The first crenarchaeon capable of growth by anaerobic carbon monoxide oxidation coupled with H2 production

Kochetkova, Tatiana V.; Mardanov, Andrey V.; Sokolova, T. G.; Bonch-Osmolovskaya, E. A.; Kublanov, Ilya V.; Kevbrin, V. V.; Beletsky, Alexey V.; Равин, Н. В.; Lebedinsky, Alexander V.

doi:10.1016/j.syapm.2020.126064

Cited by 8 publications

(12 citation statements)

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“…The phylogenetic position of strain 1910b T as well as the average nucleotide identity (ANI) values between 1910b T and validly published T. pendens and T. uzonense placed strain 1910b T in a separate species of the genus (Kochetkova et al, 2019). Taking into account the phenotypic properties of 1910b T (cellulose, β-glucan, cellobiose, mannose, lactose and pyruvate utilization), we propose a novel species of the genus, T. adornatum sp.…”

Section: Cell Morphology and Growth Characteristicsmentioning

confidence: 58%

“…Until now, cellulose was not among the substrates utilized by representatives of the family except for the weak growth on Avicel of Thermofilum sp. strain 1505 (Kochetkova et al, 2019). In this work, we demonstrate the novel species Thermofilum adornatum sp.…”

Section: Introductionmentioning

confidence: 60%

“…The utilization of various organic substrates was tested under standard growth conditions under N 2 in the gas phase (80 • C, pH 5.5). Other Thermofilum species, such as T. uzonense strain 1807-2 T (Toshchakov et al, 2015) and strain 1505 (Kochetkova et al, 2019), were used for comparative growth experiments and were taken from the laboratory collection.…”

Section: Culture Characterization and Cultivationmentioning

confidence: 99%

“…The genome of T. adornatum 1910b T contained 39 genes, which were predicted to encode CAZymes (Lombard et al, 2014). Seventeen of them encoded putative glycosidases (GH), 16 Kochetkova et al, 2019Zillig et al, 1983Anderson et al, 2008Toshchakov et al, 2015 All strains are strict anaerobes and capable of fermenting yeast extract and peptone. ND, no data available; +, growth; −, no growth (no difference with the control); (+g), the genes, encoding enzymes, presumably involved in degradation of these substrates were found by genome analysis; * , our data.…”

Section: Genomic Analysis Of Sugar Metabolismmentioning

confidence: 99%

“…Representatives of Thermofilaceae are moderately acidophilic and hyperthermophilic crenarchaea are obligately dependent on various components of cells or culture broths of other Crenarchaeota, yet they are capable of utilizing polysaccharides as sources of carbon and energy (Zillig et al, 1983;Toshchakov et al, 2015;Kochetkova et al, 2019). Until now, cellulose was not among the substrates utilized by representatives of the family except for the weak growth on Avicel of Thermofilum sp.…”

Section: Introductionmentioning

confidence: 99%

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Novel Hyperthermophilic Crenarchaeon Thermofilum adornatum sp. nov. Uses GH1, GH3, and Two Novel Glycosidases for Cellulose Hydrolysis

et al. 2020

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A novel hyperthermophilic, anaerobic filamentous archaeon, Thermofilum adornatum strain 1910b T , is capable of growing with cellulose as its sole carbon and energy source. This strain was isolated from a terrestrial hot spring in Kamchatka, Russia. The isolate 1910b T grew optimally at a temperature of 80 • C and a pH of 5.5-6.0, producing cell-bound inducible cellulases. During genome analysis, genes, encoding various glycosidases (GHs) involved in oligo-and polysaccharide hydrolysis and genes for the fermentation of sugars were identified. No homologs of currently known cellulase families were found among the GHs encoded by the 1910b T genome, suggesting that novel proteins are involved. To figure this out, a proteomic analysis of cells grown on cellulose or pyruvate (as a control) was performed. Both in-depth genomic and proteomic analyses revealed four proteins (Cel25, Cel30, Cel40, and Cel45) that were the most likely to be involved in the cellulose hydrolysis in this archaeon. Two of these proteins (Cel30 and Cel45) were hypothetical according to genome analysis, while the other two (Cel25 and Cel40) have GH3 and GH1 domains, respectively. The respective genes were heterologously expressed in Escherichia coli BL21 (DE3), and enzymatic activities of recombinant proteins were measured with carboxymethyl cellulose (CMC), Avicel and cellobiose as substrates. It was revealed that the Cel30 and Cel25 proteins were likely exoglucanases with side beta-glucosidase and endoglucanase activities, that Cel40 was a multifunctional glucanase capable of hydrolyzing beta-1,4-glucosides of various lengths, and that Cel45 was an endoglucanase with side exoglucanase activity. Taking into account that the cellulolytic activity of T. adornatum 1910b T surface protein fractions was inducible, that recombinant Cel25 and Cel30 were much less active than Cel40 and Cel45, and that their gene expressions were (almost) non-induced by CMC, we suggest that Cel40 and Cel45 play a major role in the degradation of cellulose, while Cel25 and Cel30 act only as accessory enzymes.

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Section: Cell Morphology and Growth Characteristicsmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 60%