The Footprints of Oxidative Stress and Mitochondrial Impairment in Arsenic Trioxide-Induced Testosterone Release Suppression in Pubertal and Mature F1-Male Balb/c Mice via the Downregulation of 3β-HSD, 17β-HSD, and CYP11a Expression

Ommati, Mohammad Mehdi; Heidari, Reza; Zamiri, Mohammad Javad; Sabouri, Samira; Zaker, Ladan; Farshad, Omid; Jamshidzadeh, Akram; Mousapour, Saeed

doi:10.1007/s12011-019-01815-2

Cited by 28 publications

(16 citation statements)

References 52 publications

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“…Sperm motility, as a potential index of fertilizing ability, can adversely be affected by environmental (94,95), industrial (96,97), and pharmaceutical agents (98). In line with previous researches (20,21,(26)(27)(28)(29)(30)(31)57), we also observed a significant decrement in sperm viability, forward motility, plasma membrane integrity (HOS test), and sperm count, as well as an increment in sperm abnormality following treatment with CNTs. Severe OS along with mitochondrial dysfunction would impair the spermatogenesis and fertility through decrements in sperm viability, count, motility, and steroidogenesis, and an increment in the percentage of abnormal sperm, as well as alterations in histomorphology of the testes or accessory sex organs (20,(26)(27)(28)30).…”

Section: Discussionsupporting

confidence: 90%

“…Mitochondrial impairment due to OS induction by CNTs in the present experiment could be an essential factor in CNTinduced reproductive toxicity. There are ample data in the literature showing that the abnormal levels of cellular glutathione (oxidized/reduced GSH) are closely interconnected with the induction of OS in reproductive organs (20,21,27,29); therefore, CNT-induced decreases in reduced GSH levels, and increases in oxidized GSH level in male gonads and gametes could trigger severe OS and complications. Spermatozoa are very sensitive to peroxidation due to the high level of polyunsaturated fatty acids (PUFAs) in the plasma membrane (26,32), adversely affecting the fertility potential (26,33,34).…”

Section: Discussionmentioning

confidence: 99%

“…Buffer A (water: methanol; 1:4 v: v) and buffer B (acetate buffer: methanol; 1:4 v: v) were used as the mobile phases. A gradient method with a steady increase of buffer B to 95% in 20 min was used (29,62), where the nanomolar levels of GSH and GSSG can be determined (62); GSSG and GSH were used as external standards. Specimens of testicular tissue (200 mg) and spermatozoa (10 6 /mL) were homogenized in 250 mM Tris-HCl buffer (pH = 7.4) at 4 • C. Afterward, 500 µL of TCA (50% w:v, 4 • C) were added to each homogenized sample.…”

Section: Testicular and Sperm Glutathione Contentmentioning

confidence: 99%

“…Oxidative stress (OS), mitochondrial impairment, and their subsequent complications seem to play crucial roles in the xenobiotics-induced reprotoxicity and consequent infertility (20,21,(24)(25)(26)(27)(28)(29)(30). High sensitivity to OS, excessive reactive oxygen species (ROS) levels, and thiobarbituric acid reactive substances (TBARS) content could dramatically affect the functions of the reproductive organs (20,21,(26)(27)(28)(29)(30)(31)(32)(33)(34). For instance, oxidative injury and DNA damage studies indicated that high doses of MWCNT could cause DNA damage in murine sperm (35).…”

Section: Introductionmentioning

confidence: 99%

“…Cellular energy metabolism is pivotal in sperm forward motility (24,25,51), and there is ample evidence showing that xenobiotics could alter mitochondrial functionality and induce severe OS in different reproductive tissues (20,21,(27)(28)(29)(30). In this regard, Xu et al (52) reported that MWCNT caused severe damages to mitochondrial DNA in spermatocytes.…”

