The flavonols quercetin and 3′,4′-dihydroxyflavonol reduce platelet function and delay thrombus formation in a model of type 1 diabetes

Mosawy, Sapha; Jackson, Denise E.; Woodman, Owen L.; Linden, Matthew D.

doi:10.1177/1479164114524234

Cited by 21 publications

(20 citation statements)

References 47 publications

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“…including in vitro platelet activation, in vivo thrombosis inhibition [21][22][23][24] and human interventional studies on CVDs [25][26][27][28] of quercetin, and in vitro platelet activation inhibition [29] of quercetin-3-O-β-D-glucoside have been reported in dozens of research papers, few or no reports exist on the in vitro actions of quercetin and quercetin-3-O-β-D-glucoside on procoagulant proteinases, fibrin polymer formation, and blood clot, the in vitro and ex vivo anticoagulation actions of quercetin and quercetin-3-O-β-D-glucoside, or the in vivo effects of quercetin and quercetin-3-O-β-D-glucoside against arterial thrombosis and acute thromboembolism models and platelet activation.…”

Section: Introductionmentioning

confidence: 99%

Comparative Effect of Quercetin and Quercetin‐3‐O‐β‐d‐Glucoside on Fibrin Polymers, Blood Clots, and in Rodent Models

Choi

Kim

2016

J Biochem & Molecular Tox

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The present study evaluates the in vitro, in vivo, and ex vivo antithrombotic and anticoagulant effect of two flavonoids: quercetin and quercetin-3-O-β-d-glucoside (isoquercetin). The present results have shown that quercetin and isoquercetin inhibit the enzymatic activity of thrombin and FXa and suppress fibrin clot formation and blood clotting. The prolongation effect of quercetin and isoquercetin against epinephrine and collagen-induced platelet activation may have been caused by intervention in intracellular signaling pathways including coagulation cascade and aggregation response on platelets and blood. The in vivo and ex vivo anticoagulant efficacy of quercetin and isoquercetin was evaluated in thrombin-induced acute thromboembolism model and in ICR mice. Our findings showed that in vitro and in vivo inhibitory effects of quercetin were slightly higher than that of quercetin glucoside, whereas in vitro and ex vivo anticoagulant effects of quercetin were weaker than that of quercetin glucoside because of their structural characteristics.

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Section: Introductionmentioning

confidence: 99%

Comparative Effect of Quercetin and Quercetin‐3‐O‐β‐d‐Glucoside on Fibrin Polymers, Blood Clots, and in Rodent Models

Choi

Kim

2016

J Biochem & Molecular Tox

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“…Quercetin is well known for its beneficial cardiovascular properties. It has been demonstrated to be effective in reducing or inhibiting platelet aggregation and activation in many studies, although the exact mechanism is not fully understood [20,21,39]. However, quercetin has a poor aqueous solubility, and cannot be directly immobilized onto the surface of biomaterials.…”

Section: Curves D and E Inmentioning

confidence: 99%

“…The coagulation assays conducted by Choi J H et al also demonstrated that quercetin inhibits the enzymatic activity of thrombin and FXa, and suppresses fibrin clot formation and blood clotting [19]. Actually, the antithrombotic effects of quercetin, including the inhibition of the platelet activation and aggregation, in vivo thrombosis inhibition, and human interventional studies on cardiovascular diseases, have been widely reported [20][21][22][23][24]. Furthermore, quercetin has other pharmacological functions, such as antioxidation [25], anti-inflammation [26], and the inhibition of smooth muscle cell proliferation and migration [27].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-Layer Deposition of Hyaluronan and Quercetin-Loaded Chitosan Nanoparticles onto Titanium for Improving Blood Compatibility

Liu

Chen

et al. 2020

Coatings

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Surface modification is an effective way to improve the hemocompatibility of biomaterials. Quercetin has significant anticoagulation and antithrombotic effects, and thus it is a promising candidate agent for the surface modification of blood-contacting materials. In this study, quercetin was successfully encapsulated in tripolyphosphate–chitosan nanoparticles (TCs) based on the ionic gelation of chitosan with tripolyphosphate (TPP) anions. Then, hyaluronan acid (HA)/quercetin-loaded TPP–chitosan nanoparticle (QTCs) films, in addition to HA/TCs films, were prepared separately using an electrostatic layer-by-layer self-assembly technique. The encapsulation of quercetin in the chitosan nanoparticles was confirmed by UV spectra. The quercetin-loaded multilayer coatings were also successfully self-assembled, as confirmed by the UV spectra and contact angle measurements. Platelet adhesion experiments were carried out with platelet-enriched plasma so as to evaluate the blood compatibility of the different samples. There were many platelets on the surfaces of the glass and HA/TC-coated titanium, which were partially activated but not aggregated. Meanwhile, many more platelets were observed on the uncoated titanium surfaces, most of which developed pseudopodia. By contrast, the platelet adhesion and activation were reduced remarkably on the surface of the HA/QTC-coated titanium. These results showed that the multilayer coatings containing quercetin could act as potential biomaterials to improve the anticoagulation performance of blood-contacting materials.

