The fine structure of the midgut epithelium in Xerobiotus pseudohufelandi (Iharos, 1966) (Tardigrada, Eutardigrada, Macrobiotidae)

Rost‐Roszkowska, Magdalena; Poprawa, Izabela; Hyra, Marta; Marek-Swedziol, M.; Kaczmarek, Łukasz

doi:10.4081/jlimnol.2013.s1.e7

Cited by 8 publications

(14 citation statements)

References 39 publications

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“…Therefore, we can conclude that the stored material only plays the role of a food reserve for the period of starvation and the storage cells do not participate in the synthesis of vitellogenins in this species. It should be noted that the cells of the midgut epithelium of X. pseudohufelandi have previously been shown to accumulate a very small amount of food reserve (Rost‐Roszkowska et al ., ). However, huge multivesicular bodies, which play a role in the accumulation of water, were observed in the cytoplasm of the digestive cells in that study (Rost‐Roszkowska et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Twenty‐five adult specimens of each species ( H. dujardini , I. g. granulifer , M. polonicus , and X. pseudohufelandi ) were fixed in 2.5% glutaraldehyde in a 0.1 M phosphate buffer, pH 7.4, and postfixed in 2% osmium tetroxide in the same buffer. The fixation and subsequent dehydration were carried out in the same manner as was described previously (Rost‐Roszkowska, Poprawa & Kaczmarek, ; Rost‐Roszkowska et al ., ; Poprawa, Schlechte‐Wełnicz & Hyra, ) and lastly the material was embedded in an Epoxy Embedding Medium Kit (Sigma, St Louis, MO). Semi‐ (800 nm thick) and ultra‐thin (50 nm thick) sections were cut on a Leica Ultracut UCT25 ultramicrotome.…”

Section: Methodsmentioning

confidence: 99%

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Body cavity cells of Parachela during their active life

et al. 2016

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The body cavity cells (storage cells, storage bodies) of four species of Parachela (hermaphroditic Isohypsibius granulifer granulifer, parthenogenetic Hypsibius dujardini, gonochoristic Xerobiotus pseudohufelandi, gonochoristic Macrobiotus polonicus) were analysed during their active life using light, confocal (laser scanning), and scanning and transmission electron microscopy. The ultrastructure of the storage cells confirmed previous studies suggesting a high level of metabolic activity. Additionally, we revealed the participation of the storage cells of H. dujardini, I. g. granulifer, and M. polonicus in the synthesis of vitellogenins. This did not seem to apply for X. pseudohufelandi. All of the species that were examined in this study accumulated polysaccharides, proteins, and lipids in their body cavity cells, but the amount of these components differed in each species. Isohypsibius g. granulifer accumulated a huge amount of polysaccharides and smaller amounts of lipids and proteins, H. dujardini and M. polonicus primarily accumulated lipids and small amounts of polysaccharides and proteins, whereas X. pseudohufelandi primarily accumulated polysaccharides and lipids, and a small amount of proteins.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Body cavity cells of Parachela during their active life

et al. 2016

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“…Dehydration and embedding were performed as described earlier (Rost-Roszkowska et al 2013a, b; Poprawa et al 2015). Semi- and ultrathin sections were cut on a Leica ultracut UCT25 ultramicrotome.…”

Section: Methodsmentioning

confidence: 99%

The role of autophagy in the midgut epithelium of Parachela (Tardigrada)

2018

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The process of cell death has been detected in the midgut epithelium of four tardigrade species which belong to Parachela: Macrobiotus diversus, Macrobiotus polonicus, Hypsibius dujardini and Xerobiotus pseudohufelandi. They originated from different environments so they have been affected by different stressors: M. polonicus was extracted from a moss sample collected from a railway embankment; M. diversus was extracted from a moss sample collected from a petrol station; X. pseudohufelandi originated from sandy and dry soil samples collected from a pine forest; H. dujardini was obtained commercially but it lives in a freshwater or even in wet terrestrial environment. Autophagy is caused in the digestive cells of the midgut epithelium by different factors. However, a distinct crosstalk between autophagy and necrosis in tardigrades’ digestive system has been described at the ultrastructural level. Apoptosis has not been detected in the midgut epithelium of analyzed species. We also determined that necrosis is the major process that is responsible for the degeneration of the midgut epithelium of tardigrades, and “apoptosis–necrosis continuum” which is the relationship between these two processes, is disrupted.

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“…The single‐layered midgut epithelium is composed of the digestive cells (the principal cells); however, in the literature we only found information about the presence of regenerative cells that are described as ‘crescent‐like cells’ in Eutardigrada. These form the characteristic anterior epithelial ring of cells: ‘the crescent‐like cells’ (Marcus, ; Bertolani, ; Greven, ; Ząbczyk, ; Rost‐Roszkowska et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

“…To date, the structure and ultrastructure of the midgut epithelium has been studied in several tardigrade species (Marcus, ; Greven, ; Dewel, Roush & Dewel, ; Dewel et al ., ; Pirch & Greven, ; Ząbczyk, ; Avdonina et al ., ; Rost‐Roszkowska & Poprawa, ; Rost‐Roszkowska et al ., , ; Rost‐Roszkowska, Poprawa & Kaczmarek, ; Biserova & Mustafina, ); however, the relationship between changes in the midgut epithelium and changes in the gonad has only been described for the hermaphroditic Isohypsibius granulifer granulifer Thulin, 1928 (Rost‐Roszkowska et al ., ). The midgut epithelium of tardigrades is one of the tissues that, together with fat body, storage cells, trophocytes, and oocytes, are responsible for the synthesis of the yolk and chorion components, e.g.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural changes in the midgut epithelium ofHypsibius dujardini(Doyère, 1840) (Tardigrada, Eutardigrada, Hypsibiidae) in relation to oogenesis

et al. 2016

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In Hypsibius dujardini (Doy ere, 1840), the endodermal region of the digestive system, which is called the midgut, spreads along the entire length of the body. Its wall is formed by a simple epithelium that is composed of digestive cells. In this paper, we present the first report on the presence of two groups of midgut regenerative cells that form two 'epithelial rings'anterior and posterior. Additionally, we observed the proliferative abilities of the midgut regenerative cells, thus confirming the statement that they play the role of midgut stem cells. The precise ultrastructure of the digestive and regenerative cells was determined using transmission electron microscopy. Changes in the digestive and regenerative cells were correlated with the different stages of oogenesis. The process of oogenesis in H. dujardini took 4 days (at a temperature of 16°C). Reserve material gradually accumulated in the cytoplasm of the digestive cells and histochemical staining showed that it primarily contained proteins, polysaccharides and a small quantity of lipids. The reserve material accumulated during vitellogenesis, and it began to decrease during choriogenesis. During the simplex stage, when the entire buccalpharyngeal apparatus was expelled from the body, the stages of oogenesis were advanced, the midgut was much reduced, and the reserve material was exploited by the animal.

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The fine structure of the midgut epithelium in Xerobiotus pseudohufelandi (Iharos, 1966) (Tardigrada, Eutardigrada, Macrobiotidae)

Abstract: The

Cited by 8 publications

References 39 publications

Body cavity cells of Parachela during their active life

Body cavity cells of Parachela during their active life

The role of autophagy in the midgut epithelium of Parachela (Tardigrada)

Ultrastructural changes in the midgut epithelium ofHypsibius dujardini(Doyère, 1840) (Tardigrada, Eutardigrada, Hypsibiidae) in relation to oogenesis

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