The first metagenome of activated sludge from full-scale anaerobic/anoxic/oxic (A2O) nitrogen and phosphorus removal reactor using Illumina sequencing

Tian, Mei; Zhao, Fangqing; Shen, Xin; Chu, Ka Hou; Wang, Jinfeng; Chen, Shuai; Guo, Yan; Liu, Hanhu

doi:10.1016/j.jes.2014.12.027

Cited by 121 publications

(33 citation statements)

References 45 publications

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“…Scientific literature reports hundreds of applications of conventional and innovative biological tools, as well as examples of investigations based on ecological criteria [9,[20][21][22][23][24][25][26][27]. Almost all the biological processes have been explored in terms of biomass composition (bacteria, protozoa, metazoan, and fungi) and activity: conventional activated sludge, attached biomass, membrane reactors, and treating either municipal [27][28][29][30][31][32][33][34] or industrial wastewater [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Abbà

Collivignarelli

Manenti

et al. 2017

Journal of Chemistry

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A thermophilic aerobic membrane reactor (TAMR) treating high-strength COD liquid wastes was submitted to an integrated investigation, with the aim of characterizing the biomass and its rheological behaviour. These processes are still scarcely adopted, also because the knowledge of their biology as well as of the physical-chemical properties of the sludge needs to be improved. In this paper, samples of mixed liquor were taken from a TAMR and submitted to fluorescent in situ hybridization for the identification and quantification of main bacterial groups. Measurements were also targeted at flocs features, filamentous bacteria, and microfauna, in order to characterize the sludge. The studied rheological properties were selected as they influence significantly the performances of membrane bioreactors (MBR) and, in particular, of the TAMR systems that operate under thermophilic conditions (i.e., around 50 ∘ C) with high MLSS concentrations (up to 200 gTS L −1 ). The proper description of the rheological behaviour of sludge represents a useful and fundamental aspect that allows characterizing the hydrodynamics of sludge suspension devoted to the optimization of the related processes. Therefore, in this study, the effects on the sludge rheology produced by the biomass concentration, pH, temperature, and aeration were analysed.

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Section: Introductionmentioning

confidence: 99%

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Abbà

Collivignarelli

Manenti

et al. 2017

Journal of Chemistry

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“…Under anaerobic conditions, the present study has shown that the bacterial community of successful EBPR was predominantly containing phosphate-accumulating bacteria such as unclassified Rhodocyclaceae (47.64%) [8,38], followed by glycogen-accumulating organisms (GAOs) such as Propionivibrio (8.59%) [39]. Even though many EBPR wastewater treatment plants run well, the deterioration of their pollutant removal capacity due to the competition between beneficial (PAOs) and detrimental (GAOs) organisms have also been reported [5,33]. It was further observed that the anaerobic zone of the successful EBPR also had high abundance of an additional bacterial community responsible for nitrification and denitrification, such as unclassified Comamonadaceae (4.55), unclassified Burkholderiales incertae sedis (4.38), Planctomyces (1.68), Nitrospira (1.68), unclassified Nitrosomonadaceae (1.68), unclassified Anaerolineaceae (3.7), and so on [3,7,33].…”

Section: Bacterial Community Structure In Both Wastewater Treatment Pmentioning

confidence: 84%

“…This has been confirmed by Ye and Zhang [20], who reported Proteobacteria as the predominant bacterial phylum in several activated sludge samples. Furthermore, the predominance of Bacteriodetes over Proteobacteria in EBPR or activated sludge has not been intensively reported [32][33].…”

Section: Bacterial Community Structure In Both Wastewater Treatment Pmentioning

confidence: 99%

“…Even though many EBPR wastewater treatment plants run well, the deterioration of their pollutant removal capacity due to the competition between beneficial (PAOs) and detrimental (GAOs) organisms have also been reported [5,33]. It was further observed that the anaerobic zone of the successful EBPR also had high abundance of an additional bacterial community responsible for nitrification and denitrification, such as unclassified Comamonadaceae (4.55), unclassified Burkholderiales incertae sedis (4.38), Planctomyces (1.68), Nitrospira (1.68), unclassified Nitrosomonadaceae (1.68), unclassified Anaerolineaceae (3.7), and so on [3,7,33]. In contrast, despite the similarity Fig.…”

Section: Bacterial Community Structure In Both Wastewater Treatment Pmentioning

confidence: 99%

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Comparing Bacterial Diversity in Two Full-Scale Enhanced Biological Phosphate Removal Reactors Using 16S Amplicon Pyrosequencing

Kamika¹,

Azizi²,

Tekere³

2018

Pol. J. Environ. Stud.

