The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity

Kahar, Prihardi; Itomi, Akiho; Tsuboi, Hikari; Ishizaki, Miki; Yasuda, Misa; Otsuka, Hiromi; Azmi, Nurlina binti; Matsumoto, Hana; Kondo, Akihiko

doi:10.1016/j.ymben.2022.03.001

Cited by 12 publications

(16 citation statements)

References 54 publications

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“…Also, the ratio of lactic acid to ethanol was remarkably improved (2.67 at 15 h) compared with cultivation using a YPD medium (0.91 at 9 h). This result indicates that the stress of chemical inhibitors at up to certain levels could instead provide a positive impact to the fermentation by shifting the metabolism and restricting the cell growth 24 , which resulted in an increased lactic acid accumulation.…”

Section: Resultsmentioning

confidence: 99%

“…Since acetic acid, formic acid, furfural and levulinic acid are the most common by-products generated during the pretreatment of various lignocellulosic biomass, these chemicals were added to the medium of fermentation to assess the robustness of both strains. The effect of each chemical inhibitor on cell growth was studied at various levels, viz., 0%, 10%, 20%, 40%, 60%, 80% and 100%, in corresponding with the original concentrations of inhibitory chemical complexes (ICC) examined elsewhere 23,24 . BTCC3 strain remarkably grew faster under all four chemicals, including acetic acid, formic acid, furfural and levulinic acid, compared to BY4741 strains.…”

Section: Resultsmentioning

confidence: 99%

“…For instance, in all mutants, the accumulations of by-products, such as ethanol and glycerol, were higher than in wild-type strain, which could have been because of the response of the microbial host to cope with higher acid accumulations. In fact, the accumulations of ethanol and glycerol are known to induce the generation of NAD + , which has an essential role in countering the negative impact of various stressors in cells 24,42,43 . Also, our results revealed that inserting an additional copy of the LDH gene into www.nature.com/scientificreports/ the same locus does not necessarily improve lactic acid production.…”

Section: Discussionmentioning

confidence: 99%

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Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

Pangestu

Kahar

Kholida

et al. 2022

Sci Rep

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Acidic and chemical inhibitor stresses undermine efficient lactic acid bioproduction from lignocellulosic feedstock. Requisite coping treatments, such as detoxification and neutralizing agent supplementation, can be eliminated if a strong microbial host is employed in the process. Here, we exploited an originally robust yeast, Saccharomyces cerevisiae BTCC3, as a production platform for lactic acid. This wild-type strain exhibited a rapid cell growth in the presence of various chemical inhibitors compared to laboratory and industrial strains, namely BY4741 and Ethanol-red. Pathway engineering was performed on the strain by introducing an exogenous LDH gene after disrupting the PDC1 and PDC5 genes. Facilitated by this engineered strain, high cell density cultivation could generate lactic acid with productivity at 4.80 and 3.68 g L−1 h−1 under semi-neutralized and non-neutralized conditions, respectively. Those values were relatively higher compared to other studies. Cultivation using real lignocellulosic hydrolysate was conducted to assess the performance of this engineered strain. Non-neutralized fermentation using non-detoxified hydrolysate from sugarcane bagasse as a medium could produce lactic acid at 1.69 g L−1 h−1, which was competitive to the results from other reports that still included detoxification and neutralization steps in their experiments. This strategy could make the overall lactic acid bioproduction process simpler, greener, and more cost-efficient.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

Pangestu

Kahar

Kholida

et al. 2022

Sci Rep

Self Cite

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“…( Klis, 2002 ). Increased cell wall hydrophobicity influences flocculation leading to increased robustness to inhibitory chemical compounds ( Kahar et al, 2022 ). This was associated with changes in the expression of MOT3 gene, encoding a transcription regulator that controls the expression of a cell wall protein (CWPs) encoding gene, YGP1 , that influences cell wall hydrophobicity ( Kahar et al, 2022 ).…”

