The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp. ICE-L

An, Meiling; Zheng, Zhou; Qu, Changfeng; Wang, Xixi; Chen, Hao; Shi, Chongli; Miao, Jun

doi:10.1016/j.mrfmmm.2018.03.004

Cited by 16 publications

(10 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total RNA of P. tricornutum ICE-H was extracted from liquid nitrogen ground algae powder using Trizol reagent (TransGen, China) as described in the instruction (An et al 2018). The RNA was observed by agarose gel.…”

Section: Cloning Of Dna Photolyase Gene and Bioinformatics Analysismentioning

confidence: 99%

“…DNA photolyases recognize and bind to DNA lesions such as CPDs or 6-4PPs and, are usually classified as CPD photolyases or (6-4) photolyases (Brych et al 2016;Zhang et al 2017). Two kinds of photolyases share similar primary sequences and folding structures, but a photolyase repairs one photoproduct and cannot repair another (An et al 2018). Five classes of photolyases have been reported: class I, II and III CPD photolyases, (6-4) photolyase and single-stranded DNA photolyase (DASH Crys) (Ozturk et al 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Miao

et al. 2021

3 Biotech

Self Cite

View full text Add to dashboard Cite

Two gene of class II photolyases, PiPhr1 (1833 bp) and PiPhr2 (1809 bp), from the Antarctic diatom Phaeodactylum tricornutum ICE-H were cloned, the recombinant proteins expressed and purified. The molecular weight of the recombinant photolyases were determined to be 68 kDa with a pI of 9.04 and 68.82 with a pI of 7.31, respectively. Activity studies showed that both the recombinant enzymes were involved in the repair DNA damaged by UV light, that is they were most likely photolyases involved in photorepair of DNA. Further confirmation of this function was demonstrated by the increased expression of PiPhr1 and PiPhr2 after exposure to UV radiation, blue light and dark conditions by qRT-PCR. In summary, PiPhr1 and PiPhr2 were up regulated by UVB irradiation and blue light at 0.5 h and 3 h. Longtime (3 h) exposure to dark also increased the expression of PiPhr1 and PiPhr2. In vitro photoreactivation assays showed that PiPhr1 and PiPhr2 could repair CPDs utilizing blue light. This is the first time CPD Class II photolyase has been reported from Antarctic diatom. These results will add to the knowledge of the diatom CPF family and assist in understanding the functional role of these genes in Antarctic diatoms.

show abstract

Section: Cloning Of Dna Photolyase Gene and Bioinformatics Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Miao

et al. 2021

3 Biotech

Self Cite

View full text Add to dashboard Cite

show abstract

“…The Chlamydomonas sp. ICE-L is a crucial component of Antarctic communities, possessing an efficient DNA photolyase to repair UVB-induced DNA lesions [ 19 , 40 ], which is essential for its survival under high-UVB circumstances. In the present study, CPD photolyase nanoliposomes (CPDNL) from the Antarctic Chlamydomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

Liu

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

UVB radiation is known to trigger the block of DNA replication and transcription by forming cyclobutane pyrimidine dimer (CPD), which results in severe skin damage. CPD photolyase, a kind of DNA repair enzyme, can efficiently repair CPDs that are absent in humans and mice. Although exogenous CPD photolyases have beneficial effects on skin diseases, the mechanisms of CPD photolyases on the skin remain unknown. Here, this study prepared CPD photolyase nanoliposomes (CPDNL) from Antarctic Chlamydomonas sp. ICE-L, which thrives in harsh, high-UVB conditions, and evaluated their protective mechanisms against UVB-induced damage in mice. CPDNL were optimized using response surface methodology, characterized by a mean particle size of 105.5 nm, with an encapsulation efficiency of 63.3%. Topical application of CPDNL prevented UVB-induced erythema, epidermal thickness, and wrinkles in mice. CPDNL mitigated UVB-induced DNA damage by significantly decreasing the CPD concentration. CPDNL exhibited antioxidant properties as they reduced the production of reactive oxygen species (ROS) and malondialdehyde. Through activation of the NF-κB pathway, CPDNL reduced the expression of pro-inflammatory cytokines including IL-6, TNF-α, and COX-2. Furthermore, CPDNL suppressed the MAPK signaling activation by downregulating the mRNA and protein expression of ERK, JNK, and p38 as well as AP-1. The MMP-1 and MMP-2 expressions were also remarkably decreased, which inhibited the collagen degradation. Therefore, we concluded that CPDNL exerted DNA repair, antioxidant, anti-inflammation, and anti-wrinkle properties as well as collagen protection via regulation of the NF-κB/MAPK/MMP signaling pathways in UVB-induced mice, demonstrating that Antarctic CPD photolyases have the potential for skincare products against UVB and photoaging.

show abstract

“…Since actinic keratosis and skin cancer could be prevented and treated with photolyases, it is becoming increasingly important to screen for new photolyases and study their biochemical properties. Several bacteria, fungi, and eukaryotes were found to contain photolyases [ 18 , 19 , 20 ]. A sunscreen extract containing DNA photolyase from Anacystis nidulans exhibited photoprotective effects [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress

Wang

Lei

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Cyclobutane pyrimidine dimers (CPDs) are the main mutagenic DNA photoproducts caused by ultraviolet B (UVB) radiation and represent the major cause of photoaging and skin carcinogenesis. CPD photolyase can efficiently and rapidly repair CPD products. Therefore, they are candidates for the prevention of photodamage. However, these photolyases are not present in placental mammals. In this study, we produced a recombinant photolyase-thymine (rPHO) from Thermus thermophilus (T. thermophilus). The rPHO displayed CPD photorepair activity. It prevented UVB-induced DNA damage by repairing CPD photoproducts to pyrimidine monomers. Furthermore, it inhibited UVB-induced ROS production, lipid peroxidation, inflammatory responses, and apoptosis. UVB-induced wrinkle formation, epidermal hyperplasia, and collagen degradation in mice skin was significantly inhibited when the photolyase was applied topically to the skin. These results demonstrated that rPHO has promising protective effects against UVB-induced photodamage and may contribute to the development of anti-UVB skin photodamage drugs and cosmetic products.

show abstract

The first (6-4) photolyase with DNA damage repair activity from the Antarctic microalga Chlamydomonas sp. ICE-L

Cited by 16 publications

References 33 publications

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Two class II CPD photolyases, PiPhr1 and PiPhr2, with CPD repair activity from the Antarctic diatom Phaeodactylum tricornutum ICE-H

Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

Recombinant Photolyase-Thymine Alleviated UVB-Induced Photodamage in Mice by Repairing CPD Photoproducts and Ameliorating Oxidative Stress

Contact Info

Product

Resources

About