Section: Introductionmentioning

confidence: 99%

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Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Carbon-based nanomaterials possess a remarkably high potential for biomedical applications due to their physical properties; however, their detrimental effects on reproduction are also concerned. Several reports indicate the toxicity of carbon nanotubes (CNT); nevertheless, their impact on intracellular organelles in the male reproductive organs has not been fully elucidated. Herein, we report on the reprotoxicity of single-walled (SWCNT) and multi-walled carbon nanotubes (MWCN) on several intracellular events and histological criteria in pubertal male BALB/c mice orally treated with 0, 10, and 50 mg/kg/day doses for 5 weeks. Biomarkers of oxidative stress and mitochondrial functionality, histopathological alterations, and epididymal sperm characteristics were determined. Oral administration of CNTs at 10 and 50 mg/kg evoked a significant decrement in weight coefficient, sperm viability and motility, hypo-osmotic swelling (HOS) test, sperm count, mitochondrial dehydrogenase activity, ATP content, total antioxidant capacity, and GSH/GSSH ratio in the testis and epididymal spermatozoa. On the other hand, percent abnormal sperm, testicular and sperm TBARS contents, protein carbonylation, ROS formation, oxidized glutathione level, and sperm mitochondrial depolarization were considerably increased. Significant histopathological and stereological alterations in the testis occurred in the groups challenged with CNTs. The current findings indicated that oxidative stress and mitochondrial impairment might substantially impact CNTs-induced reproductive system injury and sperm toxicity. The results can also be used to establish environmental standards for CNT consumption by mammals, produce new chemicals for controlling the rodent populations, and develop therapeutic approaches against CNTs-associated reproductive anomalies in the males exposed daily to these nanoparticles.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Testicular and Sperm Glutathione Contentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

et al. 2020

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Glycine protects the male reproductive system against lead toxicity via alleviating oxidative stress, preventing sperm mitochondrial impairment, improving kinematics of sperm, and blunting the downregulation of enzymes involved in the steroidogenesis

Ommati

Ahmadi

Sabouri

et al. 2022

Environmental Toxicology

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Lead (Pb) is a highly toxic heavy metal widely dispersed in the environment because of human industrial activities. Many studies revealed that Pb could adversely affect several organs, including the male reproductive system. Pb‐induced reproductive toxicity could lead to infertility. Thus, finding safe and clinically applicable protective agents against this complication is important. It has been found that oxidative stress plays a fundamental role in the pathogenesis of Pb‐induced reprotoxicity. Glycine is the simplest amino acid with a wide range of pharmacological activities. It has been found that glycine could attenuate oxidative stress and mitochondrial impairment in various experimental models. The current study was designed to evaluate the role of glycine in Pb‐induced reproductive toxicity in male mice. Male BALB/c mice received Pb (20 mg/kg/day; gavage; 35 consecutive days) and treated with glycine (250 and 500 mg/kg/day; gavage; 35 consecutive days). Then, reproductive system weight indices, biomarkers of oxidative stress in the testis and isolated sperm, sperm kinetic, sperm mitochondrial indices, and testis histopathological alterations were monitored. A significant change in testis, epididymis, and Vas deferens weight was evident in Pb‐treated animals. Markers of oxidative stress were also significantly increased in the testis and isolated sperm of the Pb‐treated group. A significant disruption in sperm kinetic was also evident when mice received Pb. Moreover, Pb exposure caused significant deterioration in sperm mitochondrial indices. Tubular injury, tubular desquamation, and decreased spermatogenic index were histopathological alterations detected in Pb‐treated mice. It was found that glycine significantly blunted oxidative stress markers in testis and sperm, improved sperm mitochondrial parameters, causing considerable higher velocity‐related indices (VSL, VCL, and VAP) and percentages of progressively motile sperm, and decreased testis histopathological changes in Pb‐exposed animals. These data suggest glycine as a potential protective agent against Pb‐induced reproductive toxicity. The effects of glycine on oxidative stress markers and mitochondrial function play a key role in its protective mechanism.

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The crucial role of oxidative stress in non-alcoholic fatty liver disease-induced male reproductive toxicity: the ameliorative effects of Iranian indigenous probiotics

Ommati

Jamshidzadeh

et al. 2022

Naunyn-Schmiedeberg's Arch Pharmacol

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The Footprints of Oxidative Stress and Mitochondrial Impairment in Arsenic Trioxide-Induced Testosterone Release Suppression in Pubertal and Mature F1-Male Balb/c Mice via the Downregulation of 3β-HSD, 17β-HSD, and CYP11a Expression

Cited by 28 publications

References 52 publications

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Spermatotoxic Effects of Single-Walled and Multi-Walled Carbon Nanotubes on Male Mice

Glycine protects the male reproductive system against lead toxicity via alleviating oxidative stress, preventing sperm mitochondrial impairment, improving kinematics of sperm, and blunting the downregulation of enzymes involved in the steroidogenesis

The crucial role of oxidative stress in non-alcoholic fatty liver disease-induced male reproductive toxicity: the ameliorative effects of Iranian indigenous probiotics

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