Section: The Yeast Cell Wall: Composition Structure Function and Bios...mentioning

confidence: 99%

The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts

2022

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In industrial settings and processes, yeasts may face multiple adverse environmental conditions. These include exposure to non-optimal temperatures or pH, osmotic stress, and deleterious concentrations of diverse inhibitory compounds. These toxic chemicals may result from the desired accumulation of added-value bio-products, yeast metabolism, or be present or derive from the pre-treatment of feedstocks, as in lignocellulosic biomass hydrolysates. Adaptation and tolerance to industrially relevant stress factors involve highly complex and coordinated molecular mechanisms occurring in the yeast cell with repercussions on the performance and economy of bioprocesses, or on the microbiological stability and conservation of foods, beverages, and other goods. To sense, survive, and adapt to different stresses, yeasts rely on a network of signaling pathways to modulate the global transcriptional response and elicit coordinated changes in the cell. These pathways cooperate and tightly regulate the composition, organization and biophysical properties of the cell wall. The intricacy of the underlying regulatory networks reflects the major role of the cell wall as the first line of defense against a wide range of environmental stresses. However, the involvement of cell wall in the adaptation and tolerance of yeasts to multiple stresses of biotechnological relevance has not received the deserved attention. This article provides an overview of the molecular mechanisms involved in fine-tuning cell wall physicochemical properties during the stress response of Saccharomyces cerevisiae and their implication in stress tolerance. The available information for non-conventional yeast species is also included. These non-Saccharomyces species have recently been on the focus of very active research to better explore or control their biotechnological potential envisaging the transition to a sustainable circular bioeconomy.

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“…Microbial attachment to surfaces is known to be impacted by variation in cell surface properties, such as hydrophobicity and the presence and abundance of various proteins and sugars [ 1 ]. Some of these same cell surface properties have also been implicated in microbial tolerance to harsh growth conditions [ 2 – 4 ]. It is desirable to be able to predictably engineer the properties of the microbial cell membrane for biotechnology applications [ 2 , 5 – 7 ].…”

Section: Introductionmentioning

confidence: 99%

Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution

Liao

Santoscoy²,

Craft³

et al. 2022

PLoS ONE

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Outer membrane protein A (OmpA) is one of the most abundant outer membrane proteins of Gram-negative bacteria and is known to have patterns of sequence variations at certain amino acids—allelic variation—in Escherichia coli. Here we subjected seven exemplar OmpA alleles expressed in a K-12 (MG1655) ΔompA background to further characterization. These alleles were observed to significantly impact cell surface charge (zeta potential), cell surface hydrophobicity, biofilm formation, sensitivity to killing by neutrophil elastase, and specific growth rate at 42°C and in the presence of acetate, demonstrating that OmpA is an attractive target for engineering cell surface properties and industrial phenotypes. It was also observed that cell surface charge and biofilm formation both significantly correlate with cell surface hydrophobicity, a cell property that is increasingly intriguing for bioproduction. While there was poor alignment between the observed experimental values relative to the known sequence variation, differences in hydrophobicity and biofilm formation did correspond to the identity of residue 203 (N vs T), located within the proposed dimerization domain. The relative abundance of the (I, δ) allele was increased in extraintestinal pathogenic E. coli (ExPEC) isolates relative to environmental isolates, with a corresponding decrease in (I, α) alleles in ExPEC relative to environmental isolates. The (I, α) and (I, δ) alleles differ at positions 203 and 251. Variations in distribution were also observed among ExPEC types and phylotypes. Thus, OmpA allelic variation and its influence on OmpA function warrant further investigation.

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The flocculant Saccharomyces cerevisiae strain gains robustness via alteration of the cell wall hydrophobicity

Cited by 12 publications

References 54 publications

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

Harnessing originally robust yeast for rapid lactic acid bioproduction without detoxification and neutralization

The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts

Allelic variation of Escherichia coli outer membrane protein A: Impact on cell surface properties, stress tolerance and allele distribution

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