The First Metallo-β-Lactamase Identified in Norway Is Associated with a TniC-Like Transposon in a
            <i>Pseudomonas aeruginosa</i>
            Isolate of Sequence Type 233 Imported from Ghana

Samuelsen, Ørjan; Buarø, Liselotte; Toleman, Mark A.; Giske, Christian G.; Hermansen, Nils Olav; Walsh, Timothy R.; Sundsfjord, Arnfinn

doi:10.1128/aac.00785-08

Cited by 27 publications

(19 citation statements)

References 10 publications

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“…Interestingly, the integron lacked the 3= conserved region (3=CS) of qac⌬E1-sul1 and harbored the tniC at the 3= end, which is characteristic of Tn5090/Tn402 (29). The sequenced part of the integron (13). Also, the integrons were similar, with only a few nucleotide differences, to an integron identified in India (GenBank accession no.…”

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confidence: 77%

“…VIM-2-producing P. aeruginosa isolates have been observed in Tunisia (6)(7)(8), Kenya (9), the Ivory Coast (10), Algeria (11), and South Africa (12). Further, reports have shown the import of P. aeruginosa with VIM-2 from African countries (Ghana, Tunisia, and Egypt) into Europe (13)(14)(15).…”

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confidence: 97%

“…This observation indicates both the chromosomal and plasmid locations of bla VIM-2 . In addition to the identification of the integron structure in ST244 and ST640, this genetic structure has been identified in several other different genetic backgrounds of P. aeruginosa, such as ST233 (13,31), ST234 (27), ST235 (27,33), and ST1488 (10), with wide geographical distribution. This suggests that the integron is associated with mobile genetic structures facilitating horizontal transfer and dissemination, as previously suggested (29).…”

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confidence: 99%

“…The presence of MBL genes was investigated by PCR, as described previously (16). The molecular typing of the MBL-producing isolates was per-formed by serotyping, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and sequencing of the integrons, as previously described (13,15). The genomic localization of bla VIM-2 was determined by I-CeuI PFGE, followed by Southern blotting and hybridization with nonradioactive labeled bla VIM-2 and 16S rRNA probes.…”

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Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla _VIM-2 in a TniC Integron

Moyo

Haldorsen

Aboud

et al. 2015

Antimicrob Agents Chemother

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e Epidemiological data on carbapenemase-producing Gram-negative bacteria on the African continent are limited. Here, we report the identification of VIM-2-producing Pseudomonas aeruginosa isolates in Tanzania. Eight out of 90 clinical isolates of P. aeruginosa from a tertiary care hospital in Dar es Salaam were shown to harbor bla VIM-2 . The bla VIM-2 -positive isolates belonged to two different sequence types (ST), ST244 and ST640, with bla VIM-2 located in an unusual integron structure lacking the 3= conserved region of qac⌬E1-sul1. Pseudomonas aeruginosa is an opportunistic pathogen associated with a number of nosocomial infections. Carbapenems (i.e., meropenem and imipenem) are often the treatment of choice for infections caused by P. aeruginosa that is resistant to other antipseudomonal ␤-lactams (1). However, the emergence and spread of acquired carbapenemases, particularly metallo-␤-lactamases (MBLs), among P. aeruginosa are threatening the usefulness of carbapenems (2). Resistance to carbapenems in P. aeruginosa can also occur due to impermeability, efflux mechanisms, and other ␤-lactamases, including overexpression of the chromosomal AmpC (3).Several acquired MBLs have been identified in P. aeruginosa, including the VIM, IMP, SPM, GIM, AIM, FIM, and NDM enzymes (2, 4). The genes encoding these MBLs are associated with mobile genetic elements, such as insertion sequence common region (ISCR) elements, transposons, and plasmids, and as gene cassettes in integron structures. The dissemination of the various MBLs among P. aeruginosa isolates has been shown to occur in many different genetic backgrounds (5). However, two major clonal complexes (CC), CC235 and CC111, predominate, particularly with respect to the dissemination of VIM enzymes (5).On the African continent, an increasing number of reports indicates that MBL-producing, and in particular VIM-2-producing, P. aeruginosa is widespread in Africa. VIM-2-producing P. aeruginosa isolates have been observed in Tunisia (6-8), Kenya (9), the Ivory Coast (10), Algeria (11), and South Africa (12). Further, reports have shown the import of P. aeruginosa with VIM-2 from African countries (Ghana, Tunisia, and Egypt) into Europe (13-15).(Part of this study was presented at the 22nd European Congress of Microbiology and Infectious Diseases, 31 March to 3 April 2012, London, United Kingdom.)The aim of this study was to determine the occurrence and molecular epidemiology of MBL-producing P. aeruginosa isolates identified from clinical specimens at a tertiary hospital in Dar es Salaam, Tanzania. This was a cross-sectional study conducted at the Central Pathology Laboratory, Muhimbili National Hospital (MNH), Dar es Salaam, Tanzania. MNH is the largest tertiary health care facility in Tanzania and serves as a university teaching and referral hospital to the population of Dar es Salaam and the whole country. The study included 90 clinical isolates of P. aeruginosa consecutively collected from May 2010 to July 2011. Duplicate isolates were excluded from the study. Only i...

