The formulation, characterization and<i>in vivo</i>evaluation of a magnetic carrier for brain delivery of NIR dye

Raut, Sangram; Kirthivasan, Bharat; Bommana, Murali Mohan; Squillante, Emilio; Sadoqi, Mostafa

doi:10.1088/0957-4484/21/39/395102

Cited by 31 publications

(26 citation statements)

References 33 publications

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“…59 This advanced drug targeting approach is increasingly utilized due to increased site specificity with fewer side effects. 26 However, efficiency of magnetic field-mediated cellular delivery of drug depends on the applied external magnetic field as well as the type of MNP carrier.…”

Section: Magnetic Targetingmentioning

confidence: 99%

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Yallapu¹,

Othman²,

Curtis³

et al. 2012

IJN

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Background:The next generation magnetic nanoparticles (MNPs) with theranostic applications have attracted significant attention and will greatly improve nanomedicine in cancer therapeutics. Such novel MNP formulations must have ultra-low particle size, high inherent magnetic properties, effective imaging, drug targeting, and drug delivery properties. To achieve these characteristic properties, a curcumin-loaded MNP (MNP-CUR) formulation was developed. Methods: MNPs were prepared by chemical precipitation method and loaded with curcumin (CUR) using diffusion method. The physicochemical properties of MNP-CUR were characterized using dynamic light scattering, transmission electron microscopy, and spectroscopy. The internalization of MNP-CUR was achieved after 6 hours incubation with MDA-MB-231 breast cancer cells. The anticancer potential was evaluated by a tetrazolium-based dye and colony formation assays. Further, to prove MNP-CUR results in superior therapeutic effects over CUR, the mitochondrial membrane potential integrity and reactive oxygen species generation were determined. Magnetic resonance imaging capability and magnetic targeting property were also evaluated. Results: MNP-CUR exhibited individual particle grain size of ∼9 nm and hydrodynamic average aggregative particle size of ∼123 nm. Internalized MNP-CUR showed a preferential uptake in MDA-MB-231 cells in a concentration-dependent manner and demonstrated accumulation throughout the cell, which indicates that particles are not attached on the cell surface but internalized through endocytosis. MNP-CUR displayed strong anticancer properties compared to free CUR. MNP-CUR also amplified loss of potential integrity and generation of reactive oxygen species upon treatment compared to free CUR. Furthermore, MNP-CUR exhibited superior magnetic resonance imaging characteristics and significantly increased the targeting capability of CUR. Conclusion: MNP-CUR exhibits potent anticancer activity along with imaging and magnetic targeting capabilities. This approach can be extended to preclinical and clinical use and may have importance in cancer treatment and cancer imaging in the future. Further, if these nanoparticles can functionalize with antibody/ligands, they will serve as novel platforms for multiple biomedical applications.

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Section: Magnetic Targetingmentioning

confidence: 99%

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Yallapu¹,

Othman²,

Curtis³

et al. 2012

IJN

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“…This resulted in a safe conservative estimate of the brain content of MH. 19) Table 4 depicts the concentration of MH in heart, liver, kidney, spleen and lungs following administration of pure MH, MHNP and cMHNP. The concentration of MH in plasma was found to be higher in MHNP treated animals than pure MH treated group, which was further increased when treated with cMHNP (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…After 30 min, the sample was extracted and analyzed with the same procedure described as above. 19) Pharmacodynamic Study. Study Design The experimental animals (male swiss albino mice) were randomly divided in following groups having 6 mice in each group:…”

mentioning

confidence: 99%

Formulation, Optimization, <i>in Vivo</i> Pharmacokinetic, Behavioral and Biochemical Estimations of Minocycline Loaded Chitosan Nanoparticles for Enhanced Brain Uptake

