The first detection of Nosema ceranae (Microsporidia) in the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae)

Cilia, Giovanni; Cardaio, Ilaria; Santos, Pedro Emanuel Jarmela dos; Nanetti, Antonio

doi:10.1007/s13592-018-0589-8

Cited by 19 publications

(15 citation statements)

References 46 publications

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“…To date, only a few instances of individual SHBs bearing bee pathogens have been reported. This is the case for samples from Mexico (positive for L. passim , Apis mellifera filamentous virus (AmFV), C. bombi , Ascosphera apis , and Nosema ceranae [ 22 ]), Florida (positive for N. ceranae [ 20 ]), and other areas of the USA (positive for DWV, SBV and P. larvae [ 19 , 20 , 22 , 24 ]).…”

Section: Discussionmentioning

confidence: 99%

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Detection of Lotmaria passim, Crithidia mellificae and Replicative Forms of Deformed Wing Virus and Kashmir Bee Virus in the Small Hive Beetle (Aethina tumida)

2021

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Knowledge regarding the honey bee pathogens borne by invasive bee pests remains scarce. This investigation aimed to assess the presence in Aethina tumida (small hive beetle, SHB) adults of honey bee pathogens belonging to the following groups: (i) bacteria (Paenibacillus larvae and Melissococcus plutonius), (ii) trypanosomatids (Lotmaria passim and Crithidia mellificae), and (iii) viruses (black queen cell virus, Kashmir bee virus, deformed wing virus, slow paralysis virus, sacbrood virus, Israeli acute paralysis virus, acute bee paralysis virus, chronic bee paralysis virus). Specimens were collected from free-flying colonies in Gainesville (Florida, U.S.A.) in summer 2017. The results of the molecular analysis show the presence of L. passim, C. mellificae, and replicative forms of deformed wing virus (DWV) and Kashmir bee virus (KBV). Replicative forms of KBV have not previously been reported. These results support the hypothesis of pathogen spillover between managed honey bees and the SHB, and these dynamics require further investigation.

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Section: Discussionmentioning

confidence: 99%

“…Adult SHBs invade colonies, where they feed, thrive, and reproduce. This allows contact between SHBs and other bee pests and pathogens [ 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Lotmaria passim, Crithidia mellificae and Replicative Forms of Deformed Wing Virus and Kashmir Bee Virus in the Small Hive Beetle (Aethina tumida)

2021

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“…Based on ultra-structural and molecular investigations, a new Nosema species in Apis mellifera , namely N. neumanni (Chemurot et al 2017), was described in Uganda in 2017 [ 19 ]. The importance of N. ceranae spillover is not limited to the honey bee but also to other insect species like the bee-eater Merops apiaster [ 20 ], the South American native bumblebee, Bombus brasiliensis (Lepeletier, 1836) [ 21 , 22 ], stingless bees and social wasps [ 23 ], solitary bees [ 24 ], the small hive beetle, Aethina tumida (Murray, 1867) [ 25 ], etc. Numerous studies have indicated that N. ceranae has become a worldwide distributed microsporidian pathogen, including in Central Italy [ 26 , 27 ], Croatia [ 28 ], Lithuania [ 29 ], etc.…”

Section: Introductionmentioning

confidence: 99%

The Herbal Supplements NOZEMAT HERB® and NOZEMAT HERB PLUS®: An Alternative Therapy for N. ceranae Infection and Its Effects on Honey Bee Strength and Production Traits

2021

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Honey bees (Apis mellifera L.) are the most effective pollinators for different crops and wild flowering plants, thus maintaining numerous ecosystems in the world. However, honey bee colonies often suffer from stress or even death due to various pests and diseases. Among the latter, nosemosis is considered to be one of the most common diseases, causing serious damage to beekeeping every year. Here, we present, for the first time, the effects from the application of the herbal supplements NOZEMAT HERB® (NH) and NOZEMAT HERB PLUS® (NHP) for treating N. ceranae infection and positively influencing the general development of honey bee colonies. To achieve this, in autumn 2019, 45 colonies were selected based on the presence of N. ceranae infections. The treatment was carried out for 11 months (August 2019–June 2020). All colonies were sampled pre- and post-treatment for the presence of N. ceranae by means of light microscopy and PCR analysis. The honey bee colonies’ performance and health were evaluated pre- and post-treatment. The obtained results have shown that both supplements have exhibited statistically significant biological activity against N. ceranae in infected apiaries. Considerable enhancement in the strength of honey bee colonies and the amount of sealed workers was observed just one month after the application of NH and NHP. Although the mechanisms of action of NH and NHP against N. ceranae infection are yet to be completely elucidated, our results suggest a new holistic approach as an alternative therapy to control nosemosis and to improve honey bee colonies’ performance and health.

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“…Besides A. cerana and A. mellifera , the microsporidium was reported in several other hymenopteran species, and was also found in regurgitated pellets of bee-eaters [ 12 , 13 , 14 , 15 , 16 ]. It was also found in small hive beetles ( Aethina tumida) [ 17 ], although the possibility of transmission to hymenopterans remains unclear [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Api-Bioxal® and ApiHerb® Treatments against Nosema ceranae Infection in Apis mellifera Investigated by Two qPCR Methods

Cilia

Garrido²,

Bonetto

et al. 2020

Veterinary Sciences

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Nosema ceranae is a worldwide distributed midgut parasite of western honey bees, leading to dwindling colonies and their collapse. As a treatment, only fumagillin is available, causing issues like resistance and hampered bee physiology. This study aimed to evaluate ApiHerb® and Api-Bioxal® as treatments against N. ceranae. The efficacy was tested using two qPCR methods based on the 16S rRNA and Hsp70 genes. In addition, these methods were compared for their aptitude for the quantification of the infection. For this, 19 colonies were selected based on the presence of N. ceranae infections. The colonies were divided into three groups: treated with ApiHerb, Api-Bioxal with previous queen caging and an untreated control. All colonies were sampled pre- and post-treatment. The bees were analyzed individually and in duplicate with both qPCR methods. All bees in the pre-treatment tested positive for N. ceranae. Both treatments reduced the abundance of N. ceranae, but ApiHerb also decreased the prevalence of infected bees. Analysis with the 16S rRNA method resulted in several orders of magnitude more copies than analysis with the Hsp70 method. We conclude that both products are suitable candidates for N. ceranae treatment. From our analysis, the qPCR method based on the Hsp70 gene results as more apt for the exact quantification of N. ceranae as is needed for the development of veterinary medicinal products.

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The first detection of Nosema ceranae (Microsporidia) in the small hive beetle, Aethina tumida Murray (Coleoptera: Nitidulidae)

Cited by 19 publications

References 46 publications

Detection of Lotmaria passim, Crithidia mellificae and Replicative Forms of Deformed Wing Virus and Kashmir Bee Virus in the Small Hive Beetle (Aethina tumida)

Detection of Lotmaria passim, Crithidia mellificae and Replicative Forms of Deformed Wing Virus and Kashmir Bee Virus in the Small Hive Beetle (Aethina tumida)

The Herbal Supplements NOZEMAT HERB® and NOZEMAT HERB PLUS®: An Alternative Therapy for N. ceranae Infection and Its Effects on Honey Bee Strength and Production Traits

Effect of Api-Bioxal® and ApiHerb® Treatments against Nosema ceranae Infection in Apis mellifera Investigated by Two qPCR Methods

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