNC.ResultsCompared to the Model group, rats in the EA group showed a significant increase in exploratory, locomotor and eating/drinking behaviour (p<0.01) and a significant decrease in freezing-like resting and grooming behaviour (p<0.05). There was a significant increase in the mean number of c-Fos neurons in the PAG, RMg, and TNC in Model versus Control groups (p<0.001); however, this was significantly attenuated by EA treatment (p<0.001). There were no significant differences between the SA and Model groups in behaviour or c-Fos immunoreactivity.ConclusionsEA pretreatment ameliorates behavioural changes in a rat model of recurrent migraine, possibly via modulation of the brainstem descending pathways.

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Section: Discussionsupporting

confidence: 93%

Effect of Electroacupuncture Pretreatment at Gb20 on Behaviour and the Descending Pain Modulatory System in a Rat Model of Migraine

et al. 2016

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“…Atypical rs-FC between the PAG and cerebral regions such as the anterior insula and amygdala in migraine patients may indicate that pain modulation is more sophisticated in humans than in our rat model of recurrent headache. Previous studies have demonstrated that other brainstem regions such as the dorsal rostral pons 59 and the TNC 60 , play important roles in the pathogenesis of migraine. Among clinical studies, only one study revealed that migraineurs with severe allodynia have stronger PAG rs-FC to other brainstem regions including the pons and ventral medulla 25 .…”

Section: Discussionmentioning

confidence: 99%

Disrupted functional connectivity between the periaqueductal gray and other brain regions in a rat model of recurrent headache

Jia

Tang

Zhao

et al. 2017

Sci Rep

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Functional connectivity (FC) has been used to investigate the pathophysiology of migraine. We aimed to identify atypical FC between the periaqueductal gray (PAG) and other brain areas in rats induced by repeated meningeal nociception. The rat model was established by infusing an inflammatory soup (IS) through supradural catheters in conscious rats. Quiescent and face-grooming behaviors were observed to assess nociceptive behavior. FC analysis seeded on the PAG was performed on rats 21 days after IS infusion. The rats exhibited nociceptive behavior correlates of human behaviors associated with migraine after IS infusion. The PAG showed increased FC with the prefrontal cortex, cingulate gyrus, and motor cortex but decreased FC with the basal ganglia, dorsal lateral thalamus, internal capsule and prelimbic cortex in the rat model. The atypical FC of the PAG with brain regions in the rat model that are involved in nociception, somatosensory processing, emotional processing, and pain modulation are consistent with the clinical data from migraineurs, indicate that resting-state FC changes in migraine patients may be a consequence of headache attacks, and further validate this rat model of chronic migraine.

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“…Various types of noxious stimulation, including thermal, mechanical and chemical stimuli, induce the expression of Fos in the brain and spinal cord (Han et al ., ). A previous study from our group (Wang et al ., ) demonstrated that Fos‐ir neurons have comparatively more dense distributions in the TNC, RVM and PAG than in other brain regions in an animal model of migraine, which is why these brain regions were assessed for Fos‐ir neurons in this study. The frontal cortex was also assessed in this study because of its possible role in headache modulation (Bongsebandhu‐phubhakdi & Srikiatkhachorn, ).…”

Section: Discussionmentioning

confidence: 92%

Rizatriptan overuse promotes hyperalgesia induced by dural inflammatory stimulation in rats by modulation of the serotonin system

Min

Han

et al. 2016

Eur J of Neuroscience

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Clinical and preclinical studies have implicated serotonin (5-HT) and the 5-HT2A receptor (5-HT2AR) in the pathogenesis of medication-overuse headache (MOH). However, with no appropriate animal model to study this phenomenon it is difficult to differentiate the effects of chronic exposure to analgesics from the consequences of frequent headache attacks during the development of MOH. Therefore, this study used a novel animal model of MOH established by a combination of the overuse of rizatriptan (RIZ) and stimulation with dural inflammatory soup (IS) to investigate whether 5-HT and 5-HT2AR are involved in central plasticity and hyperalgesia. Similar to an IS infusion, IS-RIZ treatment induced nociception-related behaviours in Sprague-Dawley rats and increased Fos expression in the cortex and trigeminal pathway, whereas the RIZ injection alone did not. In addition, overuse of RIZ, administration of an IS stimulus, and a combination of these treatments, decreased the periorbital withdrawal threshold, with IS-RIZ treatment having the most significant effects. Both chronic RIZ exposure and recurring nociception decreased 5-HT expression, whereas IS-RIZ treatment led to decreased expression of 5-HT and upregulation of 5-HT2AR, which was positively correlated with Fos activation. These findings suggest that overuse of RIZ does not directly induce pain via the activation of nociceptive pathways but may increase hyperalgesia by influencing the pain modulation system. Furthermore, decreased 5-HT levels and upregulation of 5-HT2AR may play important roles in this system. Taken together, these findings indicate that medication overuse and frequent headache attacks can promote the neural plasticity associated with MOH.

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The Fos expression in rat brain following electrical stimulation of dura mater surrounding the superior sagittal sinus changed with the pre-treatment of rizatriptan benzoate

Cited by 14 publications

References 31 publications

Effect of Electroacupuncture Pretreatment at Gb20 on Behaviour and the Descending Pain Modulatory System in a Rat Model of Migraine

Effect of Electroacupuncture Pretreatment at Gb20 on Behaviour and the Descending Pain Modulatory System in a Rat Model of Migraine

Disrupted functional connectivity between the periaqueductal gray and other brain regions in a rat model of recurrent headache

Rizatriptan overuse promotes hyperalgesia induced by dural inflammatory stimulation in rats by modulation of the serotonin system

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