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Despite their stability and the widespread use of enhanced biological phosphorus removal (EBPR), little is known about their microbial composition and activity. In our study we investigated highthroughput pyrosequencing of bacterial communities from two full-scale EBPR reactors of South Africa. Findings indicated that both EBPRs harboured high bacterial similarity, ranging from 83 to 100% with a diverse community dominated by Proteobacteria (57.04 to 79.48% for failed EBPR and 61.7 to 85.39% for successful EBPR) throughout the five selected treatment zones with the exception of the fermenter (Bacteroidetes: 55.84%) from the successful EBPR. However, a lower dissimilarity was observed with the presence of 70 unique bacterial genera from successful EBPRs belonging to Gammaproteobacteria, Betaproteobacteria, and Actinobacteria, while 69 unique genera from failed EBPR belonged to Alphaproteobacteria, Betaproteobacteria, and Clostridia. The failed EBPR (54.58%) revealed less fermenting bacteria in the fermenter as compared to the successful EBPR (73.58%). More detrimental organisms and less nitrifying/denitrifying bacteria were also found in failed EBPR than in the successful EBPR, as well as phosphate-accumulating bacteria. Canonical correspondence analysis (CCA) displayed a very low relationship between microbial patterns, pH and DO -suggesting that these environmental factors played a major role in community dissimilarity. Aerobic zones appeared to have the highest dissimilarity between both EBPRs, with the failed EBPR predominated by Acidovorax (26.2%) and the successful EBPR with unclassified Rhodocyclaceae (37.24%). Furthermore, 21.47% of readings (failed EBPR) and 17.18% of readings (successful EBPR) could not be assigned to taxonomic classifications, highlighting the high diversity level of novel microbial species in such an environment.

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“…Typical nitrogen removal microorganisms are dominant in Proteobacteria, Bacteroidetes, and Planctomycetes, and they are detected in different sewage treatment processes (Tian et al, ). Proteobacteria have been reported by many studies to contain common denitrifiers and play a major role in N‐cycle for the removal of nitrogen (Shu, He, Yue, & Wang, ; Tan et al, ).…”

Section: Resultsmentioning

confidence: 99%

A novel pilot‐scale tubular bioreactor‐enhanced floating treatment wetland for efficient in situ nitrogen removal from urban landscape water: Long‐term performance and microbial mechanisms

Cui

Yang

Ding

et al. 2019

Water Environment Research

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In order to strengthen in situ nitrogen removal of urban landscape water, a novel pilot-scale tubular bioreactor-enhanced floating treatment wetland (TB-EFTW) was constructed, and the long-term performance and responsible microbial mechanisms were investigated in this study. The results showed that the system could remove 81.5% nitrogen from the landscape water after 240 days' operation. Moreover, the contribution rate of plant absorption to nitrogen was low (8.3%), which indicated that microbial biotransformation rather than plant absorption played a more key role in nitrogen removal in TB-EFTW system. The declining dissolved oxygen (DO) concentration along the axial direction of tubular bioreactor (TB) resulted in the sequential bacterial community of nitrifying, aerobic denitrifying, and anoxic denitrifying bacteria in the front, middle, and final part of TB. High-throughput sequencing results demonstrated that the internal environment of the system realized the coexistence of nitrifying, aerobic denitrifying and anoxic denitrifying process. The reason was mainly because that oxic-anoxic (O-A) areas were formed in sequence along the axial direction of tubular bioreactor. Overall, a unique advantage in nitrogen removal was achieved in TB-EFTW, which could provide important references for in situ treatment of urban landscape water. • Practitioner points• TB-EFTW strengthened nitrogen removal for in situ urban landscape water treatment. • Microbial conversion played a key role in nitrogen removal of the TB-EFTW system. • The unique distribution of oxic-anoxic (O-A) areas was formed in sequence along the TB. • Nitrification, aerobic, and anoxic denitrification were synergistically involved in the TB.• Key words agriculture; biological treatment; nitrogen removal; surface water

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The first metagenome of activated sludge from full-scale anaerobic/anoxic/oxic (A2O) nitrogen and phosphorus removal reactor using Illumina sequencing

Cited by 121 publications

References 45 publications

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Rheology and Microbiology of Sludge from a Thermophilic Aerobic Membrane Reactor

Comparing Bacterial Diversity in Two Full-Scale Enhanced Biological Phosphate Removal Reactors Using 16S Amplicon Pyrosequencing

A novel pilot‐scale tubular bioreactor‐enhanced floating treatment wetland for efficient in situ nitrogen removal from urban landscape water: Long‐term performance and microbial mechanisms

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