The First Chromosomal Characteristics of Nucleolar Organizer Regions and Karyological Analysis of Pink Anemonefish, <i>Amphiprion perideraion</i> (Perciformes, Amphiprioninae)

Abstract

Summary This study examined for the first time the chromosomal characteristics of nucleolar organizer regions (NORs) and karyological analysis of the pink anemonefish, Amphiprion perideraion (Bleeker 1955) from the Andaman Sea, Phuket Province, Thailand. Kidney cell samples were taken from five male and five female fish. Mitotic chromosome preparations were conducted using a standard squash technique as well as taken directly from kidney cells. Metaphase spreads were performed on microscopic slides and then air-dried. Conventional and Ag-NOR banding techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of A. perideraion was 2n=48, and the fundamental number (NF) was 94 in both males and females. Karyotpes were present as 2 large metacentric, 18 large submetacentric, 8 large acrocentric, 10 medium metacentric, 6 medium submetacentric, 2 medium acrocentric and 2 medium telocentric chromosomes. No irregularly sized chromosomes related to sex were observed. The results indicated that the short arm of the large acrocentric chromosome pair No. 19 showed clearly observable NORs. The karyotype formula for A. perideraion is as follows:

Karyotype and Characteristics of Nucleolar Organizer Regions in Longfin Carp, <i>Labiobarbus leptocheilus</i> (Cypriniformes, Cyprinidae)

“…3). This is in accordance with the previous studies in orange-spotted grouper, Epinephelus coioides (Wang et al 2010), Smith s barb, Puntioplites proctozysron (Supiwong et al 2012) and pink anemonefish, Amphiprion perideraion (Supiwong et al 2015). In most species of the family Cyprinidae investigated to date, the single NOR-bearing chromosome pair is conserved.…”

Finding Nemo’s Genes: A chromosome‐scale reference assembly of the genome of the orange clownfishAmphiprion percula

“…Briefly, contigs were clustered into chromosomal groups using a hierarchical clustering algorithm based on the number of read pairs linking scaffolds, with the final number of groups specified as the number of the haploid chromosomes. The haploid chromosome number was set as 24, which is consistent with the observed haploid chromosome number of the Amphiprioninae, as published for A. ocellaris (Arai, Inoue, & Ida, 1976), A. frenatus, (Molina & Galetti, 2004;Takai & Kosuga, 2007), A. clarkii Takai & Kosuga, 2007), A. perideraion (Supiwong et al, 2015) and A. polymnus (Tanomtong et al, 2012). After clustering into chromosomal groups, the scaffolds were ordered based on Hi-C link densities and then oriented with respect to the adjacent scaffolds using a weighted directed acyclic graph of all possible orientations based on the exact locations of the Hi-C links between scaffolds.…”

Finding Nemo’s Genes: A chromosome-scale reference assembly of the genome of the orange clownfish Amphiprion percula

“…), A. frenatus, (Molina & Galetti, 2004; Takai & Kosuga, 2007), A. clarkii(Arai & 201 Inoue, 1976;Takai & Kosuga, 2007), A. perideraion(Supiwong et al, 2015) and A. polymnus 202(Tanomtong et al, 2012). After clustering into chromosomal groups, the scaffolds were 203 ordered based on Hi-C link densities and then oriented with respect to the adjacent scaffolds 204 using a weighted directed acyclic graph of all possible orientations based on the exact 205 locations of the Hi-C links between scaffolds.…”