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Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla _VIM-2 in a TniC Integron

Moyo

Haldorsen

Aboud

et al. 2015

Antimicrob Agents Chemother

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“…The product of the gene is a resolvase, TniC, which is involved in transposition and plays an important role in spreading MBL genes (21). It seems that the cooperative action of several transposon elements contributes to the dissemination of bla IMP gene among different species of Enterobacteriaceae.…”

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First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

Sun

Han

et al. 2011

Antimicrob Agents Chemother

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The study shows for the first time the presence of the Klebsiella oxytoca strain fp10 coproducing plasmidmediated KPC-2 and IMP-8 carbapenemases. The strain was obtained from the fecal sample of an inpatient and showed high-level resistance to imipenem and ertapenem (MICs > 32 g/ml). Conjugation experiments demonstrated the transferability of the carbapenem-resistant determinants. The results of plasmid analysis and Southern hybridization revealed that the bla KPC-2 gene was located on transferable plasmid pFP10-1 (ϳ54 kb), whereas the bla IMP-8 gene was on transferable plasmid pFP10-2 (ϳ180 kb). Analysis of the genetic environment of these two genes has demonstrated that ISKpn6 and ISKpn8 are involved in the spread of the bla KPC-2 gene, while the transposable elements IS26, intI1, and tniC might contribute to the dissemination of the bla IMP-8 gene. The chimera of several transposon-associated elements indicated a novel genetic environment of IMP-type metallo-␤-lactamase gene in Enterobacteriaceae from China.

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WITHDRAWN: Antibiotic resistance in Pseudomonas aeruginosa – mechanisms, epidemiology and evolution

Botelho

Grosso

Peixe

2019

Drug Resistance Updates

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The First Metallo-β-Lactamase Identified in Norway Is Associated with a TniC-Like Transposon in a Pseudomonas aeruginosa Isolate of Sequence Type 233 Imported from Ghana

Cited by 27 publications

References 10 publications

Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla _VIM-2 in a TniC Integron

Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla _VIM-2 in a TniC Integron

First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

WITHDRAWN: Antibiotic resistance in Pseudomonas aeruginosa – mechanisms, epidemiology and evolution

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The First Metallo-β-Lactamase Identified in Norway Is Associated with a TniC-Like Transposon in a Pseudomonas aeruginosa Isolate of Sequence Type 233 Imported from Ghana

Cited by 27 publications

References 10 publications

Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla VIM-2 in a TniC Integron

Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla VIM-2 in a TniC Integron

First Report of Klebsiella oxytoca Strain Coproducing KPC-2 and IMP-8 Carbapenemases

WITHDRAWN: Antibiotic resistance in Pseudomonas aeruginosa – mechanisms, epidemiology and evolution

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Product

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Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla _VIM-2 in a TniC Integron

Identification of VIM-2-Producing Pseudomonas aeruginosa from Tanzania Is Associated with Sequence Types 244 and 640 and the Location of bla _VIM-2 in a TniC Integron