The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein

Vargas-Bermúdez, Diana S.; Rendón-Marín, Santiago; Ruíz-Saenz, Julián; Mogollón, Darío; Correa, Jairo Jaime

doi:10.1371/journal.pone.0258311

Cited by 6 publications

(4 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PPV1 is one of the major causes of reproductive failure in swine worldwide, and the economic loss was high in the 1960s (Streck and Truyen 2020 ). For the particular clinical symptoms and affected tissues/organs of PPV2 to PPV7 are not determined, the pathogenesis of novel PPVs is unclear (Vargas-Bermudez et al 2021 ). Many researches have shown that PPV1 induced host cell apoptosis causing cell death, leading to sow reproductive failure (Zhang et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

TLR2-mediated NF-κB signaling pathway is involved in PPV1-induced apoptosis in PK-15 cells

Jin

Zhang

et al. 2022

Vet Res Commun

View full text Add to dashboard Cite

Porcine parvovirus 1 (PPV1) mainly induces severe reproductive failure in pregnant swine, and causes huge economic losses to the swine industry. Cell apoptosis induced by PPV1 infection has been identified the major cause of reproductive failure. However, the molecular mechanism was not fully elucidated. In this study, the potential mechanism of PPV1 induced apoptosis in PK-15 cells was investigated. Our results showed that PPV1 induced apoptosis in PK-15 cells. Further studies revealed toll-like receptor 2 (TLR2) was involved in the PPV1-mediated apoptosis. TLR2 siRNA significantly decreased the apoptosis. Finally, our study showed NF-κB was activated by TLR2 during PPV1-induced apoptosis. The activation of NF-κB signaling was demonstrated by the phosphorylation of p65, p65 nuclear translocation and degradation of inhibitor of kappa B α (IκBα). Together, these results provided evidence that the recognition between PPV1 and PK-15 cells was mainly through TLR2, and then induction of the NF-κB signaling pathway activation, which further induces apoptosis. Our study could provide information to understand the molecular mechanisms of PPV1 infection.

show abstract

Section: Discussionmentioning

confidence: 99%

TLR2-mediated NF-κB signaling pathway is involved in PPV1-induced apoptosis in PK-15 cells

Jin

Zhang

et al. 2022

Vet Res Commun

View full text Add to dashboard Cite

show abstract

“…The PPV7 strains have been reported to be divided into two different genotypes based on the complete genome sequences of PPV7 with MCC tree construction, while NJ and ML trees based on NS1 and Cap didn't display similar clusters ( 24 ). Another phylogenetic analysis with ML tree based on the capsid nucleotide sequences of PPV7 strains were made by Diana et al ( 14 ). Results showed that 52 PPV7 strains were divided into two different genotypes based on the presence or absence of a 5-aa deletion in the capsid protein.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, the most recently reported PPV7 was initially identified from healthy pigs' rectal swab samples using metagenomic sequencing in America in 2016. Subsequently, PPV7 was detected in Sweden ( 9 ), Poland ( 10 ), Korea ( 11 ), China ( 12 ), Brazil ( 13 ), and Colombian ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Characterization of Porcine Parvovirus 7 in Eastern Inner Mongolia Autonomous Region, China

