The Firmness of Tomato Fruit in Relation to Polygalacturonase Activity

Hobson, Graeme E.

doi:10.1080/00221589.1965.11514121

Cited by 103 publications

(51 citation statements)

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“…The results are similar to the findings of scientists who worked on the role of PG during fruit development in various kinds of fruits and found a correlation, as PG activity and softening occurs simultaneously in cherry (Barrett and Gonzalez, 1994), date (Hasegawa et al, 1969), durian (Ketsa and Daengkanit, 1999), kiwifruit (Wang et al, 2000), mango (Ketsa et al, 1998), papaya (Paull and Chen, 1983), pear (Ahmed and Labavitch, 1980), pepper (Rao and Paran, 2003;Arancibia and Motsenbocker, 2006), tobacco (Osteryoung et al, 1990), and tomato (Hobson, 1965;Crookes and Grierson, 1983;Huber, 1983;DellaPenna et al, 1987;Bird et al, 1988;Biggs and Handa, 1989;Smith et al, 1990;Fenwick et al, 1996).…”

Section: Discussionsupporting

confidence: 78%

“…Pectins are increasingly solubilized, and thus may contribute to their loss from the cell walls, resulting in softening during ripening (Huber, 1983;Ketsa and Daengkanit, 1999). This suggests that mature fruit may have greater activity of softening enzymes such as PG, contributing to the degradation of pectin cell walls (Hobson, 1965;Hasegawa et al, 1969;Zauberman and Schiffmann-Nadel, 1972;Nagy et al, 1985;Barrett and Gonzalez, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…The softening phenomenon influenced by PG in fruits like apples, cherries, dates, durians, grapes, kiwis, melons, peaches, pears, pineapples, tobacco, and tomatoes during ripening has been studied during the past two decades and substantial evidence has been collected, suggesting that the cell wall enzyme PG plays a major role in the entire ripening process (Hobson, 1965;Hasegawa et al, 1969;Ahmed and Labavitch, 1980;Paull and Chen, 1983;Osteryoung et al, 1990;Barrett and Gonzalez, 1994;Ketsa et al, 1998;Ketsa and Daengkanit, 1999).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Ahmed

Gong²,

Khan³

et al. 2011

Genet. Mol. Res.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Ahmed

Gong²,

Khan³

et al. 2011

Genet. Mol. Res.

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“…Rutgers and Castlemart are considered relatively soft and firm cultivars, respectively. The difference in levels of PG mRNA in each cultivar raises the possibility that PG mRNA levels might be correlated with fruit softening, analogous to the observed correlation between PG protein and fruit softening (6,13 (12).…”

mentioning

confidence: 99%

Polygalacturonase Gene Expression in Rutgers, rin, nor, and Nr Tomato Fruits

DellaPenna¹,

Kates²,

Bennett³

1987

Plant Physiol.

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ABSTRACrPolygacturonse (PG) The ripening of tomato fruit is characterized by a series of coordinated biochemical and physiological changes which collectively contribute to the final texture, color, and flavor ofthe ripe fruit. The softening of tomato fruit during ripening has been studied extensively during the past 20 years and a large body of evidence has accumulated which suggests the cell wall enzyme PG2 plays a major role in this process. Several lines of evidence which support this include: (a) a rough correlation between levels of extractable PG activity and the rate of softening for a number of tomato cultivars (6, 13); (b) a lack of correlation between the activity of other cell wall enzymes (e.g. cellulase and A3-1,3-glucanase) and the rate of fruit softening (14, 29); (c) similarities between in vivo cell wall degradation products during ripening and in vitro cell wall products released by the action of purified PG on isolated fruit cell walls (15,30); and (d) the demonstration that slow ripening cultivars and nonripening tomato mutants are deficient in PG activity (6,22,28). Although these lines of evidence suggest a major role for PG in softening, other factors such as the levels of free and bound Ca2" in the cell wall and substrate accessibility may also be important (7).Recently, several groups have studied changes in gene expres- ' Supported by gifts from Chesebrough-

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“…It has often been suggested that PG is primarily responsible for pectin degradation and fruit softening (Hobson, 1965;Themmen, et al, 1982;Brady et al, 1983). However, PG-mediated pectin degradation alone cannot account for the significant changes in fruit texture that accompany ripening.…”

mentioning

confidence: 99%

A cDNA Clone Highly Expressed in Ripe Banana Fruit Shows Homology to Pectate Lyases

et al. 1997

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A cDNA clone (Banl7), encoding a protein homologous to pectate lyase, has been isolated from a cDNA library from climacteric banana fruit by means of differential screening. Northern analysis showed that Banl7 mRNA is first detected in early climacteric fruit, reaches a steady-state maximum at the climacteric peak, and declines thereafter in overripe fruit. Accumulation of the Banl7 transcript can be induced in green banana fruit by exogenous application of ethylene. This demonstrates that expression of this gene is under hormonal control, its induction being regulated by the rapid increase in ethylene production at the onset of ripening. The deduced amino acid sequence derived from the Banl7 cDNA shares significant identity with pectate lyases from pollen and plant pathogenic bacteria of the genus Erwinia. Similarity to bacterial pectate lyases that were proven to break down the pectic substances of the plant cell wall suggest that Banl7 might play a role in the loss of mesocarp firmness during fruit ripening.

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The Firmness of Tomato Fruit in Relation to Polygalacturonase Activity

Cited by 103 publications

References 0 publications

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Activity and expression of polygalacturonase vary at different fruit ripening stages of sweet pepper cultivars

Polygalacturonase Gene Expression in Rutgers, rin, nor, and Nr Tomato Fruits

A cDNA Clone Highly Expressed in Ripe Banana Fruit Shows Homology to Pectate Lyases

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