The flavonoid, kaempferol‐3‐O‐apiofuranosyl‐7‐O‐rhamnopyranosyl, as a potential therapeutic agent for breast cancer with a promoting effect on ovarian function

Abstract

It is widely known that breast cancer cells eventually develop resistance to hormonal drugs and chemotherapies, which often compromise fertility. This study aimed to investigate the effect of the flavonoid, kaempferol-3-O-apiofuranosyl-7-O-rhamnopyranosyl (KARP), on 1) the viability of MCF-7 breast cancer cells and 2) ovarian function in rats.A dose-dependent decrease in MCF-7 cell survival was observed, and the IC50 value was found to be 48 μg/ml. Cells in the control group or those exposed to increasing concentrations of KARP experienced a similar generation of reactive oxygen species and induction of apoptosis. For the rats, estradiol levels correlated negatively to KARP dosages, although a recovery was obtained at administration of 30 mg/kg per day.Noteworthily, when compared against the control, this dosage led to significant increases in mRNA levels for CYP19, CYP17a, CCND2, GDF9, and INSL3 among the treatment groups, and ER1 and ER2 mRNA levels decreased in a dose-dependent manner. KARP shows great promise as an ideal therapy for breast cancer patients since it induced apoptosis and autophagy in cancerous cells without harming fertility in our animal model. Future investigations on humans are necessary to substantiate these findings and determine its efficacy as a general line of treatment.

XGBoost-based and tumor-immune characterized gene signature for the prediction of metastatic status in breast cancer

“…Through comparison each gene of our gene signature with existing studies separately, we found that there are many studies on the association between gene PTGS2, gene SQSTM1 and breast cancer. Researches on gene LINC01125 [ 25 ], gene GDF9 [ 41 , 42 ] and gene GVINP1 [ 43 ] is very limited, no studies have shown that there is a link between gene TMEM64 and breast cancer. Most of the genes we have proposed in this research have been biologically verified, which also proves the reliability of our gene signature.…”

The expression patterns of different cell types and their interactions in the tumor microenvironment are predictive of breast cancer patient response to neoadjuvant chemotherapy

“…6E) with roles in reproductive biology and signal transduction [74][75][76] [77], emerged as a key CCI with the same directionality across multiple cell types. These genes have been implicated in BC prognosis and therapeutic outcomes [78] [79][80], with BMPR1B exhibiting dual roles in chemotherapy response [81][82][83]. Another top CCI involved the renowned GDNF -RET signaling pathway interactions between 'NRTN' (neurturin), or its paralog 'ARTN' (artemin), and 'GFRA1' (GDNF family receptor alpha 1) in multiple cell types (Fig.…”

The role of kaempferol in gynaecological malignancies: progress and perspectives

“…Furthermore, KV triggers the overproduction of ROS by upregulating LC3-II and p62, leading to autophagy-mediated cell death. Harrath et al (2021) discovered that kaempferol-3-O-apiofuranosyl-7-O-rhamnopyranosyl can enhance ROS production, inducing dose-dependent autophagy and apoptosis of MCF-7 breast cancer cells. The findings suggest that the compound may be a promising treatment for breast cancer patients ( Figure 4 ).…”