Wen

Song

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Porcine parvoviruses (PPV) and porcine circoviruses type 2 (PCV2) are widespread in the pig population. Recently, it was suggested that PPV7 may stimulate PCV2 and PCV3 replication. The present study aimed to make detection and molecular characterization of PPV7 for the first time in eastern Inner Mongolia Autonomous Region, China. Twenty-seven of ninety-four samples (28.72%) and five in eight pig farms were PPV7 positive. Further detection showed that the co-infection rate of PPV7 and PCV2 was 20.21% (19/94), and 9.59% (9/94) for PPV7 and PCV3. In addition, the positive rate of PPV7 in PCV2 positive samples was higher than that in PCV2 negative samples, supporting that PCV2 could act as a co-factor for PPV7 infection. In total, four PPV7 strains were sequenced and designated as NM-14, NM-19, NM-4, and NM-40. The amplified genome sequence of NM-14 and NM-40 were 3,999nt in length, while NM-19 and NM-4 were 3,996nt with a three nucleotides deletion at 3,097–3,099, resulting in an amino acid deletion in the Cap protein. Phylogenetic analysis based on the capsid amino acid (aa) sequences showed that 52 PPV7 strains were divided into two clades, and the four PPV7 strains in this study were all clustered in clade 1. The genome and capsid amino acid sequence of the four PPV7 strains identified in this study shared 80.0–96.9% and 85.9–100% similarity with that of 48 PPV7 reference strains selected in NCBI. Simplot analysis revealed that NM-19 and NM-4 strains were probably produced by recombination of two PPV7 strains from China. The amino acid sequence alignment analysis of capsid revealed that the four PPV7 strains detected in Inner Mongolia had multiple amino acid mutations in the 6 B cell linear epitopes compared with the reference strains, suggesting that the four PPV7 strains may have different characteristics in receptor binding and immunogenicity. In summary, this paper reported the PPV7 infection and molecular characterization in the eastern of Inner Mongolia Autonomous Region for the first time, which is helpful to understand the molecular epidemic characteristics of PPV7.

show abstract

“…While there are differing views of PPV7 phylogenetic analysis, the closest approximation is from a study by Wang et al ( 28 ), in which 45 PPV7 sequences reported to GenBank (some complete and others partial) were analyzed, and two well-differentiated clades were identified ( 28 ). Diana et al divided PPV7 into two clades based on the insertion of five amino acids at the 180–184aa of the VP2 protein ( 29 ). In addition, phylogenetic analysis of the NS1 gene divided PPV7 into six clades, phylogenetic analysis of the cap gene divided PPV7 into four clades, and phylogenetic analysis of the complete genome divided PPV7 into six clades.…”

Section: Discussionmentioning

confidence: 99%

Genetic and epidemic characteristics of porcine parvovirus 7 in the Fujian and Guangdong regions of southern China

Zhang

Zheng

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Porcine parvovirus (PPV) is the primary cause of reproductive disorders in pigs. The porcine parvovirus 7 (PPV7) subtype was first identified in the United States in 2016. In this study, PPV7 was detected in different porcine samples, including serum, feces, saliva, and milk, from 69 pig farms in the Fujian and Guangdong regions of South China, and its coinfection with porcine circovirus 2 (PCV2), porcine circovirus 3 (PCV3), and porcine reproductive and respiratory syndrome virus (PRRSV) was determined. Whole-genome sequencing, phylogenetic analysis, and recombination analysis were performed on seven isolates, with each selected isolate originating from a different farm. There was a high rate of PPV7 positivity in blood, stool, and saliva but PPV7 DNA was absent from breast milk. The findings also showed that PPV7-positive samples had a high rate of coinfection with PCV2, PCV3, and PRRSV. Real-time PCR was used to determine the viral copy numbers of PCV2, PCV3, PRRSV, and PPV7 in serum samples and to assess whether PPV7 affected PCV2, PCV3, and PRRSV viral loads. Phylogenetic analysis showed that PPV7e and PPV7f were the most prevalent and widespread subtypes in the Fujian and Guangdong regions, respectively. While the PPV7a, PPV7b, PPV7c, and PPV7f subtypes were most prevalent in Fujian Province, PPV7a-e subtypes were prevalent in Guangdong, indicating that PPV7 has rich genetic diversity in these regions. A putative recombinant strain, 21FJ09, was identified using SimPlot and the Recombination Detection Program 4 software.

show abstract

The first report of porcine parvovirus 7 (PPV7) in Colombia demonstrates the presence of variants associated with modifications at the level of the VP2-capsid protein

Cited by 6 publications

References 57 publications

TLR2-mediated NF-κB signaling pathway is involved in PPV1-induced apoptosis in PK-15 cells

TLR2-mediated NF-κB signaling pathway is involved in PPV1-induced apoptosis in PK-15 cells

Detection and Molecular Characterization of Porcine Parvovirus 7 in Eastern Inner Mongolia Autonomous Region, China

Genetic and epidemic characteristics of porcine parvovirus 7 in the Fujian and Guangdong regions of southern China

Contact Info

Product

Resources

About