The forgotten sugar: A review on multifarious applications of melezitose

Balaji, S.

doi:10.1016/j.carres.2021.108248

Cited by 12 publications

(11 citation statements)

References 81 publications

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“…The presence of fatty acids like 17‐octadecynoic acid (23.85 min), oleic acid (25.64 min), and phytol make MP leaves more nutritional or nutraceutical favorable. Melezitose is considered as a masking agent in the food industry and as a replacement of sugar additives [17] . It is the molecule that suppresses the unpleasant taste or sensation, i. e., organoleptic parameters [17] .…”

Section: Resultsmentioning

confidence: 99%

“…Melezitose is considered as a masking agent in the food industry and as a replacement of sugar additives [17] . It is the molecule that suppresses the unpleasant taste or sensation, i. e., organoleptic parameters [17] . The presence of fatty acids acids in MP makes it more medicinally potent as these phytochemicals have anti‐inflammatory, anticancer and anti‐oxidant properties [18] .…”

Section: Resultsmentioning

confidence: 99%

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Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under‐Utilized Part

Gandhi,

Prasad,

Kumar

et al. 2023

Chemistry & Biodiversity

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Mimosa pudica L. (MP) is well‐known plant in traditional medicinal system, especially in India. Unfortunately, leaves of MP are less explored. To determine the food and nutritional value of the neglected part of Mimosa pudica L. (MP), that is MP leaves, phytochemicals and metal ions of MP were quantified by newly developed HPLC and ICPOES‐based methods. The content of phytochemicals observed using HPLC analysis for chlorogenic acid, catechin, and epicatechin was 141.823 (±8.171), 666.621 (±11.432), and 293.175 (±12.743) μg/g, respectively. Using GC/MS/MS analysis, fatty acid like oleic acid were identified. In ICP‐OES analysis, a significant content of Na, K, Ca, Cu, Fe, Mg, Mn, and Zn was observed. The observed TPC and TFC for MP leaf extracts was 44.327 (±1.041) mg GAE/ g of wt. and 214.217 (±4.372) mg QCE/ g of wt., respectively. The DPPH assay depicted a strong antioxidant activity of MP leaf extracts with IC50 values of 0.796 (±0.081) mg/mL and a TEAC value of 0.0356 (±0.0003). A significant antacid activity (666 mg MP+400 mg CaCO3 >400 mg CaCO3 ≫666 mg Gelusil) of MP leaves was noticed. The methanolic extract of MP leaves demonstrated anti‐microbial activity against Staphylococcus aureus (15±2mm), Pseudomonas aeruginosa (12±2mm) and Escherichia coli (10±2mm). In silico studies confirmed the in vitro results obtained for antioxidant, antiacid, and anti‐microbial activities. In addition, in silico studies revealed the anti‐cancerous and anti‐inflammatory potential of the MP leaves. In summary, this study demonstrated the medicinal significance of MP leaves and the conversion of agro‐waste or the under‐utilized part of MP into pharmaceutical potent materials. Consequently, the present study highlighted that MP leaves alone have medicinal importance with good nutritional utility and possess large promise in the pharma industry along with improving bio‐valorization and the environment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under‐Utilized Part

Gandhi,

Prasad,

Kumar

et al. 2023

Chemistry & Biodiversity

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“…To answer this question, we turned to a 10-gene pathway (15.4 kb) taken from Bifidobacterium breve UC2003 that enables E. coli to consume the trisaccharide melezitose. Melezitose is a natural sugar that is also a prebiotic candidate ( 59 , 60 ). Wildtype E. coli C600 grows slowly in melezitose minimal media, taking more than 6 hours to reach OD 600 0.2 and 24 hours to reach OD 0.43.…”

Section: Resultsmentioning

confidence: 99%

Inducible directed evolution of complex phenotypes in bacteria

Al'Abri

Haller

et al. 2022

Nucleic Acids Research

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Directed evolution is a powerful method for engineering biology in the absence of detailed sequence-function relationships. To enable directed evolution of complex phenotypes encoded by multigene pathways, we require large library sizes for DNA sequences >5–10 kb in length, elimination of genomic hitchhiker mutations, and decoupling of diversification and screening steps. To meet these challenges, we developed Inducible Directed Evolution (IDE), which uses a temperate bacteriophage to package large plasmids and transfer them to naive cells after intracellular mutagenesis. To demonstrate IDE, we evolved a 5-gene pathway from Bacillus licheniformis that accelerates tagatose catabolism in Escherichia coli, resulting in clones with 65% shorter lag times during growth on tagatose after only two rounds of evolution. Next, we evolved a 15.4 kb, 10-gene pathway from Bifidobacterium breve UC2003 that aids E. coli’s utilization of melezitose. After three rounds of IDE, we isolated evolved pathways that both reduced lag time by more than 2-fold and enabled 150% higher final optical density. Taken together, this work enhances the capacity and utility of a whole pathway directed evolution approach in E. coli.

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“…A study reported a low acid hydrolysis and low α-glucosidase hydrolysis susceptibility, which may fulfil the prebiotic criterion of human indigestibility for melezitose. 30 This compound has promising pharmaceutical applications. It is used as an excipient to optimize pharmaceutical formulations and to stabilize proteins during recombinant protein therapy by protein glycosylation.…”

Section: Discussionmentioning

confidence: 99%

Acacia Honey-derived Bioactive Compounds Exhibit Induction of p53-dependent Apoptosis in the MCF-7 Human Breast Cancer Cell Line

Hamadou

Bouali

Alhejaili

et al. 2023

Pharmacognosy Magazine

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Background Research studies have focused on discovering new anti-proliferative and pro-apoptotic agents derived from natural products from which honey constitutes a prominent candidate. The Acacia honey (AH) is known to display anticancer activity, but the mechanisms of action are still not well defined. Objectives Using in vitro and computational approaches, we aimed to assess the interaction among selected bioactive compounds derived from AH, with the apoptotic protein p53, which could trigger apoptosis. Methods The phytocompounds of AH were investigated via gas chromatography–mass spectrophotometry analysis. The cytotoxic effect and induced apoptosis on the MCF-7 breast cancer cell line were assessed by 3-(4,5-dimethylthiazolyl-2)-2,5 diphenyltetrazolium bromide and acridine orange-ethidium bromide staining approaches. The molecular docking analysis between AH compounds and p53 was carried out. Results The drug-likeness prediction revealed that most of the identified compounds meet Lipinski’s rules. We demonstrate that AH exerts an interesting cytotoxic effect in a dose-dependent manner against the MCF-7 cell line with IC50 5.053µg/mL. Significant cell alterations and notable induced apoptosis were detected when cells were treated with AH. The molecular docking analysis revealed that melezitose is among the most important potential bioactive compounds that interact with p53 leading to apoptosis. The binding affinity was −8.1 kcal/mol, and the closest molecular interactions in the active site included 10 residues, which could explain the potential biological activity. Conclusion This work sheds light on AH as a significant source of bioactive chemicals with potential for promoting apoptosis that may be exploited as an alternative therapy for breast cancer.

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The forgotten sugar: A review on multifarious applications of melezitose

Cited by 12 publications

References 81 publications

Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under‐Utilized Part

Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under‐Utilized Part

Inducible directed evolution of complex phenotypes in bacteria

Acacia Honey-derived Bioactive Compounds Exhibit Induction of p53-dependent Apoptosis in the MCF-7 Human Breast Cancer Cell Line

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