The food and pharmaceutical additive benzoic acid induces amyloid fibrillation of an intrinsically disordered protein

Ramesh, Halavath; Bhuyan, Abani K.

doi:10.1016/j.bpc.2024.107172

Cited by 1 publication

(1 citation statement)

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“…While studying aspects of food preservative binding to globular proteins, we found in 2021 that the benzoate class of food additives perturbs the native states of lysozyme, cytochrome c, and myoglobin and transforms the protein population to a mixture of amorphous and amyloid fibrillar aggregates, presumably via denatured or quasi-native states . A study soon after showed that benzoates induce amyloid fibrillation of an intrinsically disordered protein (IDP) as well, raising concerns about the safety and use of synthetic food additives. To explore the effect of food additives on protein structure and conformation further, we sought to study the influence of synthetic food colorants that unlike natural colors are expected to substantially perturb protein structures.…”

Section: Introductionmentioning

confidence: 99%

Food Colorants Acid Yellow 23, Azorubine, and Acid Red 18 Cause Aggregation and Fibrillation of Proteins

Ramesh,

Bhuyan

2024

ACS Food Sci. Technol.

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Acid Yellow 23 (AY23), Azorubine (AR), and Acid Red 18 (AR18) are three of a string of approved synthetic food colorants, each of whose maximal daily intake is set to about 100 mg kg–1 day–1. This study deploys spectroscopy, electron microscopy, and X-ray diffraction (XRD) to examine the effect of dye binding to lysozyme, cytochrome c, myoglobin, and bovine serum albumin (BSA) under acidic and neutral pH conditions. The c 1/2 value for binding of food dyes to globular proteins in vitro is lowest for AR18 (∼50 μM) but increases with AY23 (∼140 μM) and AR (∼190 μM). Dye binding reversibly transforms native proteins to quasi-native states, which enter the aggregation pathway to yield an initial amorphous mesopaque that reconfigures at longer times to produce crystalline fibrillar forms. In vivo protein aggregation is, however, unlikely because of a substantial negative gradient from intestinal to systemic concentrations of the dyes.

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Section: Introductionmentioning

confidence: 99%