The first Entamoeba moshkovskii molecular detection in Egypt

El-Badry, Ayman A.; Abu-Sarea, Enas Y.; Mahmoud, Amany H.; Ghieth, Marwa A.; Ismail, Mousa A. M.

doi:10.1007/s00580-018-2878-z

Cited by 5 publications

(7 citation statements)

References 19 publications

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“…In the present study, the overall detected coproscopic prevalence of Entamoeba species was 30 (22.6%) which were confirmed by multiplex PCR and showed that 19 (63.3%), 7 (23.4%) and 4 (13.3%) of the subjects were infected with E. dispar, E. histolytica, and E.moshkovaskii respectively. The predominant prevalence of E. dispar in the present study is close to most previous studies carried out in Egypt (El-Badry et al, 2019;Abozahra et al, 2020), as well as other countries (Fallah et al, 2014;Zaki and Clark, 2001). Contradictory, a study conducted in United Arab Emirates where E. histolytica was more prevalent (ElBakri et al, 2013).…”

Section: Discussionsupporting

confidence: 89%

“…The extracted DNA was stored at − 20 °C until processed. All DNA extracts were amplified by multiplex PCR (m-PCR) as described by El-Badry et al, (2019) to detect E. histolytica, E. dispar, and E. moshkovskii species. M-PCR was targeting small subunit (SSU) rRNA gene using the following species-specific primers EntaF (common Entamoeba forward primer): 5′-ATG CAC GAG AGC GAA AGC AT-3′, EhR (E. histolytica reverse primer): 5′-GAT CTA GAA ACA ATG CTT CTC T-3′, EdR (E. dispar reverse primer): 5′-CAC CAC TTA CTA TCC CTA CC-3′, EmR (E.moshkovskii reverse primer):5′-TGA CCG GAG CCA GAG ACA T-3′.…”

Section: Dna Extraction and Copro-multiplex Pcr Assaymentioning

confidence: 99%

“…A 1.5% agarose gel stained with ethidium bromide was used to visualize obtained PCR products under a UV light system. PCR in the presence of E.histolytica produced a 167-bp, E.dispar produced a 753-bp, and E.moshkovskii produced a 579-bp (Hamzah et al, 2006;El-Badry et al, 2019).…”

Section: Dna Extraction and Copro-multiplex Pcr Assaymentioning

confidence: 99%

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Molecular prevalence of Entamoeba species in a cohort of Egyptians

Ibrahim

Hassan

Nasr

et al. 2022

ijhs

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Background and objective: Intestinal and extraintestinal amebiasis is caused by the protozoan parasite Entamoeba (E.) histolytica, it is of considerable morbidity and mortality in developing countries. E.histoltica complex species includes E.histolytica, E. moshkovskii and E. dispar are morphologically indistinctable. The current study goal was to use molecular assays to detect the true prevalence of E. histolytica complex species among a cohort of Egyptians. Methods: A single stool specimen was collected from 133 patients, examined coproscopically before and after concentration. DNA was extracted from microscopically positive stool specimens for E. histolytica complex species were molecularly idenitified using multiplex PCR. Results: The coroscopic prevalence of intestinal parasites was 51.1% (68/133) of them 30 cases had E.histolytica complex (22.6%; 30/133). Among coproscopically positive samples, E. dispar was the most common parasite (63.3%; 19/30), followed by E. histolytica (23.4%; 7/30) and E. moshkovskii (13.3%; 4/30). There was statistical significance association between sociodemographic characteristics and Entamoeba species in asymptomatic individuals, while in the symptomatic individual, only age groups were stastically significant. Conclusion: E.dispar is the predominant Entamoeba species among studied individuals. There is a need for molecular diagnosis of Entamoeba to determine the true prevalence of E. histolytica and avoid overmedication.

