The formation of synapses in regenerating mammalian striated muscle

Bennett, Max R.; Florin, Todd; Woog, R.

doi:10.1113/jphysiol.1974.sp010511

Cited by 83 publications

(39 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…to increase in quantal content for several weeks (Bennett, Pettigrew & Taylor, 1973;Bennett, McLachlan & Taylor, 1973;Bennett et al 1974). Thus p increases to nearly 1P0 in just a few days at newly formed junctions, although the quantal content of the e.p.p.…”

Section: Resultsmentioning

confidence: 96%

“…A general description of transmission at junctions during synapse formation in reinnervated (Bennett, McLachlan & Taylor, 1973), and in regenerating mammalian striated muscle (Bennett et al 1974) Transmitter release by a single nerve impulse…”

Section: Resultsmentioning

confidence: 99%

“…All experiments were performed on junctions in regenerating or reinnervated soleus and extensor digitorum longus muscles of the rat, which were prepared and studied with conventional electrophysiological techniques, as described previously (Bennett, Florin & Woog, 1974;Bennett, McLachlan & Taylor, 1973).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

A statistical analysis of the release of acetylcholine at newly formed synapses in striated muscle

Bennett¹,

Florin²

1974

The Journal of Physiology

Self Cite

108

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A statistical analysis of the release of acetylcholine at newly formed synapses in striated muscle

Bennett¹,

Florin²

1974

The Journal of Physiology

Self Cite

108

View full text Add to dashboard Cite

show abstract

“…For studies of newly formed synapses, reinnervated rat soleus and extensor digitorum longus muscles were prepared in the manner previously described (Bennett, McLachlan & Taylor, 1973;Bennett, Florin & Woog, 1974 (Hubbard, 1963), and the combinations of [Ca] (Martin. 1955) were required.…”

Section: Methodsmentioning

confidence: 99%

The effect of calcium ions on the binomial statistic parameters which control acetylcholine release at synapses in striated muscle.

Bennett¹,

Florin²,

Hall³

1975

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. A study has been made of the effects of changing [Ca]. and [Mg]. on the binomial statistic parameters p and n which control the average quantal content (mi) of the synaptic potential due to acetylcholine release. 4. The facilitated increase in m9 during a short train was due to an increase in n, whereas the post-tetanic increase in mi during a tetanus was due to an increase in p. These results are considered in terms of the role of Ca ions in facilitation and post-tetanic potentiation.

show abstract

“…It has been established that synapses are formed in the regenerated muscle of amphibians (29, 37) and mammals (1,3,10). Marshall et al .…”

mentioning

confidence: 99%

Density and distribution of α-bungarotoxin-binding sites in postsynaptic structures of regenerated rat skeletal muscle

Bader¹

1981

The Journal of Cell Biology

View full text Add to dashboard Cite

Acetylcholine receptors (AChR) are organized in a discrete and predictable fashion in the postsynaptic regions of vertebrate skeletal muscle . When muscle is damaged, nerves and myofibers including muscular elements of the endplate degenerate, but the connective tissue elements survive. Muscle fibers regenerate within the basal lamina of the original myofiber . Postsynaptic differentiation in regenerated mammalian skeletal muscle can occur in different ways : (a) at the site of the original endplate in the presence or absence of the nerve, or (b) at ectopic regions of the regenerated myofiber in the presence of the nerve when the original endplate is not present. The present study used '251-a-bungarotoxin (1251 -a-BuTX) and EM autoradiography to examine the density and distribution of AChR in postsynaptic structures regenerated at the site of the original endplate in the absence of the nerve and at ectopic sites of the myofiber in the presence of the nerve when the original endplate was removed . In regenerated myofibers, the density of a-BuTX-binding sites fell within the range of densities observed in uninjured muscle whether postsynaptic differentiation occurred at the site of the original endplate in the absence of the nerve or at an originally ectopic position of the regenerated myofiber . In addition, the distribution of a-BuTX-binding sites within the regenerated postsynaptic regions closely resembled the distribution of a-BuTX-binding sites in uninjured muscle . Morphometric analysis was performed on postsynaptic structures formed at the site of the original endplate in the absence of the nerve or at an ectopic position of the regenerated myofiber by interaction of the nerve and muscle . Although variation in the depth of the primary cleft occurred, there was little difference between the overall structure of regenerated postsynaptic structures and that of endplates of uninjured muscles.The density of acetylcholine receptors (AChR) at innervated motor endplates of skeletal muscle has been determined by both physiological (23, 25) and morphological (16,28,30,(33)(34)(35) means. Various studies (for review see reference 12) have shown that the number of AChR at endplates in mice (2), rats (14, 31), frogs (31), and humans (13) is quite similar. Electron microscope autoradiographic studies have demonstrated a relatively constant density (16,28,30,(33)(34)(35) and distribution (16,(33)(34)(35) of AChR at endplates of vertebrate skeletal muscle .When skeletal muscle grafts are made, the neuromuscular junctions are disrupted by the degeneration of the myofibers and the nerves that supply them (7,10,29,37, and footnote 1).' Carlson, B. M., P. Hnik, S. Tucek, R. Vejsada, and D. Bader. 338It has been established that synapses are formed in the regenerated muscle of amphibians (29, 37) and mammals (1,3,10). Marshall et al . (29) have shown that, in regenerating frog muscle, neuromuscular connections are almost always at the site of the original endplate . Burden et al . (7) have demonstrated that postsyna...

show abstract

The formation of synapses in regenerating mammalian striated muscle

Cited by 83 publications

References 59 publications

A statistical analysis of the release of acetylcholine at newly formed synapses in striated muscle

A statistical analysis of the release of acetylcholine at newly formed synapses in striated muscle

The effect of calcium ions on the binomial statistic parameters which control acetylcholine release at synapses in striated muscle.

Density and distribution of α-bungarotoxin-binding sites in postsynaptic structures of regenerated rat skeletal muscle

Contact Info

Product

Resources

About