The first detection of <i>Anaplasma capra</i>, an emerging zoonotic <i>Anaplasma</i> sp., in erythrocytes

Peng, Yongshuai; Lu, Chenyang; Yan, Ye; Shi, Ke; Chen, Qian; Zhao, Cong; Wang, Rongjun; Zhang, Longxian; Jian, Fuchun; Ning, Changshen

doi:10.1080/22221751.2021.1876532

Cited by 21 publications

(11 citation statements)

References 40 publications

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“…Gram-negative obligatory in bacteria's group belongs to the Anaplasma genus having many members, e.g. Anaplasma ovis, Anaplasma bovis, and Anaplasma marginale [10]. Even though they are known as the pathogens who live inside the cells are called as intraerythrocytic, Anaplasma phagocytophilum and Anaplasma bovis attack the monocytes and neutrophil granulocytes respectively.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Prevalence of Anaplasma Marginale in Cattle and Identification of Rhipicephalus, Boophilus, Hyalomma, And Amblyomma Ticks from District Mirpurkhas and Hyderabad

Baloch¹,

Arijo²,

Sultana³

et al. 2023

biosight

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Introduction: Anaplasma marginal is a rickettsial organism and is the causative agent in cattle anaplasmosis. This Parasite attacks the red blood cells after infection of sensitive bovine and is transmitted by ticks. Methodology: The study was aimed to detect Anaplasma marginale through PCR the prevalence of four genera (Rhipicephalus, Boophilus, Hyalomma, and Amblyomma) of ticks, twelve hundred animals were observed, 560 samples of blood randomly taken from cattle that possesses ticks on their body and these samples were shifted to the Laboratory of Parasitology, Department of Veterinary Parasitology, Sindh Agriculture University, Tandojam. The infected cattle exhibited clinical signs such as Temperature, decrease in appetite, nausea, and chills. The study was conducted over a period of one year from November 2020 to November 2021. Results: The results of the study showed a high rate of Anaplasma infection (88.33%) and a prevalence of ticks at Mirpurkhas. Month-wise data reveals that the maximum infection was shown in the month of June and the minimum in the month of November at District Mirpurkhas, while at Hyderabad, the month-wise data indicates the Maximum infection rate in the month of May, and the minimum infection was found in the month of November. Conclusion: Ticks collected from two districts of Mirpurkhas and Hyderabad proceeded for identification of Tick’s genus, and the mostly ticks which were infested on animals concerned with the Hyalomma genus (26.11%) Amblyomma genus (25.37%), Boophilus genus (24.62%), and Rhipicephalus genus (23.88%).

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Section: Introductionmentioning

confidence: 99%

“…new zoonotic diseases, including other species of Anaplasma in the prokaryotic lists with permanent nomenclature (LPSN), but not legally published. [10].…”

Section: Introductionmentioning

confidence: 99%

Molecular Prevalence of Anaplasma Marginale in Cattle and Identification of Rhipicephalus, Boophilus, Hyalomma, And Amblyomma Ticks from District Mirpurkhas and Hyderabad

Baloch¹,

Arijo²,

Sultana³

et al. 2023

biosight

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“…Anaplasma is the causative agent of anaplasmosis and is distributed globally, infecting a broad range of hosts. Small ruminants can get infected with several species, including A. marginale , A. ovis , A. phagocytophilum , and the newly discovered emerging pathogen A. capra [ 3 , 15 , 16 ]. Infected animals may experience fever, hemolytic anemia, loss of appetite, weight loss, and fatigue, which translate to reduced milk production in dairy small ruminants [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

et al. 2022

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Goats are key livestock animals and goat raising is an income-generating venture for smallholder farmers, supporting agricultural development in many parts of the world. However, goat production is often limited by various factors, such as tick-borne diseases. Goat piroplasmosis is a disease caused by apicomplexan parasites Babesia spp. and Theileria spp., while anaplasmosis is caused by bacterial Anaplasma spp. In the Philippines, the presence of Babesia, Theileria, and Anaplasma has not been reported in goats. In this study, DNA obtained from goats were molecularly screened for Babesia/Theileria and Anaplasma. Of 396, 77.02% (305/396) and 38.64% (153/396) were positive for piroplasma and Anaplasma using PCR assays targeting the 18S rRNA and 16S rRNA genes, respectively. Similarly, Babesia ovis was detected in six samples (1.52%). Representative Babesia/Theileria sequences shared 89.97–97.74% identity with each other and were most closely related to T. orientalis, T. annulata, and Theileria spp. Meanwhile, Anaplasma 16SrRNA sequences were related to A. odocoilei, A. platys, and A. phagocytophilum. This is the first molecular identification of B. ovis, Theileria spp., and Anaplasma spp. in goats from the Philippines.

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“…This was established by Kitamura et al in 1987 [ 19 ]. We recently reported that A. capra could infect goat erythrocytes [ 20 ]. To verify the ability of A. capra to infect human cells, A. capra were inoculated in human erythrocytes, HL-60, and TF-1 cell lines in vitro .…”

Section: Introductionmentioning

confidence: 99%

The Novel Zoonotic Pathogen, Anaplasma capra, Infects Human Erythrocytes, HL-60, and TF-1 Cells In Vitro

Peng

Yan

et al. 2021

Pathogens

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Anaplasma capra, a species of the family Anaplasmataceae, is zoonotic tick-borne obligate intracellular bacteria. There have been no reports of human infection with this pathogen since 2015. Therefore, the zoonotic characteristics of A. capra need to be further studied. To verify the ability of A. capra to infect human cells, A. capra were inoculated in human erythrocytes, HL-60, and TF-1 cell lines in vitro. Cell smears were taken after inoculation, using Giemsa staining, transmission electron microscope (TEM), chromogenic in situ hybridization and immunocytochemistry for detection. In the Giemsa staining, many dark colored corpuscles or purple granules were seen in the inoculated erythrocytes, HL-60, and TF-1 cells. The results of chromogenic in situ hybridization show that there were brown precipitates on the surface of most erythrocytes. Immunocytochemistry results show many dark brown vacuolar structures or corpuscles in the cytoplasm of erythrocytes, HL-60, and TF-1 cell lines. The A. capra morulae were seen in the cytoplasm of both HL-60 and TF-1 in TEM, and their diameter was about 295–518 nm. Both dense-cored (DC) and reticulate cell (RC) form morulae could be seen. This study confirmed the ability of A. capra to infect human erythrocytes, HL-60, and TF-1. This study is of profound significance in further verifying the zoonotic characteristics of the pathogen and for establishing an in vitro cultivation model.

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The first detection of Anaplasma capra, an emerging zoonotic Anaplasma sp., in erythrocytes

Cited by 21 publications

References 40 publications

Molecular Prevalence of Anaplasma Marginale in Cattle and Identification of Rhipicephalus, Boophilus, Hyalomma, And Amblyomma Ticks from District Mirpurkhas and Hyderabad

Molecular Prevalence of Anaplasma Marginale in Cattle and Identification of Rhipicephalus, Boophilus, Hyalomma, And Amblyomma Ticks from District Mirpurkhas and Hyderabad

First Molecular Identification of Babesia, Theileria, and Anaplasma in Goats from the Philippines

The Novel Zoonotic Pathogen, Anaplasma capra, Infects Human Erythrocytes, HL-60, and TF-1 Cells In Vitro

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