The flagellin of Vibrio parahaemolyticus induces the inflammatory response of Tetraodon nigroviridis through TLR5M

Yu, Xue; Xu, Jiachang; Zhao, Yulin; Lu, Yuyou; Deng, Niuniu; Lin, Haoran; Zhang, Yong; Lu, Danqi

doi:10.1016/j.fsi.2021.10.024

Cited by 8 publications

(2 citation statements)

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“…According to the statistics of China Fisheries Yearbook, the farming output of grouper in 2020 has reached 192000 tons, ranking third in China's mariculture fish, creating huge economic and social benefits. With the rapid development of intensive large-scale aquaculture in recent years, bacterial infections continue to break out and spread (1). Vibrio parahaemolyticus, a Gram-negative, halophilic bacterium, inhabits marine and estuarine environment (2).…”

Section: Introductionmentioning

confidence: 99%

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Integrative analyses of mRNA and microRNA expression profiles reveal the innate immune mechanism for the resistance to Vibrio parahaemolyticus infection in Epinephelus coioides

Qiao

et al. 2022

Front. Immunol.

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Vibrio parahaemolyticus, as one of the main pathogens of marine vibriosis, has brought huge losses to aquaculture. However, the interaction mechanism between V. parahaemolyticus and Epinephelus coioides remains unclear. Moreover, there is a lack of comprehensive multi-omics analysis of the immune response of grouper spleen to V. parahaemolyticus. Herein, E. coioides was artificially injected with V. parahaemolyticus, and it was found that the mortality was 16.7% in the early stage of infection, and accompanied by obvious histopathological lesions in the spleen. Furthermore, 1586 differentially expressed genes were screened by mRNA-seq. KEGG analysis showed that genes were significantly enriched in immune-related pathways, Acute-phase immune response, Apoptosis, Complement system and Cytokine-cytokine receptor interaction. As for miRNA-seq analysis, a total of 55 significantly different miRNAs were identified. Further functional annotation analysis indicated that the target genes of differentially expressed miRNAs were enriched in three important pathways (Phosphatidylinositol signaling system, Lysosome and Focal adhesions). Through mRNA-miRNA integrated analysis, 1427 significant miRNA–mRNA pairs were obtained and “p53 signaling pathway”, “Intestinal immune network for IgA production” were considered as two crucial pathways. Finally, miR-144-y, miR-497-x, novel-m0459-5p, miR-7133-y, miR-378-y, novel-m0440-5p and novel-m0084-3p may be as key miRNAs to regulate immune signaling pathways via the miRNA-mRNA interaction network. The above results suggest that the mRNA-miRNA integrated analysis not only sheds new light on the molecular mechanisms underlying the interaction between host and V. parahaemolyticus but also provides valuable and new insights into resistance to vibrio infection.

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Section: Introductionmentioning

confidence: 99%

“…However, the interaction mechanism between V. parahaemolyticus and host has not yet been elucidated. The innate immune system provides the first line of defense against microbial invasion (1). Thus, many fish could respond to pathogens and then eliminated pathogens (8-10).…”

Section: Introductionmentioning

confidence: 99%