The first comprehensive description of the expression profile of genes involved in differential body growth and the immune system of the Jeju Native Pig and miniature pig

Ghosh, Mrinmoy; Sharma, Neelesh; Gera, Meeta; Kim, Nameun; Sodhi, Simrinder Singh; Pulicherla, K. K.; Huynh, Do Luong; Kim, Dae Cheol; Zhang, Jiao Jiao; Kwon, Taeho; Ma, Kyung Tak; Lee, Hak Kyo; Song, Ki-Duk; Jeong, Dong-Kee

doi:10.1007/s00726-018-2685-5

Cited by 8 publications

(17 citation statements)

References 67 publications

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“…In our study, expression levels of these genes were different depending on culture conditions of JBP muscle cells. Expression levels of the six down-regulated genes reported by Ghosh et al [1] were signi cantly higher under a culture condition without growth factors (Fig. 4A).…”

Section: Gene Expression Pattern In Jbp Muscle Cellsmentioning

confidence: 62%

“…4A). On the other hand, expression levels of two up-regulated genes (POSTN and COL21A1) reported by Ghosh et al [1] were signi cantly higher under culture conditions containing growth factors, although…”

Section: Gene Expression Pattern In Jbp Muscle Cellsmentioning

confidence: 73%

“…Many previous studies have been performed in an attempt to identify genes affecting muscle development and growth. Ghosh et al [1] have selected genes related to muscle growth that are differentially expressed from muscle tissues of Jeju native pig and miniature pig. Among those genes reported by Ghosh et al [1], 6 down-regulated genes and 4 up-regulated genes in Jeju native pigs were analyzed at cellular level in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Pigs are important economically in the livestock industry. They are also excellent biomedical animal models [1]. Because sizes and physiological systems of pigs are similar to those of human, pigs have been used as biomedical models for human studies [2].…”

Section: Introductionmentioning

confidence: 99%

“…We demonstrated that the expression of MyoD in JBP muscle cells was up-regulated in EGF and bFGF containing medium. Ghosh et al [1] have identi ed ten growth-related genes. We checked changes of these ten growth related genes in JBP muscle cells.…”

Section: Introductionmentioning

confidence: 99%

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Growth factors improve the proliferation of Jeju black pig muscle cells by regulating MyoD and growth-related genes

Park

Lee

Song

et al. 2020

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Background Jeju black pig (JBP), one of Korean native pig breeds, has excellent meat quality but grow slowly. The growth rate of pigs is related to differentiation and proliferation of muscle cells regulated by growth factors and expression of growth-related genes. However, few studies have determined the effect of growth factors on the proliferation of porcine muscle cells. Thus, the objective of this study was to establish optimal culture conditions of JBP muscle cells and determine the relationship of various factors involved in muscle growth with the proliferation of JBP muscle cells. Results We established a muscle cell line from JBP embryos and optimized its culture conditions. These muscle cells were positive for MyoD, but not Pax7. The proliferation rate of these muscle cells was significantly higher in a culture medium containing bFGF and EGF + bFGF than that without a growth factor or containing EGF alone. Treatment with EGF and bFGF significantly induced the expression of MyoD protein, an important transcription factor in muscle cells. Moreover, we checked changes of the expression of growth-related genes in JBP muscle cells by presence or absence of growth factors. Expression level of COL21A1 gene was changed only when EGF and bFGF were both added to culture media for JBP muscle cells. Conclusions Concurrent use of EGF and bFGF increased the expression of MyoD protein, thus regulating the proliferation of JBP muscle cells and the expression of growth-related genes.

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Section: Gene Expression Pattern In Jbp Muscle Cellsmentioning

confidence: 62%

Section: Gene Expression Pattern In Jbp Muscle Cellsmentioning

confidence: 73%