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“…Ïîòàñêàëîâà Â.Ñ., Õàéòîâè÷ Ì.Â., Ïëºíîâà Î.Ì., Âàë³ãóðà Ì.Ñ. ³íîâèé ïîòåíö³àë, â³äì³÷åíà òåíäåíö³ÿ äî â³äíîâëåííÿ àêòèâíîñò³ ïðîòå¿íó Ñ òà Õ²²-à çàëåaeíîãî ô³áðè-íîë³çó, ùî ïåâíîþ ì³ðîþ óçãîäaeóºòüñÿ ç äàíèìè ³íøèõ äîñë³äíèê³â [7,12].…”

Section: òàáëèöÿ 1 õàðàêòåðèñòèêà ñèñòåìè ãåìîñòàçó ó ùóð³â ð³çíèõ ãðóïunclassified

“…Îòaeå, àíàë³ç îòðèìàíèõ ðåçóëüòàò³â ó ùóð³â ²² ãðóïè âêàçóº íà óïîâ³ëüíåííÿ çãîðòàííÿ êðîâ³, çîêðåìà çà ðàõóíîê óòâîðåííÿ çãóñòêó, çðîñòàííÿ àíòè-òðîìá³íîâî¿ àêòèâíîñò³ êðîâ³ òà â³äíîâëåííÿ àêòèâ-íîñò³ ïðîòå¿íó Ñ (â³äïîâ³äíî íà 39,5% òà 19,3%) òà ïîñèëåííÿ ô³áðèíîë³çó (íà 25%). Çðîñòàííÿ êîíöåí-òðàö³¿ ÐÔÌÊ òà çíèaeåííÿ ð³âíþ ô³áðèíîãåíó â³äîáðàaeàº ïðîòèçàïàëüíèé åôåêò êâåðöåòèíó ïðè íèçüêîìó ðèçèêó êðîâîòå÷ çà óìîâ îäíî÷àñíîãî çàñòîñóâàííÿ ³ç àíòèêîàãóëÿíòàìè [6,9,12].…”

Section: òàáëèöÿ 1 õàðàêòåðèñòèêà ñèñòåìè ãåìîñòàçó ó ùóð³â ð³çíèõ ãðóïunclassified

Thrombose-Preventive Influence of Quercetin in Rats on the Hyper-Coagulation Diet

et al. 2019

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Relevance. Hyper-coagulation processes increase the risk of coronary heart disease. Objective of the study was to study the effect of quercetin on the hemostatic system when using hyper-coagulation diet in rats. Materials and methods. The study included 17 male rats weighing 240-270 g. The rats of the control group (n = 6) were fed with conventional compound feed; Group I rats (n = 5) - mixed feed; and group II rats (n = 6) mixed with meat and quercetin (1.33 g/l). After 21 days, sterile blood was collected. The Amellung Coagulometer KS 4A studied the external and internal blood coagulation pathways, the activity of their own anticoagulant and fibrinolytic blood systems. The data obtained were statistically processed using Excel 2010 and Statistica v.10 (StatSoft, USA). Results. In rats of group 1, the external pathway of blood coagulation underwent the most significant changes, as indicated by an almost four-fold acceleration of prothrombin time and a decrease in the international normalized ratio. From the side of the internal pathway, the greatest changes were recorded in the last phase of clot formation: thrombin time decreased by 39.8% with an almost constant rate of activated partial thromboplastin time (APTT), which confirmed the activation of the fibrin formation phase. In addition, the content of soluble fibrin-monomer complexes (RFMC) increased 1.7 times and the fibrinogen decreased. At the same time, depletion of the anticoagulant potential of the blood was observed (a 42.2% decrease in the activity of the protein C system with a tendency to a decrease in antithrombin III (AT III). The fibrinolytic system was significantly suppressed, as indicated by more than three times inhibition of XII-dependent fibrinolysis. In animals Group 2 compared with the indicator in group 1, APTT was 2 times longer; the concentration of RFMC was also doubled, fibrinolysis was activated by 25%. Conclusion: The use of quercetin bioflavonoids in animals with simulated coronary heart disease slows down blood coagulation and potentiates the anticoagulant system, including due to a 40% increase in AT III activity, can be considered as a thrombosis-preventive effect

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The flavonols quercetin and 3′,4′-dihydroxyflavonol reduce platelet function and delay thrombus formation in a model of type 1 diabetes

Cited by 21 publications

References 47 publications

Comparative Effect of Quercetin and Quercetin‐3‐O‐β‐d‐Glucoside on Fibrin Polymers, Blood Clots, and in Rodent Models

Comparative Effect of Quercetin and Quercetin‐3‐O‐β‐d‐Glucoside on Fibrin Polymers, Blood Clots, and in Rodent Models

Layer-by-Layer Deposition of Hyaluronan and Quercetin-Loaded Chitosan Nanoparticles onto Titanium for Improving Blood Compatibility

Thrombose-Preventive Influence of Quercetin in Rats on the Hyper-Coagulation Diet

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