The formation of lipid bilayers on surfaces

Gromelski, Sandra; Saraiva, Ana M.; Krastev, Rumen; Brezesinski, Gerald

doi:10.1016/j.colsurfb.2009.08.006

Cited by 22 publications

(22 citation statements)

References 22 publications

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“…Another detailed study of the mechanics of lipid bilayer formation via vesicle adsorption was carried out by Gromelski et al who used QCM to show that depending on the chemical structure of the lipids, two different pathways are followed on SiO 2 surfaces: (i) vesicle adsorption occurs in a first step until a critical coverage is reached, followed by vesicle rupture and bilayer formation, or (ii) adsorption and vesicle rupture occur almost simultaneously (Gromelski et al , 2009). In the case of polyelectrolyte cushions, both neutron reflectivity and quartz crystal microbalance experiments showed that the formation of homogeneous DMPC bilayers was significantly better on the negatively charged PSS surface compared to the positively charged PAH surface.…”

Section: Lipids and Membranesmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

161

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Since the publication of the original review of piezoelectric acoustic sensors in this series there has been a consistent, gradual expansion in the number of published papers using 'quartz crystal microbalances' (QCM). Between 2001 and 2009, the number of QCM publications per annum has increased from 49 to 273, with a two-fold increase in papers per annum between 2004 and 2008. Within the field, comparing the time covered by the current to the previous review, there are trends towards increasing use of QCM in the study of protein adsorption to surfaces (93% increase), homeostasis (67% increase), protein-protein interactions (40% increase) and carbohydrates (43% increase). New commercial systems have been released that are driving the uptake of the technology for characterization of binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This paper highlights theoretical and practical aspects of the principles that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and membrane interfaces.

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Section: Lipids and Membranesmentioning

confidence: 99%

A survey of the 2006–2009 quartz crystal microbalance biosensor literature

Becker

Cooper

2011

J of Molecular Recognition

161

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“…This is for instance the case when the polar head groups are only involved in an interaction with an extrinsic protein [10]. At last, planar membrane models are particularly well-adapted to surface analysis techniques, such as Atomic Force Microscopy (AFM) or fluorescence microscopy as imaging techniques [11][12][13][14] or other methods such as quartz crystal microbalance [6,15,16].…”

Section: Introductionmentioning

confidence: 99%

Oxidation of Langmuir-Blodgett films of monounsaturated lipids studied by atomic force microscopy

Faye

Moroté

Grauby–Heywang

et al. 2013

IJNT

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International audienceIn this work, we studied the stability in time of Langmuir-Blodgett films of POPC and OPPC, two unsaturated phospholipids with similar chains, differing by the relative position of these chains on the glycerol backbone. These films, transferred from the air-water interface onto freshly cleaved mica, were characterised by Atomic Force Microscopy (AFM) giving information on their topography at a lateral and perpendicular resolution in the nm range. AFM images (obtained in tapping mode) of freshly transferred films are homogenous, in agreement with the fact that these two lipids are in a liquid-expanded phase under our experimental conditions. After two days, small domains are observed, higher than the surrounding phase of about 0.8 nm in both types of samples. These domains are not observed if the samples are kept under vacuum, or if LB films are made of saturated phospholipids, suggesting that they are due to the local oxidation of POPC or OPPC, the oxidation being slightly more pronounced in the last case. Their dispersion in LB films suggests that oxidation occurs at different points at the same time, likely in areas presenting a loose packing or a defect. The local increase of thickness could be due to the reversal of the oxidised chain, raising the oxidised lipid above the surrounding phase

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“…The Sauerbrey relationship can be used only when the difference between dissipation values for measured overtones does not exceed 10 −6 . In other cases the viscoelastic models of the film need to be used to evaluate its properties as the dissipation increment (ΔD) is related to viscoelastic properties of adsorbed film [38]. The QCM-D technique provides valuable real-time information about the mass and structural properties of adsorbed lipids [50,51].…”

Section: Dissipation-enhanced Quartz Crystal Microbalance (Qcm-d) Expmentioning

confidence: 99%

“…They are very versatile as formation of the membranes is controlled by the selection of components and conditions used during their assembly. Several studies have shown that stable supported lipid bilayers can be formed on polyelectrolyte multilayers composed of poly(sodium 4-styrenesulfonate) (PSS) and poly(diallyldimethylammonium) chloride (PDADMAC) [37], chitosan (CHI) and hyaluronic acid (HA) [34] or polyethylene imine (PEI) and polystyrene sulfonate (PSS) [38].…”

Section: Introductionmentioning

confidence: 99%