The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical Properties

Meyer, Jean Marie; Abdallah, Mohamed Abou-Elwafa

doi:10.1099/00221287-107-2-319

Cited by 739 publications

(655 citation statements)

References 18 publications

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“…As seen in Figure 1, the fluorescence of P. aeruginosa is diffusive with a peak located at 470 nm. This is in agreement with the position of the pyoverdin fluorescence maximum given in the literature [18,22]. Likewise the excitation fluorescence spectrum with a maximum close to 400 nm is identical to the absorption and excitation spectra of pyoverdin reported in the literature [18,21].…”

Section: Resultssupporting

confidence: 80%

Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

Усачева¹,

Teichert²,

Usachev

et al. 2008

Lasers Surg Med

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Background and Objectives: The difference in photobactericidal efficacy between methylene blue (MB) and toluidine blue (TB) may be explained by their involvement with proteins, lipopolysaccharides (LPS), and siderophores and siderophore-receptor protein complexes on the bacterial outer membrane. This study aims to determine if this is the case by using the fluorescence given off by a pseudomonal siderophore named pyoverdin. Study Design/Materials and Methods: Confocal laser scanning microscopy was used to observe the fluorescence of Pseudomonas aeruginosa cells excited at 488 nm in the presence of increasing dye concentrations. Results: Cellular fluorescence at 522 nm progressively decreased with increasing dye concentrations. The SternVolmer constants for cellular fluorescence quenching with the dyes were compared to the association constants for dyes complexed with LPS. The quenching of cellular fluorescence was associated with the formation of a ground-state complex between the dyes and pyoverdin-FpvA protein system. MB readily complexed with this system, whereas TB complexed more strongly with LPS. Conclusion: The different affinities of the dyes for both pyoverdin-protein and LPS will affect the contributions of the dyes' interactions with these biopolymers to the overall bacterial photodamage.

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Section: Resultssupporting

confidence: 80%

Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

Усачева¹,

Teichert²,

Usachev

et al. 2008

Lasers Surg Med

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“…Pyoverdine was firstly discovered in 1892 and its function as iron chelator and transporter was determined in the late 1970s by Meyer and Abdallah (1978). Chemical structure of pyoverdine is generally consisted of three parts: one is the conserved fluorescent dihydroxyquinoline chromophore; another is the acyl side chain bounded to the amino group of chromophore; and the third part is a variable peptide chain bound to the carboxyl group of chromophore (Visca et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Yin

Zhang

Chen

2014

Biosensors and Bioelectronics

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a b s t r a c tMethods for the rapid and sensitive detection of furazolidone, a pesticide used for the treatment of infections of animals and human beings, have been urgently recommended for its large residual, strong carcinogenicity and genotoxicity in the environment. In this study, a method for the detection of furazolidone based on the rapid fluorescence quenching of pyoverdine by furazolidone was developed. Pyoverdine secreted by a Pseudomonas aeruginosa strain PA1 was purified through affinity chromatography and its fluorescent property was characterized. The fluorescence of pyoverdine could be quenched by furazolidone with specificity, and based on this phenomenon a fluorescent method for furazolidone detection was established. Fluorescence of pyoverdine was quenched by furazolidone probably due to the electron transfer from pyoverdine to furazolidone. The optimal pH for the detection was 7.2 in 50 mM 3-(N-Morpholino) propanesulfonic acid solution, and the whole detection process could be completed within a few seconds. The linear range of the detection was 2-160 mM and the limit of detection (LOD) was 0.5 mM. This study developed a novel fluorescent method for furazolidone detection, and the rapid and specific fluorescent biosensor can be potentially applied for furazolidone detection in the aquatic samples.

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“…nutritional iron deficiency. This is inferred by the evidence that pyoverdine synthesis is repressed in suspension cultures grown in chemically-defined laboratory media containing more than 5 μM FeCl3 (Meyer and Abdallah 1978). Iron-dependent transcriptional repression is primarily dependent on the binding of the Fur-Fe (II) holorepressor complex to the promoters of iron-regulated genes.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Detection of Genes Responsible for Pyoverdines Biosynthesis in Pseudomonas putida KNUK9

Hussein¹,

Joo²

2015

Korean Journal of Soil Science and Fertilizer

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Pyoverdines (PVDs) are organic compounds produced by the fluorescent Pseudomonads under iron starvation conditions. Among the isolated rhizosphere pseudomonads strains, P. putida KNUK9 showed the highest production of PVDs and its production reached to 62.81% siderophores units. DNA isolation, ligation, PCR amplification, and transformation using E. coli DH5α cells were carried out for preparing the strong pyoverdine producer strains. We detected seven genes playing the fundamental roles in the pyoverdine metabolism in Pseudomonads. According to data and analysis obtained from the study, we deduced that the strain P. putida KNUK9 contains the essential genes required for pyoverdine biosynthesis. Key words: Pseudomonas putida, Pyoverdines genes, Biosynthesis, DetectionAgarose gel image of the pyoverdine genes pA; PvdA, pD; PvdD, pH; PvdH, pL; PvdL solated from P. putida KNUK9.

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The Fluorescent Pigment of Pseudomonas fluorescens: Biosynthesis, Purification and Physicochemical Properties

Cited by 739 publications

References 18 publications

Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

Interaction of the photobactericides methylene blue and toluidine blue with a fluorophore in Pseudomonas aeruginosa cells

Pyoverdine secreted by Pseudomonas aeruginosa as a biological recognition element for the fluorescent detection of furazolidone

Isolation and Detection of Genes Responsible for Pyoverdines Biosynthesis in Pseudomonas putida KNUK9

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