The first report of Setaria tundra (Issaitshikoff &amp; Rajewskaya, 1928) in Slovakia by using of molecular methods

Čurlík, Ján; Šmigová, Júlia; Šmiga, Ľubomír; Lazár, Jozef; Lazár, Peter; Konjević, Dean; Papajová, I.

doi:10.1007/s11259-023-10127-9

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Setaria tundra was first described in Russia in 1928 [66], and, up to now, it has been reported in many European countries [67]. The reports from European countries are from (listed chronologically) Russia in 1928 [66], Austria in 1969 [68], Finland in 1970 [69], Sweden in 1973 [70], Norway in 1973 [71], Bulgaria in 1973 [72], Switzerland in 1974 [73], Germany in 1975 [74], Italy in 2003 [75], France in 2006 [76], Denmark in 2011 [77], Poland in 2010 [78], Hungary in 2013 [27], Spain in 2016 [79], Croatia in 2018 [24], and Slovakia in 2022 [25].…”

Section: Discussionmentioning

confidence: 99%

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Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia

Šiljegović,

Mouillaud,

Jiolle

et al. 2024

Animals

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Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health and particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite the extensive knowledge on these parasites, little is known about their natural vectors in Serbia. The parasite Setaria tundra, known to infect deer, has not yet been detected in Serbia but has been documented in neighboring countries. Thus, the aim of this study was to (i) further map out Dirofilaria sp. hotspots in the Vojvodina Province and detect S. tundra for the first time, (ii) detect positive mosquito species that can provide insights into how the nematodes spread in Serbia, and (iii) analyze the blood-fed female mosquitoes of species found to be infected, in order to identify the potential source of parasite infection. A total of 2902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood-fed (2521 specimens) and blood-fed (381 specimens) female mosquitos, in order to detect filarial nematode presence and identify blood-meal sources, respectively. When the parasite genome was detected, the amplicon (cox1 gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in Zrenjanin (August 2021) and Glogonj and Svetozar Miletić (both in July 2021). Additionally, Setaria tundra was detected in Aedes vexans collected in Iđoš (mid-August 2021) and Aedes caspius, which was collected in Mali Iđoš (end of July 2021). This work identifies two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra in Serbian territory. Blood-meal analysis provided insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.

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Section: Discussionmentioning

confidence: 99%

“…Setaria tundra (Issaitshikoff & Rajewskaya, 1928) has recently attracted interest in new studies [23][24][25] due to its expanding geographical range towards southern Europe and its negative impact on wild and semi-domesticated reindeer. Studies that performed the xenomonitoring of mosquitoes for the presence of Dirofilaria spp.…”

Section: Introductionmentioning

confidence: 99%

Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia

Šiljegović,

Mouillaud,

Jiolle

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

“…Setaria tundra was first described in Russia in 1928 [57] and up to now it has been reported in many European countries [58]. The reports from the European countries are given chronologically: Russia 1928 [57], Austria 1969 [59], Finland 1970 [60], Sweden 1973 [61], Norway 1973 [62], Bulgaria 1973 [63], Switzerland 1974 [64], Germany 1975 [65], Italy 2003 [66], France 2006 [67], Denmark 2011 [68], Poland 2010 [69], Hungary 2013 [70], Spain 2016 [71], Croatia 2018 [72], Slovakia 2022 [73].…”

Section: Discussionmentioning

confidence: 99%

<em>Dirofilaria</em> sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of<em> Setaria tundra</em> (Issaitshikoff &Amp; Rajewskaya, 1928) in Serbia

Šiljegović,

Mouillaud,

Jiolle

et al. 2024

Preprint

View full text Add to dashboard Cite

Dirofilaria immitis and D. repens are the two most widespread and important species of mosquito-borne nematodes, posing a significant threat to veterinary health, particularly affecting canines and felines. While D. immitis causes cardiopulmonary dirofilariasis, D. repens causes subcutaneous infections in dogs and other carnivores. Despite extensive knowledge about the aforementioned parasites, little is known about their natural vectors in Serbia. Thus, the aim of this study was to: i) further map out Dirofilaria sp. hotspots in the Vojvodina Province, ii) detect positive mosquito species which can provide insights in how the nematodes spread and adapt to the environmental conditions, and iii) analyze the blood fed mosquitoes females of species found infected to identify the potential source of parasite infection. A total of 2,902 female mosquitoes were collected across 73 locations during 2021 and 2022. Molecular biology methods, based on conventional PCR, were used to analyze non-blood fed (2,521 specimens) and blood fed (381 specimens) mosquito females, in order to detect filarial nematode presence and identify blood meal sources respectively. When the parasite genome was detected, the amplicon (COI gene, 650 bp fragment) was sent for Sanger sequencing, further confirming the presence of nematodes and species assignation. D. immitis was detected in three Culex pipiens mosquitoes collected in XXXX (location and date), while Setaria tundra was detected in Aedes vexans and Aedes caspius. This work adds two new locations where D. immitis occurs in Vojvodina, and is the first report of S. tundra on the territory of Serbia. Blood meal analysis provided insights into the preferences of mosquitoes that were positive for Dirofilaria sp. and S. tundra.

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Molecular detection of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) and Setaria cervi (Rudolphi, 1819) in red deer in Slovakia

Lazár,

Šmigová,

Šmiga

et al. 2024

Vet Res Commun

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Filaroid nematodes Setaria tundra (Issaitshikoff & Rajewskaya, 1928) and Setaria cervi (Rudolphi, 1819) are internal parasites from family Onchocercidae with occurrence in the northern hemisphere. They have a considerably wide range of final host, including many species of family Cervidae. Intermediate hosts and vectors at the same time, are represented by the several mosquito species, mostly of genus Aedes. Infection of Setaria is relatively harmless and especially in wild cervids usually pass unnoticed. Although in some cases it can induce peritonitis which might be a life threatening condition.This study was determined to reveal the presence of helminths Setaria tundra and Setaria cervi in red deer (Cervus elaphus) in Slovakia. The parasites were identified morphologically and genetically, based on the sequences of a fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. For this purpose we used partial results of our longer parasitological monitoring realized in one particular hunting area located in eastern Slovakia, near the city of Košice. A total of 60 red deer individuals were tested, of which one was found to be infected with Setaria tundra (prevalence of 1.7%) and four were detected to be infected with Setaria cervi (prevalence 6.7%). The intensity of infection was very low, only one specimen of Setaria spp. in each positive animal.

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The first report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Slovakia by using of molecular methods

Cited by 3 publications

References 18 publications

Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia

Dirofilaria sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) in Serbia

<em>Dirofilaria</em> sp. and Blood Meal Analysis in Mosquitoes Collected in Vojvodina and Mačva, and the First Report of<em> Setaria tundra</em> (Issaitshikoff &Amp; Rajewskaya, 1928) in Serbia

Molecular detection of Setaria tundra (Issaitshikoff & Rajewskaya, 1928) and Setaria cervi (Rudolphi, 1819) in red deer in Slovakia

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