The FlexISH assay brings flexibility to cytogenetic HER2 testing

Abstract

The results obtained with both the short-run and long-run application of FlexISH are in excellent accordance with the results obtained with other commercially available FISH kits. This appears to be true in all relevant respects: signal counts, signal-to-noise ratio, brightness, and distinctness of HER2 and cen17 signals. As FlexISH probes can be equivalently used as a short-run or long-run application, the FlexISH probe kit provides the highest flexibility in terms of time and laboratory management. If required, a reliable HER2 finding can be delivered within 4.5 h, but the standard workflow (including overnight hybridization) does not negatively affect the performance, specimen quality or diagnostic result.

The presence of PD-1 positive tumor infiltrating lymphocytes in triple negative breast cancers is associated with a favorable outcome of disease

“…All specimens were acquired from the tissue archive of the Institute of Pathology, University of Regensburg (Germany). The embedding procedure was performed as described elsewhere [ 44 ]. Immediately after surgery, the breast tissues were transferred into the formalin fixative (4% formaldehyde, 1% sodium phosphate; SG Planung, Holzkirchen, Germany).…”

Association Between Contrast‐Enhanced Ultrasound Characteristics and Molecular Subtypes of Breast Cancer

“…HER2(2+) was considered a marginal result, and fluorescence in situ hybridization was used to detect amplification of the HER2 gene; if no HER2 amplification was found, HER2 (2+) was classified as HER2-negative, and if HER2 amplification was detected, HER2(2+) was listed as HER2-positive. 29 The cut-off point for ER-positivity was 10% and the cut-off for PR-positivity was 20%. The cutoff point between "high" and "low" Ki67 values varied between laboratories, and we used 14% as the threshold.…”

HER2FISHresults in breast cancers with increasedCEN17 signals using alternative chromosome 17 probes – reclassifying cases in the equivocal category

“…All samples of the HER2 amplified collective were IHC 3 + . Tissue microarrays (TMAs) were generated for the FISH analyses as described previously . In brief, for each tumour a representative tumour section was selected from a haematoxylin and eosin (H&E)‐stained section of the donor block.…”

“…Tissue microarrays (TMAs) were generated for the FISH analyses as described previously. [16][17][18] In brief, for each tumour a representative tumour section was selected from a haematoxylin and eosin (H&E)-stained section of the donor block. The relevant tissue section was identified by a pathologist based on tissue architecture, morphology and HER2-IHC.…”