2014
DOI: 10.3791/50442
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The FlyBar: Administering Alcohol to Flies

Abstract: Fruit flies (Drosophila melanogaster) are an established model for both alcohol research and circadian biology. Recently, we showed that the circadian clock modulates alcohol sensitivity, but not the formation of tolerance. Here, we describe our protocol in detail. Alcohol is administered to the flies using the FlyBar. In this setup, saturated alcohol vapor is mixed with humidified air in set proportions, and administered to the flies in four tubes simultaneously. Flies are reared under standardized conditions… Show more

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Cited by 12 publications
(18 citation statements)
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“…To better understand how the circadian regulation of alcohol sensitivity changes with age, we examined a second alcohol behavior, alcohol-induced sedation, across age groups. Sedation is the induction of a sleep state characterized by immobility (De Nobrega and Lyons, 2016, van der Linde et al, 2014). We observed a robust rhythm in alcohol-induced sedation in young (3 d old) flies (Fig.…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…To better understand how the circadian regulation of alcohol sensitivity changes with age, we examined a second alcohol behavior, alcohol-induced sedation, across age groups. Sedation is the induction of a sleep state characterized by immobility (De Nobrega and Lyons, 2016, van der Linde et al, 2014). We observed a robust rhythm in alcohol-induced sedation in young (3 d old) flies (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Alcohol delivery occurred using an alcohol vapor system in the dark as previously described (De Nobrega and Lyons, 2016, van der Linde et al, 2014, van der Linde and Lyons, 2011). Briefly, four tubes of flies (approximately 30 flies per tube) were exposed to a mixture of alcohol vapor and humidified air at a predetermined ratio of 95% alcohol and de-ionized water (Koptec, Declon Labs, Inc. King of Prussia, PA).…”
Section: Methodsmentioning
confidence: 99%
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“…Exposure to alcohol vapor was performed as described previously (van der Linde and Lyons, 2011; van der Linde et al, 2014). Four tubes of Drosophila (3–5 days old; ~30 flies per tube) were exposed to ethanol vapor by mixing proportional fractions of an airstream bubbled through deionized water with an airstream bubbled through 95% ethanol (Koptec) to achieve the pre-determined percentage of ethanol vapor.…”
Section: Methodsmentioning
confidence: 99%
“…The loss-of-righting reflex assay was performed as previously described in Drosophila (van der Linde and Lyons, 2011; van der Linde et al, 2014). Prior to the start of alcohol exposure, flies were habituated to dark room conditions for one hour (25° Celsius, 60 – 70 % humidity).…”
Section: Methodsmentioning
confidence: 99%