2007
DOI: 10.1110/ps.062639307
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The first step of hen egg white lysozyme fibrillation, irreversible partial unfolding, is a two‐state transition

Abstract: Amyloid fibril depositions are associated with many neurodegenerative diseases as well as amyloidosis. The detailed molecular mechanism of fibrillation is still far from complete understanding. In our previous study of in vitro fibrillation of hen egg white lysozyme, an irreversible partially unfolded intermediate was characterized. A similarity of unfolding kinetics found for the secondary and tertiary structure of lysozyme using deep UV resonance Raman (DUVRR) and tryptophan fluorescence spectroscopy leads t… Show more

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Cited by 65 publications
(71 citation statements)
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References 112 publications
(126 reference statements)
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“…It has been previously reported that the random component of the secondary structure plays a relevant role in the fibrillation process, as in the first stage of the fibrillation the native structure falls in a disordered structure and then develops into an organized β inter-chain structure. 62 It is important to recall that the peak at around 1670 cm -1 is the marker of ordered β sheet structures in the aggregated sample, and that this feature together with the narrowing of the amide I band are the most clear markers of fibril formation in the Raman spectra. 63 Shifts at lower frequencies can be observed in the amide III region coherently with the above mentioned changes in the secondary structure from α helix (around 1300 cm -1 ) toward ordered β sheet (around 1230 cm -1 ) (see Fig.…”
Section: 49mentioning
confidence: 99%
“…It has been previously reported that the random component of the secondary structure plays a relevant role in the fibrillation process, as in the first stage of the fibrillation the native structure falls in a disordered structure and then develops into an organized β inter-chain structure. 62 It is important to recall that the peak at around 1670 cm -1 is the marker of ordered β sheet structures in the aggregated sample, and that this feature together with the narrowing of the amide I band are the most clear markers of fibril formation in the Raman spectra. 63 Shifts at lower frequencies can be observed in the amide III region coherently with the above mentioned changes in the secondary structure from α helix (around 1300 cm -1 ) toward ordered β sheet (around 1230 cm -1 ) (see Fig.…”
Section: 49mentioning
confidence: 99%
“…Advanced statistical methods allow for retrieving of reliable information from data sets that is not otherwise evident. We have been successful in developing and applying advanced statistical analysis of Raman spectra for biochemical [19][20][21][22][23][24][25][26][27] and forensic purposes [7][8][9]11,13].…”
Section: Open Accessmentioning
confidence: 99%
“…spectroscopy. We plan to apply advanced statistical methods, chemometrics in particular [88,89], to extract structural information from the spectroscopic data.…”
Section: Quantification Of Secondary Structural Transitionmentioning
confidence: 99%
“…This indicated that the partial unfolding of lysozyme is an all-or-none transition, i.e. two-state transition [88].…”
mentioning
confidence: 99%