The first evaluation relationship of integron genes and the multidrug-resistance in class A ESBLs genes in enteropathogenic Escherichia coli strains isolated from children with diarrhea in Southwestern Iran

Jomehzadeh, Nabi; Ahmadi, Khadijeh; Javaherizadeh, Hazhir; Afzali, Maryam

doi:10.1007/s11033-020-06047-5

Cited by 7 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typical EPEC strains (tEPEC) have the eae and bfp (gene encoding for bundle-forming pili) genes but no Shiga toxin genes ( stx ). Often known as atypical EPEC, certain clinical isolates of EPEC lack the bfp gene [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features

Nouri

Hasani

Shirazi

et al. 2021

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Accumulating evidence indicates that specific strains of mucosa-associated Escherichia coli (E. coli) can influence the development of colorectal carcinoma. This study aimed to investigate the prevalence and characterization of mucosa-associated E. coli obtained from the colorectal cancer (CRC) patients and control group. At two referral university-affiliated hospitals in northwest Iran, 100 patients, 50 with CRC and 50 without, were studied over the course of a year. Fresh biopsy specimens were used to identify mucosa-associated E. coli isolates after dithiothreitol mucolysis. To classify the E. coli strains, ten colonies per sample were typed using enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR). The strains were classified into phylogroups using the quadruplex PCR method. The PCR method was used to examine for the presence of cyclomodulin, bfp, stx1, stx2, and eae-encoding genes. The strains were tested for biofilm formation using the microtiter plate assay. CRC patients had more mucosa-associated E. coli than the control group ( p < 0.05 ). Enteropathogenic Escherichia coli (EPEC) was also found in 23% of CRC strains and 7.1% of control strains ( p < 0.05 ). Phylogroup A was predominant in control group specimens, while E. coli isolates from CRC patients belonged most frequently to phylogroups D and B2. Furthermore, the frequency of cyclomodulin-encoding genes in the CRC patients was significantly higher than the control group. Around 36.9% of E. coli strains from CRC samples were able to form biofilms, compared to 16.6% E. coli strains from the control group ( p < 0.05 ). Noticeably, cyclomodulin-positive strains were more likely to form biofilm in comparison to cyclomodulin-negative strains ( p < 0.05 ). In conclusion, mucosa-associated E. coli especially cyclomodulin-positive isolates from B2 and D phylogroups possessing biofilm-producing capacity colonize the gut mucosa of CRC patients.

show abstract

Section: Introductionmentioning

confidence: 99%

Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features

Nouri

Hasani

Shirazi

et al. 2021

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

show abstract

“…Type 1 fimbriae, encoded by the fimA gene, is associated with biofilm formation and commonly found in most of the E . coli isolates, conferring as an important virulence factor [ 18 ]. Similar to our study, Nojoomi et al [ 45 ] reported that fimA was the most frequent virulence gene in clinical E .…”

Section: Discussionmentioning

confidence: 99%

“…coli isolates were collected from clinical specimens (including urine, sputum, wound, and blood) of patients admitted to affiliated hospitals of the Abadan University of Medical Sciences. All isolates were accurately identified by performing standard methods [ 18 ]. Briefly, the specimens were inoculated in MacConkey broth (Merck, Darmstadt, Germany) and incubated at 37°C for 24 h. A loopful of broth culture was subsequently cultured on Eosin Methylene Blue (EMB; Biolife Italiana, Italy) and MacConkey agar.…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, the specimens were inoculated in MacConkey broth (Merck, Darmstadt, Germany) and incubated at 37°C for 24 h. A loopful of broth culture was subsequently cultured on Eosin Methylene Blue (EMB; Biolife Italiana, Italy) and MacConkey agar. All grown lactose-fermenting colonies were identified via bacteriological tests (such as hemolytic activity on blood agar, motility test, and Gram staining result) and conventional biochemical tests including triple sugar iron agar, oxidase, catalase, production of lysine decarboxylase, citrate utilization test, Sulfur Indole Motility (SIM), Methyl Red & Vogues-Proskauer (MR-VP), and urease test [ 18 , 19 ]. Finally, purified isolates stored in trypticase soy broth (Merck, Darmstadt, Germany) containing 20% glycerol at -70°C until further use.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Virulence-associated genes analysis of carbapenemase-producing Escherichia coli isolates

