The first complete genomic structure of Butyrivibrio fibrisolvens and its chromid

Abstract

Butyrivibrio fibrisolvens forms part of the gastrointestinal microbiome of ruminants and other mammals, including humans. Indeed, it is one of the most common bacteria found in the rumen and plays an important role in ruminal fermentation of polysaccharides, yet, to date, there is no closed reference genome published for this species in any ruminant animal. We successfully assembled the nearly complete genome sequence of B. fibrisolvens strain INBov1 isolated from cow rumen using Illumina paired-end reads, 454 Roche single-end and mate pair sequencing technology. Additionally, we constructed an optical restriction map of this strain to aid in scaffold ordering and positioning, and completed the first genomic structure of this species. Moreover, we identified and assembled the first chromid of this species (pINBov266). The INBov1 genome encodes a large set of genes involved in the cellulolytic process but lacks key genes. This seems to indicate that B. fibrisolvens plays an important role in ruminal cellulolytic processes, but does not have autonomous cellulolytic capacity. When searching for genes involved in the biohydrogenation of unsaturated fatty acids, no linoleate isomerase gene was found in this strain. INBov1 does encode oleate hydratase genes known to participate in the hydrogenation of oleic acids. Furthermore, INBov1 contains an enolase gene, which has been recently determined to participate in the synthesis of conjugated linoleic acids. This work confirms the presence of a novel chromid in B. fibrisolvens and provides a new potential reference genome sequence for this species, providing new insight into its role in biohydrogenation and carbohydrate degradation.

tRNA Queuosine Modification Enzyme Modulates the Growth and Microbiome Recruitment to Breast Tumors

“…The source of the bacteria in tumors may shed light on breast tumor development. B. fibrisolvens belongs to the genus Butyrivibrio and is part of the gastrointestinal microbiome of mammals and ubiquitously present in the gastrointestinal tract of many animals, including humans and mice [43]. These bacteria inside the tumors could be translocated from the intestine directly.…”

Metagenomic sequencing identified microbial species in the rumen and cecum microbiome responsible for niacin treatment and related to intramuscular fat content in finishing cattle

“…This was consistent with the previous reports that niacin can avoid lactate accumulation in rumen wall ( Kristensen and Harmon, 2004 ; Luo et al, 2017 ). Niacin also led to an increase in Butyrivibrio fibrisolvens and Roseburia intestinalis , which play an important role in the ruminal fermentation of polysaccharides to produce butyrate ( Tamanai-Shacoori et al, 2017 ; Rodriguez Hernaez et al, 2018 ). These results were consistent with the observation that the KEGG pathways of Carbohydrate digestion and absorption, and Central carbon metabolism in cancer were enriched in niacin group, suggesting the improvement of Carbohydrate metabolism and absorption by niacin.…”

A Comparison Of Fecal Glucocorticoid Metabolite Concentration And Gut Microbiota Diversity In Bonobos (Pan paniscus)

“…and an unknown member of family RF16 associated with the rumen gut microbiota [80]–[82], Prevotella sp., which is associated with plant-rich diets, chronic inflammatory conditions and non-Western diets [67], [83], [84], and a Butyrivibrio sp. a well know butyrate-producing bacteria [85], [86]. These different patterns between the bonobo and western lowland gorilla suggest that there may be species-specific and temporal-specific effects of FGMC on the abundance of specific taxa in primate gut microbiota.…”