The First Application of Nanoelectrochemotherapy in Feline Oral Malignant Melanoma Treatment—Case Study

Tunikowska, Joanna; Antończyk, Agnieszka; Rembiałkowska, Nina; Jóźwiak, Łukasz; Novickij, Vitalij; Kulbacka, Julita

doi:10.3390/ani10040556

Cited by 14 publications

(9 citation statements)

References 20 publications

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“…15 , 16 Additionally, with nanosecond pulses, the possibility of thermal damage to the tissue is minimized 5 , 6 due to low energy being transferred to the treated area and electrochemical reactions are reduced. 8 ECT with nanosecond pulses has shown promising results 8 , 17 , 18 , 19 , but the underlying mechanisms of the observed decrease in cell survival and tumor regression remain to be explained.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, nanosecond pulses have been explored in ECT and calcium electroporation and have shown promising results -either tumor regression in vivo or a decrease in cell survival in vitro. 8,[17][18][19][20][21] We have previously reported that nanosecond pulses of an appropriately chosen amplitude in combination with cisplatin decreased cell survival in in vitro assays to the same extent as standard 8 × 100 μs pulses. 8 The aim of our present work was to investigate the underlying mechanisms of ECT with nanosecond pulses and cisplatin in vitro on Chinese hamster ovary CHO and mouse skin melanoma B16F1 cells.…”

Section: Introductionmentioning

confidence: 99%

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Nanosecond electric pulses are equally effective in electrochemotherapy with cisplatin as microsecond pulses

Vižintin

Marković

Ščančar

et al. 2022

Radiology and Oncology

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Background Nanosecond electric pulses showed promising results in electrochemotherapy, but the underlying mechanisms of action are still unexplored. The aim of this work was to correlate cellular cisplatin amount with cell survival of cells electroporated with nanosecond or standardly used 8 × 100 μs pulses and to investigate the effects of electric pulses on cisplatin structure. Materials and methods Chinese hamster ovary CHO and mouse melanoma B16F1 cells were exposed to 1 × 200 ns pulse at 12.6 kV/cm or 25 × 400 ns pulses at 3.9 kV/cm, 10 Hz repetition rate or 8 × 100 μs pulses at 1.1 (CHO) or 0.9 (B16F1) kV/cm, 1 Hz repetition rate at three cisplatin concentrations. Cell survival was determined by the clonogenic assay, cellular platinum was measured by inductively coupled plasma mass spectrometry. Effects on the structure of cisplatin were investigated by nuclear magnetic resonance spectroscopy and high-resolution mass spectrometry. Results Nanosecond pulses equivalent to 8 × 100 μs pulses were established in vitro based on membrane permeabilization and cell survival. Equivalent nanosecond pulses were equally efficient in decreasing the cell survival and accumulating cisplatin intracellularly as 8 × 100 μs pulses after electrochemotherapy. The number of intracellular cisplatin molecules strongly correlates with cell survival for B16F1 cells, but less for CHO cells, implying the possible involvement of other mechanisms in electrochemotherapy. The high-voltage electric pulses did not alter the structure of cisplatin. Conclusions Equivalent nanosecond pulses are equally effective in electrochemotherapy as standardly used 8 × 100 μs pulses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanosecond electric pulses are equally effective in electrochemotherapy with cisplatin as microsecond pulses

Vižintin

Marković

Ščančar

et al. 2022

Radiology and Oncology

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show abstract

“…Another case study outlined the use of nano-ECT as an adjunct after the CO 2 laser surgical removal of the bulk of an oral melanoma in a cat. 19 Nano-ECT is a form of ECT that delivers a pulsed electric field with a duration of nanoseconds rather than microseconds, 20 as is more commonly used, and which was utilised in this case. In that case report the patient was treated with nanosecond pulses and bleomycin.…”

Section: Discussionmentioning

confidence: 99%

Electrochemotherapy as a treatment option for feline nasal melanoma

Little

Macfarlane

2022

Journal of Feline Medicine and Surgery Open Reports

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Case summary A 4-year-old domestic shorthair cat presented to North Downs Specialist Referrals for treatment of a 7 mm melanoma of the nasal planum. Two treatments of electrochemotherapy using intravenous bleomycin as a chemotherapeutic agent were administered. The tumour was no longer visible after treatment. At the 292-day follow-up there was no recurrence at the site and the patient was clinically well. Relevance and novel information There are few data concerning the outcomes of cats with non-ocular feline melanomas and there are currently minimal descriptions in the literature of electrochemotherapy as a treatment option. The available data suggest that nasal melanoma may have a worse prognosis, and that melanomas are locally invasive and have a high propensity for metastasis. The current most effective means for local control is surgery with wide margins. Given the location in this case, this would have required nasal planectomy, while electrochemotherapy provided an effective and possibly more attractive alternative.