Jomehzadeh¹,

Jahangirimehr

Chegeni³

2022

PLoS ONE

Self Cite

View full text Add to dashboard Cite

Carbapenem-resistant Escherichia coli has emerged as a major public health issue across the world. This study was aimed to determine the virulence content and phylogenetic groups of carbapenemase-producing E. coli isolates in southwest Iran. One hundred and fifty-two non-duplicate E. coli isolates were collected from various clinical samples. Antibiotic susceptibility and minimum inhibitory concentrations (MIC) were determined according to the Clinical and Laboratory Standards Institute (CLSI) guidelines by Kirby-Bauer disc diffusion and agar dilution methods. Phenotypic screening of carbapenemase enzymes was performed by modified Hodge test (MHT). Detection of carbapenemase genes, phylogenetic groups, and virulence-associated genes were also performed by the PCR assay. The highest and lowest resistance rates were observed against mezlocillin (70.4%) and doripenem (13.1%), respectively. Out of 28 isolates that were resistant to carbapenem antibiotics, 12 (7.9%) strains were phenotypically carbapenemase producers. The blaOXA-48 was the predominant carbapenemase gene, detected in 58.3% of isolates, followed by blaIMP (41.7%) and blaNDM (8.3%). None of the isolates harbored blaVIM and blaKPC genes. Among the twelve carbapenemase-producing strains, urinary isolates were mostly classified into B2 (41.7%) and D (25%) phylogenetic groups, while other clinical isolates belonged to B1 (25%) and A (8.3%) groups. The frequency of virulence-associated genes was also investigated in all isolates and ranged from 6.6% for hly to 75% for fimA. The emergence of carbapenemase-producing strains is a growing concern to public health. Therefore, the proper implementation of monitoring programs is crucial for limiting their dissemination.

show abstract

“…Another work reported by Jomehzadeh et al demonstrated that enteropathogenic Escherichia coli (EPEC), which is one of the most important diarrheagenic agents among infants under 5 years in developing countries, is in relation with the distribution of class 1 integrons and ESBLs in highly prevalent EPEC strains. Moreover, the results revealed that continuous monitoring of the emergence and expansion of MDR in EPEC strains is necessary to decrease the incidence of infectious disease [ 109 ].…”

Section: Guideline Recommendation For Treatment Of Esbl Infectionsmentioning

confidence: 99%

Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Bacteria in Trinidad and Tobago

Akpaka

Vaillant

Wilson

et al. 2021

International Journal of Microbiology

View full text Add to dashboard Cite

Gram-negative bacterial infections are a global health problem. The production of beta-lactamase is still the most vital factor leading to beta-lactam resistance. In Trinidad and Tobago, extended spectrum beta-lactamase (ESBL) production has been detected and reported mainly in the isolates of Klebsiella pneumoniae and Escherichia coli and constitutes a public health emergency that causes high morbidity and mortality in some patients. In this literature review, the authors cover vast information on ESBL frequency and laboratory detection using both conventional and molecular methods from clinical data. The aim is to make the reader reflect on how the actual knowledge can be used for rapid detection and understanding of the spread of antimicrobial resistance problems stemming from ESBL production among common Gram-negative organisms in the health care system.

show abstract

The first evaluation relationship of integron genes and the multidrug-resistance in class A ESBLs genes in enteropathogenic Escherichia coli strains isolated from children with diarrhea in Southwestern Iran

Cited by 7 publications

References 31 publications

Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features

Mucosa-Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features

Virulence-associated genes analysis of carbapenemase-producing Escherichia coli isolates

Extended Spectrum Beta-Lactamase (ESBL) Produced by Gram-Negative Bacteria in Trinidad and Tobago

Contact Info

Product

Resources

About