The formation of hyphae of <i>Candida albicans</i> induced by cyclodextrins

Fekete-Forgács, Katalin; Szabó, Erika; Lenkey, Béla

doi:10.1111/j.1439-0507.1997.tb00183.x

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…We obtained similar differences between germ‐tube forming features of the two strains, when the inducer of germination was human serum, anaerobic conditions (data not shown), or N ‐acetyl‐d‐glucosamine (NAGA) ( Table 1). The FR strain proved to be superior in germinating capacity in the presence of cyclodextrins, too [ 25].…”

Section: Resultsmentioning

confidence: 99%

Changes of virulence factors accompanying the phenomenon of induced fluconazole resistance in Candida albicans

Fekete-Forgács¹,

Gyure²,

Lenkey³

2000

Mycoses

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We investigated a fluconazole-sensitive (MICflu = 5 micrograms ml-1) clinical isolate and a fluconazole-resistant (MICflu > 80 micrograms ml-1) laboratory mutant Candida albicans strain developed from the sensitive one. We studied putative virulence factors including germination, adherence ability to either buccal epithelial cells or acrylate surface, the secreted aspartic proteinase, and the extracellular phospholipase activity of the two strains as well as their growth. The fluconazole-resistant strain proved to be superior to the original strain in all the virulence traits tested. The higher virulence of the fluconazole-resistant strain was also supported by a mouse model. These results suggest that the development of fluconazole resistance can be accompanied by serious morphological and physiological changes: several putative virulence traits, moreover the in vivo virulence can increase simultaneously.

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Section: Resultsmentioning

confidence: 99%

Changes of virulence factors accompanying the phenomenon of induced fluconazole resistance in Candida albicans

Fekete-Forgács¹,

Gyure²,

Lenkey³

2000

Mycoses

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“…Complex carbohydrates are known to influence fungal morphology: In S. cerevisiae , starch degradation is associated with the ability to grow invasively and form pseudohyphae (Vivier et al, 1997) and polymeric cyclodextrines were described to induce hyphae by C. albicans (Fekete-Forgács et al, 1997). The repressing effect of glucose on chlamydosporulation, in contrast, was described almost half a century ago for C. albicans (Jansons and Nickerson, 1970b).…”

Section: Resultsmentioning

confidence: 99%

Candida species Rewired Hyphae Developmental Programs for Chlamydospore Formation

Böttcher

Pöllath

Staib³

et al. 2016

Front. Microbiol.

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Chlamydospore formation is a characteristic of many fungal species, among them the closely related human-pathogenic dimorphic yeasts Candida albicans and C. dubliniensis. Whereas function and regulation of filamentation are well-studied in these species, the basis of chlamydospore formation is mostly unknown. Here, we investigate the contribution of environmental and genetic factors and identified central proteins involved in species-specific regulation of chlamydosporulation. We show that specific nutrient levels strongly impact chlamydospore initiation, with starvation favoring sporulation and elevated levels of saccharides or peptone inhibiting it. Thresholds for these nutritional effects differ between C. albicans and C. dubliniensis, which explain species-specific chlamydospore formation on certain diagnostic media. A C. albicans nrg1Δ mutant phenocopied C. dubliniensis, putting Nrg1 regulation at the basis of species-specific chlamydospore formation under various conditions. By screening a series of potential chlamydospore regulators, we identified the TOR and cAMP pathways as crucial for sporulation. As rapamycin treatment blocked chlamydosporulation, a low basal Tor1 activity seems to be essential. In addition, TOR effector pathways play an important role, and loss of the NCR (nitrogen catabolite repression) gene regulators Gat1 and Gln3 reduced chlamydospore formation. A severe reduction was seen for a C. albicans gcn4Δ deletion strain, implicating a link between regulation of amino acid biosynthesis and chlamydospore development. On the other hand, deletion of the GTPase gene RAS1 and the adenylyl cyclase gene CYR1 caused a defect in chlamydospore formation that was mostly rescued by cAMP supplementation. Thus, cAMP-signaling is a second major pathway to control chlamydospore production. Finally, we confirmed light exposure to have a repressive effect on chlamydosporulation. However, permanent illumination only reduced, but not abolished chlamydospore production of C. albicans whereas C. dubliniensis sporulation was unaffected. In summary, we describe novel environmental factors which determine chlamydosporulation and propose a first model for the regulatory network of chlamydospore formation by different Candida species.

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“…Although the inclusion of CDs in the culture medium has been proposed as a useful way to increase growth, cellular development, and the secondary metabolite production of plant tissue cultures, very few reports have analyzed the use of CDs to modulate fungal growth. Fekete‐Forgács et al suggested that CDs were able to induce a yeast‐mycelial transition in Candida albicans and may enter the C. albicans cells as linear oligosaccharides. The encapsulation into CDs of natural antifungal volatile compounds such as hexanal and 2 E ‐hexenal has been proposed as a method to prevent postharvest fungal diseases of food systems .…”

Section: Introductionmentioning

confidence: 99%

Mycelium growth stimulation of the desert truffleTerfezia claveryichatin by β‐cyclodextrin

López‐Nicolás

Pérez‐Gilabert

García-Carmona

et al. 2013

Biotechnology Progress

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The commercial value of Terfezia claveryi, an edible desert truffle with important gastronomic, nutritional, and antioxidant properties, has led to growing interest in its cultivation. The erratic and slow growth of T. claveryi mycelium in vitro represents an impairment to obtain mycorrhizal plants, and it makes necessary to find a new culture medium able to overcome these drawbacks. In this work, we analyze the effect of cyclodextrins (CDs) on the growth of T. claveryi mycelium. Different parameters, including colony diameter, growth rate, and colony fresh weight, were evaluated, both in the presence and absence of these encapsulant agents. The results obtained confirm the ability of CDs to stimulate the growth of T. claveryi mycelium when present in the culture medium. A similar effect was observed when CDs were added to the culture medium of Tuber melanosporum. Three natural (α-, β-, and γ) and two modified (hydroxypropil-β and methyl-β) CDs were assayed. The best results were obtained with β-cyclodextrin, but no improvement was observed with its chemically modified derivatives. CDs complex the different compounds present in the culture medium which impair mycelial growth.

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The formation of hyphae of Candida albicans induced by cyclodextrins

Cited by 5 publications

References 11 publications

Changes of virulence factors accompanying the phenomenon of induced fluconazole resistance in Candida albicans

Changes of virulence factors accompanying the phenomenon of induced fluconazole resistance in Candida albicans

Candida species Rewired Hyphae Developmental Programs for Chlamydospore Formation

Mycelium growth stimulation of the desert truffleTerfezia claveryichatin by β‐cyclodextrin

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