Nagpal

Singh

Mishra

2013

CHEMICAL & PHARMACEUTICAL BULLETIN

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The minocycline hydrochloride (MH), at higher doses, is useful in the treatment of neurodegenerative disorders and owing to its antioxidant potential, it may have nootropic effects. MH loaded nanoparticles (MHNP) were coated with tween 80 (cMHNP) to improve its brain uptake followed by their optimization employing two factor-three level (3 2 ) central composite design (CCD) in order to minimize particle size and maximize drug entrapment efficiency (DEE) and validated. The optimized formulations were further subjected to in vitro drug release study; in vivo biodistribution studies in male wistar rats. The pharmacodynamic study was carried out using elevated plus maze (EPM) and Morris water maze (MWM) behavioral models for nootropic activity in swiss albino mice; and biochemical estimations (acetylcholine esterase, reduced glutathione, malondialdehyde and brain nitrite level). After intravenous (i.v.) administration, the concentration of MH in brain of cMHNP (6.21±0.64 µg/mL) treated rats was significantly higher with MH solution treated (0.70±0.06 µg/mL) as well as MHNP (1.03±0.12 µg/mL) treated animals. Pharmacodynamic studies revealed a significant improvement in memory of MH, MHNP and cMHNP treated swiss albino mice than saline treated control group. However, cMHNP revealed maximum decrease in transfer latency (TL) in EPM and maximum increase in time spent in target quadrant (TSTQ) in MWM. Although cMHNP did not produce significant change in brain acetylcholinesterase, but, significantly increased reduced glutathione, malondialdehyde and reduced brain nitrite level as compared to saline, MH solution and MHNP treated groups. The results suggest that cMHNP is a promising candidate for improved brain uptake of MH with better no o tro pic effect.

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“…Preparation of sPIONs SPION nanoparticles were prepared as described by Raut et al 36 Thermal decomposition was used to prepare oleic acid-coated magnetic nanoparticles (OAMNP). A total of 10 mL of octyl ether was added to 1.43 mL of oleic acid.…”

Section: Methodsmentioning

confidence: 99%

Superparamagnetic reconstituted high-density lipoprotein nanocarriers for magnetically guided drug delivery

Sabnis¹,

Sabnis²,

Raut³

et al. 2017

IJN

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Current cancer chemotherapy is frequently associated with short- and long-term side effects, affecting the quality of life of cancer survivors. Because malignant cells are known to overexpress specific surface antigens, including receptors, targeted drug delivery is often utilized to reduce or overcome side effects. The current study involves a novel targeting approach using specifically designed nanoparticles, including encapsulation of the anti-cancer drug valrubicin into superparamagnetic iron oxide nanoparticle (SPION) containing reconstituted high-density lipoprotein (rHDL) nanoparticles. Specifically, rHDL–SPION–valrubicin hybrid nanoparticles were assembled and characterized with respect to their physical and chemical properties, drug entrapment efficiency and receptor-mediated release of the drug valrubicin from the nanoparticles to prostate cancer (PC-3) cells. Prussian blue staining was used to assess nanoparticle movement in a magnetic field. Measurements of cytotoxicity toward PC-3 cells showed that rHDL–SPION–valrubicin nanoparticles were up to 4.6 and 31 times more effective at the respective valrubicin concentrations of 42.4 µg/mL and 85 µg/mL than the drug valrubicin alone. These studies showed, for the first time, that lipoprotein drug delivery enhanced via magnetic targeting could be an effective chemotherapeutic strategy for prostate cancer.

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The formulation, characterization andin vivoevaluation of a magnetic carrier for brain delivery of NIR dye

Cited by 31 publications

References 33 publications

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Curcumin-loaded magnetic nanoparticles for breast cancer therapeutics and imaging applications

Formulation, Optimization, <i>in Vivo</i> Pharmacokinetic, Behavioral and Biochemical Estimations of Minocycline Loaded Chitosan Nanoparticles for Enhanced Brain Uptake

Superparamagnetic reconstituted high-density lipoprotein nanocarriers for magnetically guided drug delivery

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