The formation of histotypic fibrous collagen in matrix tissue culture by 3T6 mouse fibroblasts: A response to ascorbic acid

Leighton, Joseph; Estes, Laborary W.; Goldblatt, Peter; Brada, Z

doi:10.1007/bf02617758

Cited by 8 publications

(2 citation statements)

References 10 publications

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“…A density of 25,0000 cells/cm 2 was seeded on the substrates. After 24 h, culture media were replaced with media containing 100 µ M ascorbic acid phosphate (for collagen synthesis [56] , [57] , [58] , [59] ) and 100 µ g/ml carrageenan (for collagen deposition [60] , [61] , [62] , [63] , [64] ). Media were then changed every 3–4 days.…”

Section: Methodsmentioning

confidence: 99%

Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation

Ryan

Pugliese

Shologu

et al. 2023

Biomaterials and Biosystems

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Section: Methodsmentioning

confidence: 99%

Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation

Ryan

Pugliese

Shologu

et al. 2023

Biomaterials and Biosystems

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“…We modified our procedure by using a collagen-coated cellulose sponge to reinforce the fibrin clot (9,10). Furthermore, some types of cells, such as 3T6 fibroblasts, formed three dimensional connective tissue in the interstices of the collagencoated sponge without requiring fibrin clot (11). Other matrices were used sporadically in some studies.…”

Section: Recognizing the Spatial Imperatives Of Epithelial Tissuesmentioning

confidence: 99%

The propagation of cancer, a process of tissue remodeling

Leighton¹,

Tchao²

1984

Cancer Metast Rev

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Effective study of the malignant phenotype at the tissue level requires model systems that are intelligible both to cell biologists and to pathologists, and that also observe the spatial imperatives intrinsic to tissues in nature. Malignant cells commonly appear in multicellular units, and growth of tumor tissue is seen as an increase in the number of cells and multicellular units. The supporting stroma frequently has an abnormal appearance, and this component of the tissue also increases in mass as the tumor enlarges and spreads. The direction of invasion is influenced by the direction of available metabolites. Histophysiologic gradient culture complies with nature's spatial rationale, since at the substrate-parenchymal interface three functions coincide. These are anchorage, initiation of epithelial renewal, and complete exchange of metabolites. Our model system provides a setting for reconstructing and manipulating many features of the malignant phenotype seen in cancer tissues in nature, such as abnormalities in the sequence of maturation of stratified epithelium, hyperplasias, dysplasias, interaction between different types of epithelium, aggregate formation, tumor angiogenesis, and neoplastic blockade.

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Phenotypic diversification of a cultured tumor line as a function of substratum

Dugan

Macario

1981

J. Supramol. Struct. Cell. Biochem.

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We have found that a murine hepatoma displays a considerable phenotypic diversification in culture, which depends upon the substratum utilized, and is manifested by the formation of multicellular structures of differing geometry: Monolayer on glass and plastic, thick multilayer pads on Gelfilm, and spheroids on agar and agarose. These multicellular morphological phenotypes were assayed without disruption to ascertain their antigenicity in vitro and their tumorigenicity in vivo and to obtain quantitative information on the effect of the spatial arrangement of the hepatoma cells upon the ability of each multicellular structure to interact, as a whole, with molecules and cells in its surroundings. The antigenicity of the multicellular structures was determined with calibrated probes and a methodology that measures the total antigenicity, as well as antigenicity per unit of surface area. Antigenicity was found to differ in the following decreasing order: Monolayer on plastic greater than spheroids on agarose greater than spheroids on agar greater than multilayer on Gelfilm. At least part of these antigenic variants arise from different degrees of masking of the structures' surface determinants by a trypsin-sensitive material. The multicellular phenotypes also differed in tumorigenicity. When assayed in syngeneic hosts under comparable conditions, agar-grown spheroids produced the fewest tumors, whereas Gelfilm-grown multilayers produced the most. These two independent sets of data show that the various geometries that a tumor tissue is induced to acquire by the culture substratum are accompanied by a distinctive combination of surface and biological properties.

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The formation of histotypic fibrous collagen in matrix tissue culture by 3T6 mouse fibroblasts: A response to ascorbic acid

Cited by 8 publications

References 10 publications

Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation

Physicochemical cues are not potent regulators of human dermal fibroblast trans-differentiation

The propagation of cancer, a process of tissue remodeling

Phenotypic diversification of a cultured tumor line as a function of substratum

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