The flame photometric determination of sodium, potassium and calcium in plant extracts with special reference to interference effects

Havre, Gustav N.

doi:10.1016/s0003-2670(01)81614-7

Cited by 27 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the estimation of potassium (K) and calcium (Ca) contents in rice leaves, 1 g of dried leaf sample was digested in 12 mL of a perchloric acid, sulfuric acid, and nitric acid (1:2:9) mixture for 3 h in a cold bath and 3 h in a sand bath until complete digestion [ 72 ]. The concentration (ppm g −1 DW) of K was measured after the neutralization of digestate with ammonium hydroxide using a flame photometer equipped with a K lamp at 766 nm [ 73 ]. The Ca concentration (%) was estimated using the titration method proposed by Tucker and Kurtz (1961) [ 74 ].…”

Section: Methodsmentioning

confidence: 99%

The Foliar Application of Rice Phyllosphere Bacteria induces Drought-Stress Tolerance in Oryza sativa (L.)

Devarajan

Muthukrishanan

Truu

et al. 2021

Plants

View full text Add to dashboard Cite

: This study assessed the potential of Bacillus endophyticus PB3, Bacillus altitudinis PB46, and Bacillus megaterium PB50 to induce drought tolerance in a susceptible rice cultivar. The leaves of the potted rice plants subjected to physical drought stress for 10 days during the flowering stage were inoculated with single-strain suspensions. Control pots of irrigated and drought-stressed plants were included in the experiment for comparison. In all treatments, the plant stress-related physiochemical and biochemical changes were examined and the expression of six stress-responsive genes in rice leaves was evaluated. The colonization potential on the surface of the rice leaves and stomata of the most successful strain in terms of induced tolerance was confirmed in the gnotobiotic experiment. The plants sprayed with B. megaterium PB50 showed an elevated stress tolerance based on their higher relative water content and increased contents of total sugars, proteins, proline, phenolics, potassium, calcium, abscisic acid, and indole acetic acid, as well as a high expression of stress-related genes (LEA, RAB16B, HSP70, SNAC1, and bZIP23). Moreover, this strain improved yield parameters compared to other treatments and also confirmed its leaf surface colonization. Overall, this study indicates that the foliar application of B. megaterium PB50 can induce tolerance to drought stress in rice.

show abstract

Section: Methodsmentioning

confidence: 99%

The Foliar Application of Rice Phyllosphere Bacteria induces Drought-Stress Tolerance in Oryza sativa (L.)

Devarajan

Muthukrishanan

Truu

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…2.6. Determination of Na, K and Ca Leaf mineral concentrations of Na, K and Ca were determined using the flame photometric method (Jenway, Staffordshire, UK) as described by Havre [37].…”

Section: Assay Of Antioxidant Enzymesmentioning

confidence: 99%

Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes

Youssef

Raafat

El-Yazied

et al. 2021

Plants

View full text Add to dashboard Cite

In plants, α-Lipoic acid (ALA) is considered a dithiol short-chain fatty acid with several strong antioxidative properties. To date, no data are conclusive regarding its effects as an exogenous application on salt stressed sorghum plants. In this study, we investigated the effect of 20 µM ALA as a foliar application on salt-stressed sorghum plants (0, 75 and 150 mM as NaCl). Under saline conditions, the applied-ALA significantly (p ≤ 0.05) stimulated plant growth, indicated by improving both fresh and dry shoot weights. A similar trend was observed in the photosynthetic pigments, including Chl a, Chl b and carotenoids. This improvement was associated with an obvious increase in the membrane stability index (MSI). At the same time, an obvious decrease in the salt induced oxidative damages was seen when the concentration of H2O2 and malondialdehyde (MDA) was reduced in the salt stressed leaf tissues. Generally, ALA-treated plants demonstrated higher antioxidant enzyme activity than in the ALA-untreated plants. A moderate level of salinity (75 mM) induced the highest activities of superoxide dismutase (SOD), guaiacol peroxidase (G-POX), and ascorbate peroxidase (APX). Meanwhile, the highest activity of catalase (CAT) was seen with 150 mM NaCl. Interestingly, applied-ALA led to a substantial decrease in the concentration of both Na and the Na/K ratio. In contrast, K and Ca exhibited a considerable increase in this respect. The role of ALA in the regulation of K+/Na+ selectivity under saline condition was confirmed through a molecular study (RT-PCR). It was found that ALA treatment downregulated the relative gene expression of plasma membrane (SOS1) and vacuolar (NHX1) Na+/H+ antiporters. In contrast, the high-affinity potassium transporter protein (HKT1) was upregulated.

