The formation of germ tubes byCandida albicansin various peptone media

Evaluation of Different Media for Germ Tube Production of Candida Albicans and Candida Dubliniensis

“…On the other hand, Arora DR et al 7 7 reported 61.8% positivity in bactopeptone which is in agreement with our study. In contrast to our observation, Joshi KR et al 15 reported, 100% of C. albicans to be positive for germ tube test in 1% bactopeptone whereas only 74% were positive in trypticase soy broth.…”

Whey-derived free fatty acids suppress the germination ofCandida albicans in vitro

“…Each of these three fractions was assayed for its ability to inhibit germination of C. albicans in vitro . Germination was induced at 37°C using dextrose‐free Sabouraud medium as described previously (Joshi et al , 1973). Because inhibition of germination by these molecules could be due to an early event (i.e.…”

“…These late exponentially growing cells were then washed twice with water and kept on ice until used. Germination was induced by incubating cells (final density of 5 × 10 5 cells mL −1 ) at 37°C in 2.5 mL of either dextrose‐free Sabouraud medium (Joshi et al , 1973), Lee's medium (Lee et al , 1975), Spider medium (Liu et al , 1994) or hypha‐forming medium (Biswas et al , 2001), supplemented with free fatty acids. All lipids were dissolved in ethanol.…”

Germ tube formation from zonal rotor fractions of Candida albicans

“…Candida albicans is a dimorphic fungus which can undergo transition from yeastlike growth to mycelial growth. This transition takes place in a variety of media: serum (11), complex medium (5), minimal medium (6,7), and N-acetyl-D-glucosamine in imadazole-Mn2+ buffer (9). The synchrony or asynchrony with which cultures undergo this transition may depend both on the state of the cell at the beginning of induction of the yeast-mycelial transition and the homogeneity of the population.…”