The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3′ processing

Abe, Ken‐ichiro; Yamamoto, Ryusuke; Franke, Vedran; Cao, Minjun; Suzuki, Yutaka; Suzuki, Masataka; Vlahoviček, Kristian; Svoboda, Petr; Schultz, Richard M.; Aoki, Fugaku

doi:10.15252/embj.201490648

Cited by 135 publications

(168 citation statements)

References 62 publications

(93 reference statements)

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“…Such patterns also emerge in independent NGS data (Supplemental Fig. S3A; Abe et al 2015) and are consistent with the literature (Kigami et al 2003;Peaston et al 2004;Svoboda et al 2004;Flemr et al 2013). A survey of individual co-opted ERVL LTR promoters reveals two main expression patterns during OET ( Fig.…”

Section: Ervl Ltr-mediated Control Of Gene Expression During Oetsupporting

confidence: 89%

“…We discovered that the MTA LTR can function as an alternative promoter and produce a second Dicer1 O -like transcript. Typical expression from the MTA LTR is minimal (<10% considering NGS data) (Smallwood et al 2011;Abe et al 2015;Veselovska et al 2015;Karlic et al 2017), possibly because the upstream MTC LTR promoter reduces transcription from the MTA LTR. Upon the MTC LTR deletion, the MTA LTR promoter activity in the mixed genetic background increases (Fig.…”

Section: Variability and Plasticity Of Mt Ltr Promoter Activity In Comentioning

confidence: 99%

“…Mouse ZGA offers a unique model for examining such transcription because the maternal transcriptome has a reduced intronic and intergenic NGS signal (Abe et al 2015), thereby facilitating detection of nascent transcripts in the zygote. Furthermore, promoter activity of several LTRs, including MT2 and ORR1A, appears during ZGA (Fig.…”

Section: Scanning Of Genetic Information Downstream From Ervl Ltrsmentioning

confidence: 99%

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Long terminal repeats power evolution of genes and gene expression programs in mammalian oocytes and zygotes

et al. 2017

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Retrotransposons are "copy-and-paste" insertional mutagens that substantially contribute to mammalian genome content. Retrotransposons often carry long terminal repeats (LTRs) for retrovirus-like reverse transcription and integration into the genome. We report an extraordinary impact of a group of LTRs from the mammalian endogenous retrovirus-related ERVL retrotransposon class on gene expression in the germline and beyond. In mouse, we identified more than 800 LTRs from ORR1, MT, MT2, and MLT families, which resemble mobile gene-remodeling platforms that supply promoters and first exons. The LTR-mediated gene remodeling also extends to hamster, human, and bovine oocytes. The LTRs function in a stagespecific manner during the oocyte-to-embryo transition by activating transcription, altering protein-coding sequences, producing noncoding RNAs, and even supporting evolution of new protein-coding genes. These functions result, for example, in recycling processed pseudogenes into mRNAs or lncRNAs with regulatory roles. The functional potential of the studied LTRs is even higher, because we show that dormant LTR promoter activity can rescue loss of an essential upstream promoter. We also report a novel protein-coding gene evolution-D6Ertd527e-in which an MT LTR provided a promoter and the 5

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Section: Ervl Ltr-mediated Control Of Gene Expression During Oetsupporting

confidence: 89%

Section: Variability and Plasticity Of Mt Ltr Promoter Activity In Comentioning

confidence: 99%

Section: Scanning Of Genetic Information Downstream From Ervl Ltrsmentioning

confidence: 99%

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Long terminal repeats power evolution of genes and gene expression programs in mammalian oocytes and zygotes

et al. 2017

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“…However, splicing is not inefficient in the mouse zygote (Abe et al, 2015). To determine whether BCAS2 was involved in DNA repair in mouse zygotes, the correlation between BCAS2 and γH2AX, a marker of DNA damage, was assessed in zygotes of relevant pronuclear stages (PN) by staining.…”

Section: Bcas2 Responds To Dna Damage In Mouse Zygotesmentioning

confidence: 99%

Maternal BCAS2 protects genomic integrity in mouse early embryonic development

Wang

Xiang

et al. 2015

Development

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Mammalian early embryos maintain accurate genome integrity for proper development within a programmed timeline despite constant assaults on their DNA by replication, DNA demethylation and genetic defects transmitted from germ cells. However, how genome integrity is safeguarded during mammalian early embryonic development remains unclear. BCAS2 (breast carcinoma amplified sequence 2), a core component of the PRP19 complex involved in pre-mRNA splicing, plays an important role in the DNA damage response through the RPA complex, a key regulator in the maintenance of genome integrity. Currently, the physiological role of BCAS2 in mammals is unknown. We now report that BCAS2 responds to endogenous and exogenous DNA damage in mouse zygotes. Maternal depletion of BCAS2 compromises the DNA damage response in early embryos, leading to developmental arrest at the two-to four-cell stage accompanied by the accumulation of damaged DNA and micronuclei. Furthermore, BCAS2 mutants that are unable to bind RPA1 fail in DNA repair during the zygotic stage. In addition, phosphorylated RPA2 cannot localise to the DNA damage sites in mouse zygotes with disrupted maternal BCAS2. These data suggest that BCAS2 might function through the RPA complex during DNA repair in zygotes. Together, our results reveal that maternal BCAS2 maintains the genome integrity of early embryos and is essential for female mouse fertility.

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“…The acquisition of H3K4me2/ me3 in the male pronucleus, pronuclear fusion and cell division precede zygotic genome activation (Aoshima et al, 2015), although in the mouse minor zygotic transcription activity has been detected at the pronuclear stage (Abe et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Setd1b, encoding a histone 3 lysine 4 methyltransferase, is a maternal effect gene required for the oogenic gene expression program

et al. 2017

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Germ cell development involves major reprogramming of the epigenome to prime the zygote for totipotency. Histone 3 lysine 4 (H3K4) methylations are universal epigenetic marks mediated in mammals by six H3K4 methyltransferases related to fly Trithorax, including two yeast Set1 orthologs: Setd1a and Setd1b. Whereas Setd1a plays no role in oogenesis, we report that Setd1b deficiency causes female sterility in mice. Oocyte-specific Gdf9-iCre conditional knockout (Setd1b Gdf9 cKO) ovaries develop through all stages; however, follicular loss accumulated with age and unfertilized metaphase II (MII) oocytes exhibited irregularities of the zona pellucida and meiotic spindle. Most Setd1b Gdf9 cKO zygotes remained in the pronuclear stage and displayed polyspermy in the perivitelline space. Expression profiling of Setd1bGdf9 cKO MII oocytes revealed (1) that Setd1b promotes the expression of the major oocyte transcription factors including Obox1, 2, 5, 7, Meis2 and Sall4; and (2) twice as many mRNAs were upregulated than downregulated, suggesting that Setd1b also promotes the expression of negative regulators of oocyte development with multiple Zfp-KRAB factors implicated. Together, these findings indicate that Setd1b serves as maternal effect gene through regulation of the oocyte gene expression program.

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The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3′ processing

Cited by 135 publications

References 62 publications

Long terminal repeats power evolution of genes and gene expression programs in mammalian oocytes and zygotes

Long terminal repeats power evolution of genes and gene expression programs in mammalian oocytes and zygotes

Maternal BCAS2 protects genomic integrity in mouse early embryonic development

Setd1b, encoding a histone 3 lysine 4 methyltransferase, is a maternal effect gene required for the oogenic gene expression program

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