The flexible feedstock concept in Industrial Biotechnology: Metabolic engineering of Escherichia coli, Corynebacterium glutamicum, Pseudomonas, Bacillus and yeast strains for access to alternative carbon sources

Wendisch, Volker F.; Brito, Luciana Fernandes de; López, Marina Gil; Hennig, Guido; Pfeifenschneider, Johannes; Sgobba, Elvira; Veldmann, Kareen H.

doi:10.1016/j.jbiotec.2016.07.022

Cited by 100 publications

(79 citation statements)

References 190 publications

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“…Due to insufficient global food supply, the use of feedstocks, which can primarily be used also for food production, is at least ethically questionable and not a preferable basis for the establishment of a truly sustainable bioeconomy. Nevertheless, numerous current biotechnological production processes mostly depend on glucose as carbon source (Wendisch et al, ). The conflict between food and biotechnology and the resulting demand to create ethically less problematic processes, which also offer a promising potential for increasing positive socio‐economical perception and acceptance by customers of biotechnological products, alternative carbon sources like lignocellulosic biomass, have moved into the focus of attention as renewable and thus sustainable raw materials with a considerable economic potential for industrial biotechnology.…”

Section: Introductionmentioning

confidence: 99%

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose, and arabinose: Hemicellulose hydrolysates and their major sugars as sustainable carbon sources

et al. 2019

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Lignocellulosic biomass is the most abundant bioresource on earth containing polymers mainly consisting of d‐glucose, d‐xylose, l‐arabinose, and further sugars. In order to establish this alternative feedstock apart from applications in food, we engineered Pseudomonas putida KT2440 as microbial biocatalyst for the utilization of xylose and arabinose in addition to glucose as sole carbon sources. The d‐xylose‐metabolizing strain P. putida KT2440_xylAB and l‐arabinose‐metabolizing strain P. putida KT2440_araBAD were constructed by introducing respective operons from Escherichia coli. Surprisingly, we found out that both recombinant strains were able to grow on xylose as well as arabinose with high cell densities and growth rates comparable to glucose. In addition, the growth characteristics on various mixtures of glucose, xylose, and arabinose were investigated, which demonstrated the efficient co‐utilization of hexose and pentose sugars. Finally, the possibility of using lignocellulose hydrolysate as substrate for the two recombinant strains was verified. The recombinant P. putida KT2440 strains presented here as flexible microbial biocatalysts to convert lignocellulosic sugars will undoubtedly contribute to the economic feasibility of the production of valuable compounds derived from renewable feedstock.

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Section: Introductionmentioning

confidence: 99%

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose, and arabinose: Hemicellulose hydrolysates and their major sugars as sustainable carbon sources

et al. 2019

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“…However, the general physiological response of C. glutamicum to complex medium (compounds) was not studied yet. To date, supplementation investigations with the strain mostly aimed at media optimization (Coello et al, 2002; Jeon et al, 2013) or usage of alternative carbon and/or nitrogen sources (Lee et al, 2014; Wendisch et al, 2016; Lange et al, 2017). For E. coli , in contrast, profound knowledge exists based on physiological investigations in Luria–Bertani (LB) broth (Sezonov et al, 2007) and due to comparable transcriptional expression analyses of late-exponential E. coli cultures grown in minimal and LB medium (Tao et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions

et al. 2018

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To ensure economic competitiveness, bioprocesses should achieve maximum productivities enabled by high growth rates (μ) and equally high substrate consumption rates (qS) as a prerequisite of sufficient carbon-to-product conversion. Both traits were investigated and improved via bioprocess engineering approaches studying the industrial work horse Corynebacterium glutamicum. Standard minimal medium CGXII with glucose as sole carbon source was supplemented with complex brain-heart-infusion (BHI) or amino acid (AA) cocktails. Maximum μ of 0.67 h-1 was exclusively observed in 37 g BHI L-1 whereas only minor growth stimulation was found after AA supplementation (μ = 0.468 h-1). Increasing glucose consumption rates (qGlc) were solely observed in certain dosages of BHI (1–10 g L-1), while 37 g BHI L-1 and AA addition revealed qGlc below the reference experiments. Moreover, BHI supplementation revealed Monod-type saturation kinetics of μ (KBHI = 2.73 g BHI L-1) referring to the preference of non-AAs as key boosting nutrients. ATP-demands under reference, 1 g BHI L-1, and AA conditions were nearly constant but halved in BHI concentrations above 5 g L-1 reflecting the energetic advantage of consuming complex nutrient components in addition to “simple” building blocks such as AAs. Furthermore, C. glutamicum revealed maximum biomass per carbon yields of about 18 gCDW C-mol-1 irrespective of the medium. In AA supplementation experiments, simultaneous uptake of 17 AAs was observed, maximum individual consumption rates determined, and L-asparagine and L-glutamine were distinguished as compounds with the highest consumption rates. Employment of the expanded stoichiometric model iMG481 successfully reproduced experimental results and revealed the importance of C. glutamicum’s transaminase network to compensate needs of limiting AA supply. Model-based sensitivity studies attributed the highest impact on μ to AAs with high ATP and NADPH demands such as L-tryptophan or L-phenylalanine.

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“…The (somewhat short) list of bacterial hosts falling into this category includes Escherichia coli (Pontrelli et al ., ), Bacillus subtilis (Gu et al ., ), Streptomyces sp. (Spasic et al ., ), Pseudomonas putida (Nikel and de Lorenzo, ), and Corynebacterium glutamicum (Wendisch et al ., ), for which extensive background fundamental knowledge has been amassed. The wide use of these well‐established chassis notwithstanding, there has been a renewed interest for bringing up‐and‐coming host alternatives to the metabolic engineering community, thus broadening the repertoire of chassis available.…”

Section: Desirable Properties In the Ideal Bacterial Chassis: Bridginmentioning

confidence: 99%

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

Calero

Nikel

2018

Microbial Biotechnology

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The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful of bacteria have attained the acceptance and widespread use that are needed to fulfil the needs of industrial bioproduction - and only for the synthesis of very few, structurally simple compounds. One of the reasons for this unfortunate circumstance has been the dearth of tools for targeted genome engineering of bacterial chassis, and, nowadays, synthetic biology is significantly helping to bridge such knowledge gap. Against this background, in this review, we discuss the state of the art in the rational design and construction of robust bacterial chassis for metabolic engineering, presenting key examples of bacterial species that have secured a place in industrial bioproduction. The emergence of novel bacterial chassis is also considered at the light of the unique properties of their physiology and metabolism, and the practical applications in which they are expected to outperform other microbial platforms. Emerging opportunities, essential strategies to enable successful development of industrial phenotypes, and major challenges in the field of bacterial chassis development are also discussed, outlining the solutions that contemporary synthetic biology-guided metabolic engineering offers to tackle these issues.

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The flexible feedstock concept in Industrial Biotechnology: Metabolic engineering of Escherichia coli, Corynebacterium glutamicum, Pseudomonas, Bacillus and yeast strains for access to alternative carbon sources

Cited by 100 publications

References 190 publications

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose, and arabinose: Hemicellulose hydrolysates and their major sugars as sustainable carbon sources

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose, and arabinose: Hemicellulose hydrolysates and their major sugars as sustainable carbon sources

Physiological Response of Corynebacterium glutamicum to Increasingly Nutrient-Rich Growth Conditions

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

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