The First Saudi Study Investigating the Plasmid-borne Aminoglycoside and Sulfonamide Resistance among Acinetobacter baumannii Clinical Isolates Genotyped by RAPD-PCR: the Declaration of a Novel Allelic Variant Called aac(6ʹ)-SL and Three Novel Mutations in the sul1 Gene in the Acinetobacter Plasmid (s)

El-Badawy, Mohamed F.; Abou-Elazm, Fatma I; Omar, Mohamed S.; El-Naggar, Mostafa E.; Maghrabi, Ibrahim A.

doi:10.2147/idr.s324707

Cited by 5 publications

(7 citation statements)

References 55 publications

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“…Ser 36 to Cys mutation will result in clashes with multiple residues from TMH2, TMH1, or TMH3, and abrogation of polar contacts with Ala 32 and Ser 33 . While the Ile 40 Cys mutation does not produce obvious clashes, its discriminative effect may be explained by its location within Ser 33 -Ala 41 TolQ region interacting with TolA.…”

Section: Atomic Resolution Cryo-em Spr Enabled Detailed Analysis Of I...mentioning

confidence: 91%

“…A. baumannii is one of the most dreaded and clinically important Gramnegative ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) pathogens causing a broad-spectrum nosocomial infection mainly among patients admitted to intensive care units (41)(42)(43)(44).…”

Section: Main Text Introductionmentioning

confidence: 99%

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Structural architecture of TolQ-TolR inner membrane protein complex from opportunistic pathogenAcinetobacter baumannii

Karimullina,

Guo,

Khan

et al. 2024

Preprint

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Gram-negative bacteria harness the proton motive force (PMF) within their inner membrane (IM) to uphold the integrity of their cell envelope, an indispensable aspect for both division and survival. The IM TolQ-TolR complex is the essential part of the Tol-Pal system, serving as a conduit for PMF energy transfer to the outer membrane. Here we present cryo-EM reconstructions ofAcinetobacter baumanniiTolQ in apo and TolR- bound forms at atomic resolution. The apo TolQ configuration manifests as a symmetric pentameric pore, featuring a trans-membrane funnel leading towards a cytoplasmic chamber. In contrast, the TolQ-TolR complex assumes a proton non-permeable stance, characterized by the TolQ pentamer’s flexure to accommodate the TolR dimer, where two protomers undergo a translation-based relationship. Our structure-guided analysis and simulations support the rotor-stator mechanism of action, wherein the rotation of the TolQ pentamer harmonizes with the TolR protomers’ interplay. These findings broaden our mechanistic comprehension of molecular stator units empowering critical functions within the Gram-negative bacterial cell envelope.TeaserApo TolQ and TolQ-TolR structures depict structural rearrangements required for cell envelope organization in bacterial cell division.

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Section: Atomic Resolution Cryo-em Spr Enabled Detailed Analysis Of I...mentioning

confidence: 91%

Section: Main Text Introductionmentioning

confidence: 99%

Structural architecture of TolQ-TolR inner membrane protein complex from opportunistic pathogenAcinetobacter baumannii

Karimullina,

Guo,

Khan

et al. 2024

Preprint

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“…Additionally, this study detected a new mutation in the sul1 gene and a novel allele variation in the aac (6’)-Ib gene. 63 Random amplified polymorphic-PCR-based genotyping of 23 MDR A. baumannii strains were executed, clustering them into 11 different genotypes, which revealed unseen community-acquired infections (CAI) in Egypt. 64 This also marked the first report regarding CAI in Egypt caused by A. baumannii .…”

Section: Random Amplified Polymorphic Dna-prcmentioning

confidence: 99%

Molecular epidemiology and molecular typing methods ofAcinetobacter baumannii

Anwer

2024

SMJ

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“…nosocomialis , and A . calcoaceticus account for about 80% of reported infections; making Acinetobacter baumannii complex (ABC) one of the most clinically important of HA pathogens [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

“…While many species of Acinetobacter can cause human disease, A. baumannii, A. pittii, A. nosocomialis, and A. calcoaceticus account for about 80% of reported infections; making Acinetobacter baumannii complex (ABC) one of the most clinically important of HA pathogens [1].…”

Section: Introductionmentioning

confidence: 99%

Acinetobacter baumannii complex, national laboratory-based surveillance in South Africa, 2017 to 2019

et al. 2022

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Objective We aimed to provide an analysis of A. baumannii complex (ABC) isolated from blood cultures in South Africa. Materials and methods ABC surveillance was conducted from 1 April 2017 to 30 September 2019 at 19 hospital sites from blood cultures of any age and sex. Organism identification was performed using the MALDI-TOF MS and antimicrobial susceptibility testing (AST), MicroScan Walkaway System. We confirmed colistin resistance with Sensititre, FRCOL panel, and selected for whole-genome sequencing. Results During the study period, we identified 4822 cases of ABC, of which 2152 cases were from 19 enhanced surveillance sites were reported during the enhanced surveillance period (1 August 2018 to 30 September 2019). Males accounted for 54% (2611/4822). Of the cases with known age, 41% (1968/4822) were infants (< 1-year-old). Seventy-eight percent (1688/2152) of cases had a known hospital outcome, of which 36% (602/1688) died. HIV status was known for 69% (1168/1688) of cases, and 14% (238/1688) were positive. Eighty-two percent (1389/1688) received antimicrobial treatment in admission. Three percent (35/1389) of cases received single colistin. Four percent (75/2033) were resistant to colistin. At least 75% of the isolates (1530/2033) can be classified as extensively drug-resistant (XDR), with resistance to most antibiotics except for colistin. The majority, 83% (20/24), of the colistin-resistant isolates were of the sequence type (ST) 1. Resistance genes, both plasmid- and chromosomal- mediated were not observed. Although all isolates had, nine efflux pump genes related to antimicrobial resistance. Conclusion Our surveillance data contributed to a better understanding of the natural course of A. baumannii disease, the patient characteristics among infants, and the level of resistance. At least two-thirds of the isolates were extensively drug-resistant, and four percent of isolates were resistant to colistin.

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Cited by 5 publications

References 55 publications

Structural architecture of TolQ-TolR inner membrane protein complex from opportunistic pathogenAcinetobacter baumannii

Structural architecture of TolQ-TolR inner membrane protein complex from opportunistic pathogenAcinetobacter baumannii

Molecular epidemiology and molecular typing methods ofAcinetobacter baumannii

Acinetobacter baumannii complex, national laboratory-based surveillance in South Africa, 2017 to 2019

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