The formation of tigroid substance during postnatal maturation of the brain of mice after pre- and perinatal X-irradiation

Abstract

Using Nissl stained slices of postnatal brain, tigroid formation in neurons of the cortex, thalamus, cerebellum, hippocampus, gyrus dentatus and nucleus mot. trigemini was examined in X-irradiated mice. Following exposure on days 13, 16, 18.5 or 22 post conception with doses ranging from 0.5 Gy to 3.0 Gy tigroid formation was studied by means of a selective microphotometrical measurement technique. After irradiation, a fluctuating diminution in the tigroid density was observed in relation to the controls. It was dependent both on the dose and on the stage of development during exposure. In several brain regions fluctuating tigroid responses, being most pronounced during the "critical periods" of postnatal brain maturation, resulted in a longterm compensation of a deficit in the tigroid density after irradiation with 0.05 Gy. After the higher doses the density diminution was either not compensated or was progressive. The late tigroid responses decrease from irradiation on day 13 p.c. to irradiation day 22 p.c. Hence, this type of late maturation impairment was either extended through several cell generations or it was induced to a lesser degree in the early postmitotic neurons. Changes in the total RNA-content of the brain are concomitant with the range of the tigroid responses during the second and the beginning of the third week after birth. The tigroid reactions were interpreted as a chain of interdependent processes of retardation and stabilization. Accordingly, to obtain a better understanding of long-term maturation defects, a comprehensive evaluation of the whole chain of events will be required.

Key Developments in the Potential of Curcumin for the Treatment of Peripheral Neuropathies

“…While CIPN primary targets mainly involve peripheral nerves, recent data suggest direct toxicity in spinal cord neurons [ 82 ]. The functional state of neuronal cells can be assessed by formational changes in the Nissl bodies [ 83 ]. Spinal cord analysis of rats subjected to oxaliplatin showed curcumin-induced repairs injury in the spinal dorsal horn.…”

Biological effects after prenatal irradiation (embryo and fetus)

“…The Nissl bodies, units of the ergastoplasma with adjacent ribosomes, represent the light microscopic equivalent of the rough endoplasmatic reticulum. Computer conducted microscanning photometry with a spatial resolution of less than 1 mm was applied by Konermann and Schwald (1980) in investigations on tigroid density changes in representative neuronal groups of different brain areas (cortex, thalamus, cerebellum, hippocampus dentate gyrus, and nucleus motorius trigemini). Mice were irradiated with acute x-ray doses between 0.5 and 3.0 Gy on E13, E16, E18.5, or P3, and measurements were extended from neonatal stages to adulthood.…”