The First Report for the Presence of Spiroplasma and Rickettsia in Red Palm Weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in Egypt

Awad, Mona; Sharaf, A.; Elrahman, Tahany Abd; El-Saadany, Hassan Mohamed; ElKraly, Omnia Abdullah; Elnagdy, Sherif M.

doi:10.1007/s11686-020-00310-2

Cited by 3 publications

(3 citation statements)

References 64 publications

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“…The PCR cycle consisted of an initial denaturation step at 94 °C for 1 min, 35 cycles of denaturation at 94 °C for 30 s, annealing at 58 °C for 45 s, and extension at 72 °C for 1 min, followed by a final extension step at 72 °C for 10 min. PCR products were visualized by running on a 2% TAE-agarose gel stained with ethidium bromide [ 7 , 79 ].…”

Section: Methodsmentioning

confidence: 99%

Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation

ElKraly,

Awad,

El-Saadany

et al. 2023

anim microbiome

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Endosymbionts are known to have significant effects on their insect hosts, including nutrition, reproduction, and immunity. Insects gut microbiota is a critical component that affects their physiological and behavioral characteristics. The black cutworm (BCW), Agrotis ipsilon, is an economically important lepidopteran pest that has a diverse gut microbiome composed of nine species belonging to three phyla: Proteobacteria, Actinobacteria, and Firmicutes. This study was conducted to investigate the diversity of gut bacteria isolated from BCW larvae and moths and their effects on metabolism and pesticide degradation. The bacterial isolates were identified using the 16 S rRNA gene. The study showed that the gut microbiome composition significantly affected the metabolism of BCW larvae. Based on the screening results of synthesis of digestive enzymes and pesticide degradation, Brachybacterium conglomeratum and Glutamicibacter sp were selected to perform the remaining experiments as single isolates and consortium. The consortium-fed larvae showed high metabolic indices compared to antibiotic-fed larvae and the control. The gut bacteria were also shown to degrade three pesticide groups. Concerns regarding the health risk of chlorpyrifos have been raised due to its extensive use in agriculture. The isolated B. conglomeratum was more effective in chlorpyrifos degradation than the consortium. Furthermore, the study also examined the presence of sex related endosymbionts (Wolbachia, Spiroplasma, and Rickettsia) in the reproductive tissues of adults. The outcomes demonstrated that none of the examined endosymbionts existed. In conclusion, the study highlights the importance of the gut microbiome in insect physiology and behavior and its potential applications in biotechnology. It provides insights into developing eco-friendly pest control and bioremediation strategies using gut bacteria.

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Section: Methodsmentioning

confidence: 99%

Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation

ElKraly,

Awad,

El-Saadany

et al. 2023

anim microbiome

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“…Palm weevils host a diverse assemblage of protozoan, fungal, viral, and bacterial species, the majority of which have been documented in R. ferrugineus (18,29,113,145,152,191). A γ-proteobacterial endosymbiont lineage, Nardonella, an intracellular obligate specialist, is found in symbiotic organs called bacteriomes in R. ferrugineus.…”

Section: Symbionts Microbiome and Endosymbiontsmentioning

confidence: 99%

“…Interestingly, Wolbachia, a sexratio-distorting rickettsia-like organism commonly associated with weevils (147), has not been isolated from Rhynchophorus spp. (15,29). Gut microbiome studies of larval R. ferrugineus are dominated by metagenomes of proteobacteria.…”

Section: Symbionts Microbiome and Endosymbiontsmentioning

confidence: 99%

Taxonomy, Biology, Symbionts, Omics, and Management of Rhynchophorus Palm Weevils (Coleoptera: Curculionidae: Dryophthorinae)

Hoddle,

Antony,

El-Shafie

et al. 2024

Annu. Rev. Entomol.

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Palm weevils, Rhynchophorus spp., are destructive pests of native, ornamental, and agricultural palm species. Of the 10 recognized species, two of the most injurious species, Rhynchophorus ferrugineus and Rhynchophorus palmarum, both of which have spread beyond their native range, are the best studied. Due to its greater global spread and damage to edible date industries in the Middle East, R. ferrugineus has received more research interest. Integrated pest management programs utilize traps baited with aggregation pheromone, removal of infested palms, and insecticides. However, weevil control is costly, development of resistance to insecticides is problematic, and program efficacy can be impaired because early detection of infestations is difficult. The genome of R. ferrugineus has been sequenced, and omics research is providing insight into pheromone communication and changes in volatile and metabolism profiles of weevil-infested palms. We outline how such developments could lead to new control strategies and early detection tools.

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Exploring Gut Microbiota in Red Palm Weevil (Rhynchophorus ferrugineus): Effects on Pest Management, Pesticide Resistance, and Thermal Stress Tolerance

Elkraly,

Elrahman,

Awad

et al. 2024

Microbiology Research

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The red palm weevil (RPW), Rhynchophorus ferrugineus, poses a significant threat to date palms globally, heavily relying on symbiotic microbes for various physiological and behavioral functions. This comprehensive study delves into the intricate dynamics of RPW gut microbiota, revealing a diverse microbial community consisting of seven genera and eight species from Proteobacteria, Firmicutes, and Actinobacteria. The stability of gut bacteria across different life stages was observed, with notable impacts on larval metabolism attributed to shifts in bacterial composition. Bacillus subtilis emerged as a key player, producing a spectrum of metabolic enzymes. Furthermore, the gut bacteria exhibited remarkable pesticide degradation capabilities, suggesting a potential role in the host’s resistance to pesticides. The Arthrobacter sp. was identified as a promising candidate for eco-friendly pest biocontrol and biodegradation strategies. Investigating the influence of thermal stress on two groups of RPW larvae (conventional-fed and antibiotic-fed) at varying temperatures (15, 27, and 35 °C) unveiled potential survival implications. This study highlights the pivotal role of bacterial symbionts in enabling larvae adaptation and thermal stress tolerance. In essence, this research contributes crucial insights into the diversity and functions of RPW gut bacteria, emphasizing their prospective applications in pest control strategies.

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The First Report for the Presence of Spiroplasma and Rickettsia in Red Palm Weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in Egypt

Cited by 3 publications

References 64 publications

Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation

Impact of gut microbiota composition on black cutworm, Agrotis ipsilon (hufnagel) metabolic indices and pesticide degradation

Taxonomy, Biology, Symbionts, Omics, and Management of Rhynchophorus Palm Weevils (Coleoptera: Curculionidae: Dryophthorinae)

Exploring Gut Microbiota in Red Palm Weevil (Rhynchophorus ferrugineus): Effects on Pest Management, Pesticide Resistance, and Thermal Stress Tolerance

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