The Fluidity of the Bacterial Outer Membrane Is Species Specific

Cao, Pengbo; Wall, Daniel

doi:10.1002/bies.201900246

Cited by 13 publications

(6 citation statements)

References 72 publications

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“…The low fluidity of the OMV coating is in line with the common imagination of the bacterial OM to be highly rigid. [ 56 ] However, considering the fluidity of OMV‐derived lipid bilayers from either E. coli [ 57 ] or other Gram‐negative species, [ 55 ] the fluidity of this coating appears particularly low, which gives the impression that the functionality of lipids has been dramatically decreased. This is explainable by the additional presence of LPS and proteins among the OMV PLs.…”

Section: Resultsmentioning

confidence: 99%

An Outer Membrane Vesicle‐Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability

Richter

Kamal

Koch

et al. 2021

Adv Healthcare Materials

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When searching for new antibiotics against Gram-negative bacterial infections, a better understanding of the permeability across the cell envelope and tools to discriminate high from low bacterial bioavailability compounds are urgently needed. Inspired by the phospholipid vesicle-based permeation assay (PVPA), which is designed to predict non-facilitated permeation across phospholipid membranes, outer membrane vesicles (OMVs) of Escherichia coli either enriched or deficient of porins are employed to coat filter supports for predicting drug uptake across the complex cell envelope. OMVs and the obtained in vitro model are structurally and functionally characterized using cryo-TEM, SEM, CLSM, SAXS, and light scattering techniques. In vitro permeability, obtained from the membrane model for a set of nine antibiotics, correlates with reported in bacterio accumulation data and allows to discriminate high from low accumulating antibiotics. In contrast, the correlation of the same data set generated by liposome-based comparator membranes is poor. This better correlation of the OMV-derived membranes points to the importance of hydrophilic membrane components, such as lipopolysaccharides and porins, since those features are lacking in liposomal comparator membranes. This approach can offer in the future a high throughput screening tool with high predictive capacity or can help to identify compound-and bacteria-specific passive uptake pathways.

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Section: Resultsmentioning

confidence: 99%

An Outer Membrane Vesicle‐Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability

Richter

Kamal

Koch

et al. 2021

Adv Healthcare Materials

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“…This model can accommodate a number of scenarios for how JspA and LppA jointly respond to envelope stress: for instance, LppA may enable the proper folding or positioning of JspA in the membrane, or misfolding of LppA may directly induce JspA's proteolytic activity. In particular, the complex physical properties of the outer membrane (Cao & Wall, 2020;Sun et al, 2022) necessitate multiple regulatory checkpoints, and LppA may sense its integrity, analogous to the E. coli RcsF lipoprotein (Tata et al, 2021), and transmit disturbances to JspA. Alternatively, LppA may monitor or participate in the crosslink between the outer membrane and the cell wall (Godessart et al, 2021), and disruptions in the process are relayed to JspA.…”

Section: Discussionmentioning

confidence: 99%

A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical inSinorhizobium melilotifor symbiosis with legume hosts