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Section: Discussionsupporting

confidence: 89%

Section: Dna Extraction and Copro-multiplex Pcr Assaymentioning

confidence: 99%

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Molecular prevalence of Entamoeba species in a cohort of Egyptians

Ibrahim

Hassan

Nasr

et al. 2022

ijhs

Self Cite

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“…Symptoms of granulomatous amoebic encephalitis: headache, stiff neck or head and neck pain with neck movement, sensitivity to light, nausea, vomiting, tired, low temperature fever, behavioral changes, seizures, weight loss, partial paralysis, difficulties in pronunciation and a difficulty walking [50]. [27] Taher et al [27] Cryptosporidium hominis/parvum Elfadaly et al [28] Shehata et al [32] Entamoeba histolytica Elfadaly et al [28] El-Badry et al [33] Giardia intestinalis Elfadaly et al [28] Shehata et al [32] Naegleria…”

Section: Granulomatous Amoebic Encephalitismentioning

confidence: 99%

Parasitic Contamination of Drinking Water and Egyptian Standards for Parasites in Drinking Water

Hikal¹

2020

OJE

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Water is life and access to clean drinking water is a fundamental human right. For human health, drinking water standards and guidelines have been developed to ensure their quality. Waterborne parasites are a great challenge and cause many diseases for humans. Thus, continuous monitoring of drinking water from the competent authorities in search of pollutants is required. The safety of drinking water for the growing population can be ensured by the correct use of water treatment technologies. Cryptosporidium is the first parasite to cause concern to health officials in the world. Also, Naegleria fowleri causes fatal cerebral infection, Acanthamoeba species that cause both cerebral and corneal disease; and Giardia which has heightened world concerns because of its severity. This study is based on highlighting on drinking water quality guidelines and standards published by the World Health Organization and some countries, especially Egypt. A growing concern globally after the scientists revealed the effects of polluted drinking water. So, a large number of the population is at risk of water pollution for the purpose of human access to clean drinking water and other uses.

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“…A facilitating marker is the presence of ingested red blood cells in trophozoite forms indicating E. histolytica spp. [8,9] . Recently, species identification can be achieved by serological [10] or copro immunological techniques [8,11] but with certain limitations because the three species share many identical alleles and similar immunogenic effects.…”

Section: Introductionmentioning

confidence: 99%

Association between molecularly detected Entamoeba species and fecal calprotectin level among a cohort of diarrheic patients

Morsy

2022

Parasitologists United Journal

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Background: Differentiation between Entamoeba species can be molecularly achieved, especially since E. histolytica has been linked to high morbidity and mortality. Fecal calprotectin (FC) as an inflammatory marker may be linked to amoebiasis. Objective: The present study aimed at investigating the association of calprotectin in amoebiasis. Patients and Methods: Stool samples were collected from 294 patients attending Internal Medicine Outpatient Clinic in Kafrelsheikh University, Egypt; suffering from diarrhea with or without other gastrointestinal manifestations. Samples were subjected to coproscopy, quantification of fecal calprotectin level and multiplex nested PCR for Entamoeba species differentiation. Results: Detection rate of E. histolytica complex was 16.6% by microscopic examination and only 14.6% proved to be positive by nested multiplex polymerase chain reaction (PCR): E. histolytica (3.7%), E. dispar (6.4%) and E. moshkovskii (4.5%). Statistical analysis including several variables showed no significance except for the presence of blood and mucus. Fecal calprotectin was positive in 10.5% of the study population and 81.8% in association with E. histolytica indicating intestinal inflammation. Frequency of males infected with E. histolytica and E. dispar was higher than in females who showed a higher infection rates with E. moshkovskii. Conclusion:The association between E. histolytica and positivity of FC level is a crucial indicator for disease severity and efficacy of therapeutic regimen.

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The first Entamoeba moshkovskii molecular detection in Egypt

Cited by 5 publications

References 19 publications

Molecular prevalence of Entamoeba species in a cohort of Egyptians

Molecular prevalence of Entamoeba species in a cohort of Egyptians

Parasitic Contamination of Drinking Water and Egyptian Standards for Parasites in Drinking Water

Association between molecularly detected Entamoeba species and fecal calprotectin level among a cohort of diarrheic patients

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