The fluorescence of dye-nucleic acid complexes

Löber, G.

doi:10.1016/0022-2313(81)90022-3

Cited by 67 publications

(21 citation statements)

References 232 publications

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“…In view of the fact that the appreciable displacement of the absorption spectrum of the dye to longer wavelengths is observed (about 10 nm) at high DNA concentration, it is believed that a dye molecule intercalates between two base pairs of a DNA molecule to which it is bound [3].…”

Section: Resultsmentioning

confidence: 99%

“…These complexes are in the first place investigated in vitro. Nevertheless, there is good evidence that they are also present in biological systems where the nucleic acids exist in their natural states [3].…”

Section: Introductionmentioning

confidence: 99%

“…The DNA double strand defines special interaction of a number of organic molecules and leads to complex formation with high binding constants (10 4 -10 7 M −1 ) [3]. Acridine orange, methylene blue, Bengal rose, ethydium bromide, thiazole orange are dyes, which have wide application in biochemistry and photobiology due to their capacity to form intermolecular complexes with deoxyribonucleic acid (DNA) under free energy changes of less than 10 kcal/mol (42 kJ/mol).…”

Section: Introductionmentioning

confidence: 99%

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Complex formation between 3;3´‐diethylthiacarbocyanine iodide and DNA and its investigation in aqueous solution

Anikovsky

Татиколов

Кузьмин

1999

International Journal of Photoenergy

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Abstract. The binding of 3, 3 -diethylthiacarbocyanine iodide (DTCC) with molecules of DNA was investigated by different photochemical methods. Absorption spectra were investigated under various concentration of DNA. Isosbestic points observed in these spectra are attributable to existence of two different complexes DTCC with DNA. Equilibrium constants of the formation of complexes I and II were determined (K 1 ≈ 10 6 M −1 , K 2 ≈ 5 × 10 4 M −1 accordingly). Differential absorption spectra of two photoisomers of DTCC were obtained by flash photolysis method; it was found that the quantum yield of the short-lived photoisomer decreases and the quantum yield of the second long-lived photoisomer builds up with the increase in the DNA concentration. Kinetics of interaction between the triplet state of the dye and oxygen were investigated under various concentration of DNA. The growth of the quantum yield of fluorescence and intersystem crossing was observed with the increase in the DNA concentration.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Complex formation between 3;3´‐diethylthiacarbocyanine iodide and DNA and its investigation in aqueous solution

Anikovsky

Татиколов

Кузьмин

1999

International Journal of Photoenergy

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“…Alguns artigos [188][189][190][191][192] explicam a redução da intensidade de fluorescência da AL e derivados da mesma na ligação com os sítios de DNA pela ligação do corante com os pares de guanina-citosina (G-C). Nos sítios G-C a fluorescência do corante é suprimido por sua interação com a guanina.…”

Section: Interação Da Acridina Laranja Com Dna: Efeito Da Força Iônicaunclassified

“…O caráter bi-exponencial da cinética de decaimento da fluorescência da AL pode estar associado com a ligação da molécula de AL ligando-se a dois tipos distintos de sítios de DNA, tal como os pares G-C e A-T, por exemplo [166,[188][189][190][191][192]. Contudo, nesse caso haveria o aumento simultâneo da concentração de ambos os sítios G-C e A-T com o aumento da concentração de DNA.…”

Section: Cinéticas Do Decaimento Da Fluorescênciaunclassified

Estudos da agregação de corantes ciânicos em soluções aquosas homogêneas e na presença de nanoestruturas

Amado¹

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Hybridization‐Sensitive On–Off DNA Probe: Application of the Exciton Coupling Effect to Effective Fluorescence Quenching

Ikeda

Okamoto

2008

Chemistry An Asian Journal

107

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The design of dyes that emit fluorescence only when they recognize the target molecule, that is, chemistry for the effective quenching of free dyes, must play a significant role in the development of the next generation of functional fluorescent dyes. On the basis of this concept, we designed a doubly fluorescence-labeled nucleoside. Two thiazole orange dyes were covalently linked to a single nucleotide in a DNA probe. An absorption band at approximately 480 nm appeared strongly when the probe was in a single-stranded state, whereas an absorption band at approximately 510 nm became predominant when the probe was hybridized with the complementary strand. The shift in the absorption bands shows the existence of an excitonic interaction caused by the formation of an H aggregate between dyes, and as a result, emission from the probe before hybridization was suppressed. Dissociation of aggregates by hybridization with the complementary strand resulted in the disruption of the excitonic interaction and strong emission from the hybrid. This clear change in fluorescence intensity that is dependent on hybridization is useful for visible gene analysis.

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The fluorescence of dye-nucleic acid complexes

Cited by 67 publications

References 232 publications

Complex formation between 3;3´‐diethylthiacarbocyanine iodide and DNA and its investigation in aqueous solution

Complex formation between 3;3´‐diethylthiacarbocyanine iodide and DNA and its investigation in aqueous solution

Estudos da agregação de corantes ciânicos em soluções aquosas homogêneas e na presença de nanoestruturas

Hybridization‐Sensitive On–Off DNA Probe: Application of the Exciton Coupling Effect to Effective Fluorescence Quenching

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