Bustamante,

Ceron,

Gao

et al. 2023

Preprint

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Sinorhizobium melilotiis a model alpha-proteobacterium for investigating microbe-host interactions, in particular nitrogen-fixing rhizobium-legume symbioses. Successful infection requires complex coordination between compatible host and endosymbiont, including bacterial production of succinoglycan, also known as exopolysaccharide-I (EPS-I). InS. melilotiEPS-I production is controlled by the conserved ExoS-ChvI two-component system. Periplasmic ExoR associates with the ExoS histidine kinase and negatively regulates ChvI-dependent expression ofexogenes, necessary for EPS-I synthesis. We show that two extracytoplasmic proteins, LppA (a lipoprotein) and JspA (a metalloprotease), jointly influence EPS-I synthesis by modulating the ExoR-ExoS-ChvI pathway and expression of genes in the ChvI regulon. Deletions ofjspAandlppAled to lower EPS-I production and competitive disadvantage during host colonization, for bothS. melilotiwithMedicago sativaandS. medicaewithM. truncatula. Overexpression ofjspAreduced steady-state levels of ExoR, suggesting that the JspA protease participates in ExoR degradation. This reduction in ExoR levels is dependent on LppA and can be replicated with ExoR, JspA, and LppA expressed exogenously inCaulobacter crescentusandEscherichia coli. Akin to signaling pathways that sense extracytoplasmic stress in other bacteria, JspA and LppA may monitor periplasmic conditions during interaction with the plant host to adjust accordingly expression of genes that contribute to efficient symbiosis. The molecular mechanisms underlying host colonization in our model system may have parallels in related alpha-proteobacteria.Author summarySymbiotic bacteria that live in the roots of legume plants produce biologically accessible nitrogen compounds, offering a more sustainable and environmentally sound alternative to industrial fertilizers generated from fossil fuels. Understanding the multitude of factors that contribute to successful interaction between such bacteria and their plant hosts can help refine strategies for improving agricultural output. In addition, because disease-causing microbes share many genes with these beneficial bacteria, unraveling the cellular mechanisms that facilitate host invasion can reveal ways to prevent and treat infectious diseases. In this report we show that two genes in the model bacteriumSinorhizobium meliloticontribute to effective symbiosis by helping the cells adapt to living in host plants. This finding furthers knowledge about genetics factors that regulate interactions between microbes and their hosts.

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“…In the study of Cazzola et al [ 41 ], a close correlation was observed between the increase in E. coli adhesion to eukaryotic cells and the decrease in cell membrane fluidity of these bacteria. In another study carried out on four selected bacterial species, it was shown that, together with the entry into the biofilm (sedentary) phase, microorganisms accumulate saturated fatty acids in their cell membranes, leading to a decrease in their membranes’ fluidity [ 42 ]. Identical observations were made in another article [ 43 ], where, using Pseudomonas aeruginosa as a model organism, the participation of extracellular vesicles in membrane stiffening and promotion of biofilm formation was additionally noticed.…”

Section: Discussionmentioning

confidence: 99%

Ether Derivatives of Naringenin and Their Oximes as Factors Modulating Bacterial Adhesion

et al. 2023

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Because of the close connection between adhesion and many vital cellular functions, the search for new compounds modulating the adhesion of bacteria belonging to the intestinal microbiota is a great challenge and a clinical need. Based on our previous studies, we discovered that O-lkyl naringenin derivatives and their oximes exhibit antimicrobial activity against antibiotic-resistant pathogens. The current study was aimed at determining the modulatory effect of these compounds on the adhesion of selected representatives of the intestinal microbiota: Escherichia coli, a commensal representative of the intestinal microbiota, and Enterococcus faecalis, a bacterium that naturally colonizes the intestines but has disease-promoting potential. To better reflect the variety of real-life scenarios, we performed these studies using two different intestinal cell lines: the physiologically functioning (“healthy”) 3T3-L1 cell line and the disease-mimicking, cancerous HT-29 line. The study was performed in vitro under static and microfluidic conditions generated by the Bioflux system. We detected the modulatory effect of the tested O-alkyl naringenin derivatives on bacterial adhesion, which was dependent on the cell line studied and was more significant for E. coli than for E. faecalis. In addition, it was noticed that this activity was affected by the concentration of the tested compound and its structure (length of the carbon chain). In summary, O-alkyl naringenin derivatives and their oximes possess a promising modulatory effect on the adhesion of selected representatives of the intestinal microbiota.

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The Fluidity of the Bacterial Outer Membrane Is Species Specific

Cited by 13 publications

References 72 publications

An Outer Membrane Vesicle‐Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability

An Outer Membrane Vesicle‐Based Permeation Assay (OMPA) for Assessing Bacterial Bioavailability

A protease and a lipoprotein jointly modulate the conserved ExoR-ExoS-ChvI signaling pathway critical inSinorhizobium melilotifor symbiosis with legume hosts

Ether Derivatives of Naringenin and Their Oximes as Factors Modulating Bacterial Adhesion

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