show abstract

“…The leaf, stem, root, and total dry weight data were recorded. Dry weight of the seedlings was determined by oven-drying the seedlings at 80 • C for 3 days, and the percentages of Na+ and K+ in the leaves, stems, and roots also were determined, according to the flame photometric method [39]. In addition, the percentages of Na+ and K+ in the leaves, stems, and roots also were determined, according to the flame photometric method [39].…”

Section: Evaluation Of Salt Tolerance In the Bc 4 F 4 Populations (Exp 4)mentioning

confidence: 99%

“…Dry weight of the seedlings was determined by oven-drying the seedlings at 80 • C for 3 days, and the percentages of Na+ and K+ in the leaves, stems, and roots also were determined, according to the flame photometric method [39]. In addition, the percentages of Na+ and K+ in the leaves, stems, and roots also were determined, according to the flame photometric method [39]. In brief, pounding sample volume 0.5 g of each tissues were digest by 10 mL nitric acid (HNO 3 ) and 5 mL perchloric acid (HClO 4 ), then incubated at 200 • C. The contents were covered to reflux acid fumes generated during digestion until digest appeared translucent.…”

Section: Evaluation Of Salt Tolerance In the Bc 4 F 4 Populations (Exp 4)mentioning

confidence: 99%

Improvement of a RD6 Rice Variety for Blast Resistance and Salt Tolerance through Marker-Assisted Backcrossing

et al. 2020

View full text Add to dashboard Cite

RD6 is one of the most favorable glutinous rice varieties consumed throughout the north and northeast of Thailand because of its aroma and softness. However, blast disease and salt stress cause decreases in both yield quantity and quality during cultivation. Here, gene pyramiding via marker-assisted backcrossing (MAB) using combined blast resistance QTLs (qBl 1, 2, 11, and 12) and Saltol QTL was employed in solving the problem. To pursue our goal, the RD6 introgression line (RGD07005-12-165-1), containing four blast-resistant QTLs, were crossed with the Pokkali salt tolerant variety. Blast resistance evaluation was thoroughly carried out in the fields, from BC2F2:3 to BC4F4, using the upland short-row and natural field infection methods. Additionally, salt tolerance was validated in both greenhouse and field conditions. We found that the RD6 “BC4F4 132-12-61” resulting from our breeding programme successfully resisted blast disease and tolerated salt stress, while it maintained the desirable agronomic traits of the original RD6 variety. This finding may provide a new improved rice variety to overcome blast disease and salt stress in Northeast Thailand.

show abstract

The flame photometric determination of sodium, potassium and calcium in plant extracts with special reference to interference effects

Cited by 27 publications

References 12 publications

The Foliar Application of Rice Phyllosphere Bacteria induces Drought-Stress Tolerance in Oryza sativa (L.)

The Foliar Application of Rice Phyllosphere Bacteria induces Drought-Stress Tolerance in Oryza sativa (L.)

Exogenous Application of Alpha-Lipoic Acid Mitigates Salt-Induced Oxidative Damage in Sorghum Plants through Regulation Growth, Leaf Pigments, Ionic Homeostasis, Antioxidant Enzymes, and Expression of Salt Stress Responsive Genes

Improvement of a RD6 Rice Variety for Blast Resistance and Salt Tolerance through Marker-Assisted Backcrossing

Contact Info

Product

Resources

About