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“…Electroporation is still a developing technique and finds usability mainly for drug or gene delivery or in food technology [ 1 , 2 ]. Nanosecond electric pulsed fields (nsPEFs) are mainly applied in food technology known as bio-based industry [ 3 , 4 ], but researchers still demonstrate numerous nsPEFs’ advantages in anticancer protocols [ 5 , 6 , 7 , 8 ]. The nanosecond range of the electric pulses induces various changes in the exposed cells.…”

Section: Introductionmentioning

confidence: 99%

Nanosecond PEF Induces Oxidative Stress and Apoptosis via Proteasomal Activity Inhibition in Gastric Adenocarcinoma Cells with Drug Resistance

Kulbacka

Rembiałkowska

Szewczyk

et al. 2022

IJMS

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Nanosecond (ns) pulsed electric field (PEF) is a technology in which the application of ultra-short electrical pulses can be used to disrupt the barrier function of cell plasma and internal membranes. Disruptions of the membrane integrity cause a substantial imbalance in cell homeostasis in which oxidative stress is a principal component. In the present study, nsPEF-induced oxidative stress was investigated in two gastric adenocarcinoma cell lines (EPG85-257P and EPG85-257RDB) which differ by their sensitivity to daunorubicin. Cells were exposed to 200 pulses of 10 ns duration, with the amplitude and pulse repetition frequency at 1 kHz, with electric field intensity varying from 12.5 to 50 kV/cm. The electroporation buffer contained either 1 mM or 2 mM calcium chloride. CellMask DeepRed visualized cell plasma permeabilization, Fluo-4 was used to visualize internal calcium ions content, and F-actin was labeled with AlexaFluor®488 for the cytoskeleton. The cellular viability was determined by MTT assay. An alkaline and neutral comet assay was employed to detect apoptotic and necrotic cell death. The luminescent method estimated the modifications in GSSG/GSH redox potential and the imbalance of proteasomal activity (chymotrypsin-, trypsin- and caspase-like). The reactive oxygen species (ROS) level was measured by flow cytometry using dihydroethidium (DHE) dye. Morphological visualization indicated cell shrinkage, affected cell membranes (characteristic bubbles and changed cell shape), and the reorganization of actin fibers with sites of its dense concentration; the effect was more intense with the increasing electric field strength. The most significant decrease in cell viability and GSSG/GSH redox potential was noted at the highest amplitude of 50 kV/cm, and calcium ions amplified this effect. nsPEF, particularly with calcium ions, inhibited proteasomal activities, resulting in increased protein degradation. nsPEF increased the percentage of apoptotic cells and ROS levels. The EPG85-257 RDB cell line, which is resistant to standard chemotherapy, was more sensitive to applied nsPEF protocols. The applied nsPEF method disrupted the metabolism of cancer cells and induced apoptotic cell death. The nsPEF ability to cause apoptosis, oxidative stress, and protein degradation make the nsPEF methodology a suitable alternative to current anticancer pharmacological methods.

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The First Application of Nanoelectrochemotherapy in Feline Oral Malignant Melanoma Treatment—Case Study

Cited by 14 publications

References 20 publications

Nanosecond electric pulses are equally effective in electrochemotherapy with cisplatin as microsecond pulses

Nanosecond electric pulses are equally effective in electrochemotherapy with cisplatin as microsecond pulses

Electrochemotherapy as a treatment option for feline nasal melanoma

Nanosecond PEF Induces Oxidative Stress and Apoptosis via Proteasomal Activity Inhibition in Gastric Adenocarcinoma Cells with Drug Resistance

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